Skip to main content
Log in

Infection of cattle in Kenya with Brucella abortus biovar 3 and Brucella melitensis biovar 1 genotypes

  • Brief Communication
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Brucella melitensis biovar 1 was isolated from bovine milk samples from a herd in central Kenya, and Brucella abortus biovar 3 was isolated from aborted fetus materials and vaginal discharge fluids from cattle in central and eastern provinces of Kenya. All infections including those with B. melitensis were in cattle with reproductive problems kept in mixed herds indicating that cross infection occurs from small ruminants. Multiple-locus variable-number tandem repeat analysis genotyping revealed a close molecular homology of the B. melitensis isolates with an isolate from Israel and a close homology of the B. abortus isolates with an isolate from Uganda indicating that these genotypes have a wide geographic distribution. Infection of cattle with B. melitensis may complicate the control of brucellosis in this country.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Aggad, H. and Boukraa, L., 2006. Prevalence of bovine and human brucellosis in western Algeria: comparison of screening tests, East Mediterranean Health Journal, 12, 119–128

    CAS  Google Scholar 

  • Al Dahouk, S., Le Flèche, P.L., Nöckler, K., Jacques, I., Grayon, M., Scholz, H.C., Tomaso, H., Vergnaud, G. and Neubauer, H., 2007. Evaluation of Brucella MLVA typing for human brucellosis, Journal of Microbiology Methods, 69, 137–145

    Article  CAS  Google Scholar 

  • Alton, G.G., Jones, L.M., Angus, R.D. and Verger, J.M., 1988. Techniques for the brucellosis laboratory, Institut National de la Recherche Agronomique, Paris, France. pp 63–129

  • Alvarez, J., Sáez, J.L., García, N., Serrat, C., Pérez-Sancho, M., González, S., Ortega, M.J., Gou, J., Carbajo, L., Garrido, F., Goyache, J. and Domínguez, L., 2011. Management of an outbreak of brucellosis due to B. melitensis in dairy cattle in Spain, Research of Veterinary Science, 90, 208–211

  • Arimi, S.M., Koroti, E., Kang'ethe, E.K., Omore, A.O. and McDermott, J.J., 2005. Risk of infection with Brucella abortus and Escherichia coli O157:H7 associated with marketing of unpasteurized milk in Kenya, Acta Tropica, 96, 1–8

    Article  PubMed  CAS  Google Scholar 

  • Banai, M., 2010. Insights into the problem of B. melitensis and rationalizing a vaccination programme in Israel, Prilozi, 31, 167–180

    PubMed  CAS  Google Scholar 

  • Bankole, A. A., Saegerman, C., Berkvens, D., Fretin, D., Geerts, S., Ieven, G. and Walravens, K., 2010. Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia, Veterinary Record 166, 753–756

    Article  PubMed  CAS  Google Scholar 

  • Cutler, S.J., Whatmore, A.M. and Commander, N.J., 2005. Brucellosis--new aspects of an old disease, Journal of Applied Microbiology, 98, 1270–1281

    Article  PubMed  CAS  Google Scholar 

  • Fischer-Tenhagen, C., Hamblin, C., Quandt, S. and Frölich, K., 2000. Serosurvey for selected infectious disease agents in free-ranging black and white rhinoceros in Africa, Journal of Wildlife Diseases, 36, 316–323

    PubMed  CAS  Google Scholar 

  • Franco, M.P., Mulder, M., Gilman, R.H. and Smits, H.L., 2007. Human brucellosis, Lancet Infectious Disease, 12, 775–786

    Article  Google Scholar 

  • Godfroid, J., Cloeckaert, A., Liautard, J.P., Kohler, S., Fretin, D., Walravens, K., Garin-Bastuji, B. and Letesson, J.J., 2005. From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis, Veterinary Research. 36, 313–326

    Article  PubMed  Google Scholar 

  • Ica, T., Aydin, F., Erdenlig, S., Guler, L. and Büyükcangaz, E., 2008. Characterisation of Brucella abortus biovar 3 isolates from Turkey as biovar 3b, Veterinary Record, 163, 659–661

    Article  PubMed  CAS  Google Scholar 

  • Kadohira, M., McDermott, J.J., Shoukri, M.M. and Kyule, M.N., 1997. Variations in the prevalence of antibody to Brucella infection in cattle by farm, area and district in Kenya, Epidemiology Infection, 118, 35–41

    Article  CAS  Google Scholar 

  • Kang'ethe, E.K., Ekuttan, C.E., Kimani, V.N. and Kiragu, M.W., 2007. Investigations into the prevalence of bovine brucellosis and the risk factors that predispose humans to infection among urban dairy and non-dairy farming households in Dagoretti Division, Nairobi, Kenya, East African Medical Journal, 84, S96–100

