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A panel of microRNAs as a new biomarkers for the detection of deep vein thrombosis

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Abstract

Deep vein thrombosis is one of the common complications of orthopedic surgery, and pulmonary embolism which is one of its lethal complications can lead to mortality. Numerous efforts have been made to identify reliable and predictive biomarkers to detect the early signs of deep vein thrombosis. These studies have, however, not delivered any more informative candidates than the D-dimer that have been available. Cell-free microRNAs are present in a range of body fluids and have recently been shown to be useful biomarkers in many diseases. Therefore, the purpose of present study was to identify potential microRNA biomarkers of deep vein thrombosis that are present in serum. Serum samples were taken from 18 deep vein thrombosis patients and 20 age- and sex-matched controls. TaqMan microRNA array was used for an initial screening. Real-time PCR assay was implemented to confirm the concentrations of candidate microRNAs. We found that the serum levels of miR-582, miR-195 and miR-532 of deep vein thrombosis patients were higher than those of controls. miR-582 yielded an AUC (the areas under the ROC curve) of 0.959, and the other two microRNAs yielded an AUC of 1.000 in discriminating deep vein thrombosis from controls. These data hint that serum miR-582, miR-195 and miR-532 might have potential to be a novel noninvasive biomarkers for detection of DVT. And this is the first study suggesting that serum microNRAs might be used as biomarkers for deep vein thrombosis.

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Acknowledgments

The authors would like to thank Chenyu Zhang (Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University) for technical assistance and English editing. This work was supported by grants from the National Natural Sciences of China (No. 30973046, No. 81271945), and China National Funds for Distinguished Young Scientists (No. 81125013).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Zhongshan Road 321, Nanjing, 210008, Jiangsu, People’s Republic of China

    Jizheng Qin, Dongquan Shi, Jin Dai, Zhihong Xu, Dongyang Chen & Qing Jiang

  2. Joint Research Center for Bone and Joint Disease, Model Animal Research Center (MARC), Nanjing University, Nanjing, Jiangsu, People’s Republic of China

    Jizheng Qin, Dongquan Shi, Jin Dai, Zhihong Xu, Dongyang Chen & Qing Jiang

  3. Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, People’s Republic of China

    Hongwei Liang & Xi Chen

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  1. Jizheng Qin
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  2. Hongwei Liang
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  3. Dongquan Shi
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  8. Qing Jiang
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Correspondence to Qing Jiang.

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Jizheng Qin and Hongwei Liang contributed equally to this manuscript.

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Qin, J., Liang, H., Shi, D. et al. A panel of microRNAs as a new biomarkers for the detection of deep vein thrombosis. J Thromb Thrombolysis 39, 215–221 (2015). https://doi.org/10.1007/s11239-014-1131-0

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  • DOI: https://doi.org/10.1007/s11239-014-1131-0

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