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Sugars Profiles of Different Chestnut (Castanea sativa Mill.) and Almond (Prunus dulcis) Cultivars by HPLC-RI

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Abstract

Sugar profiles of different almond and chestnut cultivars were obtained by high-performance liquid chromatography (HPLC), by means of a refractive index (RI) detector. A solid-liquid extraction procedure was used in defatted and dried samples. The chromatographic separation was achieved using a Eurospher 100-5 NH2 column using an isocratic elution with acetonitrile/water (70:30, v/v) at a flow rate of 1.0 ml/min. All the compounds were separated in 16 min. The method was optimized and proved to be reproducible and accurate. Generally, more than 95% of sugars were identified for both matrixes. Sugars profiles were quite homogeneous for almond cultivars; sucrose was the main sugar (11.46 ± 0.14 in Marcona to 22.23 ± 0.59 in Ferragnes g/100 g of dried weight), followed by raffinose (0.71 ± 0.05 in Ferraduel to 2.11 ± 0.29 in Duro Italiano), glucose (0.42 ± 0.12 in Pegarinhos two seeded to 1.47 ± 0.19 in Ferragnes) and fructose (0.11 ± 0.02 in Pegarinhos two seeded to 0.59 ± 0.05 in Gloriette). Commercial cultivars proved to have higher sucrose contents, except in the case of Marcona. Nevertheless, chestnut cultivars revealed a high heterogeneity. Sucrose was the main sugar in Aveleira (22.05 ± 1.48), Judia (23.30 ± 0.83) and Longal (9.56 ± 0.91), while glucose was slightly prevalent in Boa Ventura (6.63 ± 0.49). The observed variance could serve for inter-cultivar discrimination.

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Abbreviations

FAO:

Food and Agriculture Organization

HPLC:

High-performance liquid chromatography

IS:

Internal standard

PDO:

Protected designation of origin

RI:

Refractive index

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Acknowledgements

Foundation for Science and Technology (Portugal) for financial support to J.C.M. Barreira (SFRH/BD/29060/2006) and INTERREG IIIA project PIREFI.

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Authors and Affiliations

  1. CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 1172, 5301-855, Bragança, Portugal

    João C. M. Barreira, José Alberto Pereira & Isabel C. F. R. Ferreira

  2. REQUIMTE/Serviço de Bromatologia, Faculdade de Farmácia da Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030, Porto, Portugal

    João C. M. Barreira & M. Beatriz P. P. Oliveira

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  1. João C. M. Barreira
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  2. José Alberto Pereira
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  3. M. Beatriz P. P. Oliveira
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  4. Isabel C. F. R. Ferreira
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Correspondence to Isabel C. F. R. Ferreira.

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Barreira, J.C.M., Pereira, J.A., Oliveira, M.B.P.P. et al. Sugars Profiles of Different Chestnut (Castanea sativa Mill.) and Almond (Prunus dulcis) Cultivars by HPLC-RI. Plant Foods Hum Nutr 65, 38–43 (2010). https://doi.org/10.1007/s11130-009-0147-7

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