    Google Scholar 

  • Kattar, M.M., Jaafar, R.F., Araj, G.F., Le Flèche, P., Matar, G.M., Abi Rached, R., Khalife, S. and Vergnaud, G., 2008. Evaluation of a multi locus variable-number tandem-repeat analysis scheme for typing human Brucella isolates in a region of brucellosis endemicity, Journal of Clinical Microbiology, 46, 3935–3940

    Article  PubMed  Google Scholar 

  • Le Flèche, P., Jacques, I., Grayon, M., Al Dahouk, S., Bouchon, P., Denoeud, F., Nöckler, K., Neubauer, H., Guilloteau, L.A. and Vergnaud, G., 2006. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay, BMC Microbiology, 6, 9

    Article  PubMed  Google Scholar 

  • Maichomo, M.W., McDermott, J.J., Arimi, S.M., Gathura, P.B., Mugambi, T.J. and Muriuki, S.M., 2000. Study of brucellosis in a pastoral community and evaluation of the usefulness of clinical signs and symptoms in differentiating it from other flu-like diseases, African Journal of Health Science, 7, 114–119

    CAS  Google Scholar 

  • Matope, G., Bhebhe, E., Muma, J.B., Skjerve, E. and Djønne, B., 2009. Characterization of some Brucella species from Zimbabwe by biochemical profiling and AMOS-PCR, BMC Research Notes, 2, 261

    Article  PubMed  Google Scholar 

  • McDermott, J.J. and Arimi, S.M., 2002. Brucellosis in sub-Saharan Africa: epidemiology, control and impact, Veterinary Microbiology, 90, 111–134

    Article  PubMed  Google Scholar 

  • Namanda, A.T., Kakai, R. and Otsyula, M., 2009. The role of unpasteurized "hawked" milk in the transmission of brucellosis in Eldoret municipality, Kenya, The Journal of Infection in Developing Countries, 3, 260–266

    Google Scholar 

  • Nöckler, K., Maves, R., Cepeda, D., Draeger, A., Mayer-Scholl, A., Chacaltana, J., Castañeda, M., Espinosa, B., Castillo, R., Hall, E., Al Dahouk, S., Gilman, R.H., Cabeza, F. and Smits, H.L., 2009. Molecular epidemiology of Brucella genotypes in patients at a major hospital in central Peru, Journal of Clinical Microbioogy, 47, 3147–3155

    Article  Google Scholar 

  • Ocholi, R.A., Kwaga, J.K., Ajogi, I. and Bale, J.O., 2004. Phenotypic characterization of Brucella strains isolated from livestock in Nigeria, Veterinary Microbiology, 103, 47–53

    Article  PubMed  CAS  Google Scholar 

  • Paling, R.W., Waghela, S., Macowan, K.J. and Heath, B.R., 1988. The occurrence of infectious diseases in mixed farming of domesticated wild herbivores and livestock in Kenya. II. Bacterial diseases, Journal of Wildlife Diseases, 24, 308–316

    PubMed  CAS  Google Scholar 

  • Samaha, H., Al-Rowaily, M., Khoudair, R.M. and Ashour, H.M., 2008. Multicenter study of brucellosis in Egypt, Emerging Infectious Disease, 14, 1916–1918

    Article  Google Scholar 

  • Smits, H.L., Espinosa, B., Castillo, R., Hall, E., Guillen, A., Zevaleta, M., Gilman, R.H., Melendez, P., Guerra, C., Draeger, A., Broglia, A. and Nöckler, K., 2009. Brucella MLVA genotyping of human Brucella isolates from Peru, Transaction of The Royal Society of Tropical Medicine and Hygiene, 103, 399–402.

    Article  CAS  Google Scholar 

  • Waghela, S. and Karstad, L., 1986. Antibodies to Brucella spp. among blue wildebeest and African buffalo in Kenya, Journal of Wildlife Diseases, 22, 189–192.

    PubMed  CAS  Google Scholar 

  • Whatmore, A.M., Shankster, S.J., Perrett, L.L., Murphy, T.J., Brew, S.D., Thirlwall, R.E., Cutler, S.J. and MacMillan, A.P., 2006. Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp., Journal of Clinical Microbiology, 44, 1982–1993

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgment

The authors would like to thank the Director of the Veterinary Services, Nairobi, Kenya for his kind support and interest in the project. Esther Muendo was a fellow of IEAE and NUFFIC and we kindly acknowledge their contribution to this project through grant no. CK/KEN/10001.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Henk L. Smits.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Muendo, E.N., Mbatha, P.M., Macharia, J. et al. Infection of cattle in Kenya with Brucella abortus biovar 3 and Brucella melitensis biovar 1 genotypes. Trop Anim Health Prod 44, 17–20 (2012). https://doi.org/10.1007/s11250-011-9899-9

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-011-9899-9

Keywords

Navigation