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Molecular pathotyping and phylogenesis of the first Newcastle disease virus strain isolated from backyard chickens in Qatar

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Abstract

The first Newcastle disease virus (NDV) field strain (NDV-QC08), previously isolated and identified in Qatar, was further subjected to molecular characterization and phylogenic analysis at passage levels two. Initial amplification and detection of the complementary DNA (cDNA) fragment of the NDV RNA amnioallantoic fluid (AAF) extract demonstrated the positive virus identity using real-time reverse transcriptase chain reaction (RRT-PCR). Partial sequence of the totally amplified 216 bp of the functional cleavage region encoding the F gene retrieved 112R-R-Q-K-R-F117 amino acid motif indicating the velogenic nature of the strain, and confirming the previously investigated in-vivo pathotyping. Phylogenic analysis and genotypic clustering studies classified the strain under NDV subgenotype VIId.

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Abbreviations

ND:

Newcastle disease

NDV:

Newcastle disease virus

RRT-PCR:

Real-time reverse transcription polymerase chain reaction

RT-PCR:

Reverse transcription polymerase chain reaction

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Acknowledgments

This study was conducted in Virology and Molecular Biology Units, Veterinary Laboratory, Department of Animal Resources. The conclusive molecular analysis was performed at the Department of Population Medicine, Faculty of Veterinary Medicine, University of Cornell, USA. The authors are grateful to the authorities of these institutions using those facilities.

Ethical declaration

This is to certify that the authors of this submitted manuscript have been performed the study in accordance with the ethical standards laid down in the 1964 Helsinki Declaration and its later amendments. As well, all authors approved their consent prior to conduction of the investigation.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Virology Unit, Veterinary Laboratory, Department of Animal Resources, P.O. Box 23211, Doha, Qatar

    Mohamed Haroun

  2. Molecular Biology Unit, Veterinary Laboratory, Department of Animal Resources, P.O. Box 23211, Doha, Qatar

    Khalid Abdelhakeem Mohran

  3. Veterinary Services, Department of Animal Resources, P.O. Box 23211, Doha, Qatar

    Mahmoud Mahmoud Hassan

  4. Molecular Biology Unit, Veterinary Laboratory, Department of Animal Resources, P.O. Box 23211, Doha, Qatar

    Nawal Mohamed Abdulla

Authors
  1. Mohamed Haroun
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  2. Khalid Abdelhakeem Mohran
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  3. Mahmoud Mahmoud Hassan
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  4. Nawal Mohamed Abdulla
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Corresponding author

Correspondence to Mohamed Haroun.

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Haroun, M., Mohran, K.A., Hassan, M.M. et al. Molecular pathotyping and phylogenesis of the first Newcastle disease virus strain isolated from backyard chickens in Qatar. Trop Anim Health Prod 47, 13–19 (2015). https://doi.org/10.1007/s11250-014-0677-3

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  • DOI: https://doi.org/10.1007/s11250-014-0677-3

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