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Variable Regions 1 and 2 (VR1 and VR2) in JSRV gag Are Not Responsible for the Endogenous JSRV Particle Release Defect

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Abstract

Jaagsiekte sheep retrovirus (JSRV) is a betaretrovirus causing ovine pulmonary adenocarcinoma, a transmissible lung tumor of sheep. A very closely related endogenous retrovirus (enJSRV) occurs as 15 to 20 copies in the genome of all sheep, and is not known to be linked to pathogenesis. We previously localized a particle release defect of the full-length endogenous-derived expression construct pCMV2enJS56A1 to the amino-terminal region of gag that incorporates the two variable regions VR1 and VR2, which harbor the main sequence differences between endogenous and exogenous JSRV in this part of gag. Here, we tested the hypothesis that either or both of these variable regions are responsible for the observed particle release defect in enJS56A1. We found that the PPPPPPPS motif of the exogenous VR1 is neither necessary nor sufficient for particle release. Furthermore, the precise substitution of VR1 and VR2 in the exogenous JSRV expression plasmid pCMV2JS 21, using their enJS56A1-derived counterparts, did not abrogate the ability of the resulting constructs to release particles. The particle release defect of enJS56A1 is therefore not determined exclusively by either VR1 or VR2. These results point to a small number of amino acids lying outside of VR1 and VR2 that may be responsible for the particle defect of enJS56A1 Gag.

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References

  • J.C. DeMartini J.V. Bishop T.E. Allen F.A. Jassim J.M. Sharp M. De las Heras D.R. Voelker J O. Carlson (2001) J Virol 75 4239–4246 Occurrence Handle1:CAS:528:DC%2BD3MXivFOkt7c%3D Occurrence Handle11287573 Occurrence Handle114169 Occurrence Handle10.1128/JVI.75.9.4239-4246.2001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • M. Palmarini J.M. Sharp M. De las Heras H. Fan (1999) J Virol 73 6964–6972 Occurrence Handle1:CAS:528:DyaK1MXksFKmsrk%3D Occurrence Handle10400795 Occurrence Handle112782

    CAS  PubMed  PubMed Central  Google Scholar 

  • D.F. York R. Vigne D.W. Verwoerd G. Querat (1992) J Virol 66 4930–4939 Occurrence Handle1:CAS:528:DyaK3sXksVaht7Y%3D Occurrence Handle1629959 Occurrence Handle241337

    CAS  PubMed  PubMed Central  Google Scholar 

  • M. Palmarini H. Fan (2001) J Natl Cancer Inst 93 1603–1614 Occurrence Handle1:CAS:528:DC%2BD3MXptVegt7w%3D Occurrence Handle11698564 Occurrence Handle10.1093/jnci/93.21.1603

    Article  CAS  PubMed  Google Scholar 

  • K. Perk I. Hod (1982) J Natl Cancer Inst 69 747–749 Occurrence Handle1:STN:280:DyaL3s%2FhtVyktg%3D%3D Occurrence Handle6288989

    CAS  PubMed  Google Scholar 

  • S.J. Hecht J.O. Carlson J.C. DeMartini (1994) Virology 202 480–484 Occurrence Handle1:CAS:528:DyaK2cXkslOqtr8%3D Occurrence Handle8009860 Occurrence Handle10.1006/viro.1994.1366

    Article  CAS  PubMed  Google Scholar 

  • S.J. Hecht K.E. Stedman J.O. Carlson J.C. DeMartini (1996) Proc Natl Acad Sci U S A 93 3297–3302 Occurrence Handle1:CAS:528:DyaK28XisVWisL0%3D Occurrence Handle8622932 Occurrence Handle39601 Occurrence Handle10.1073/pnas.93.8.3297

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • M. Palmarini C. Hallwirth D. York C. Murgia T. de Oliveira T. Spencer H. Fan (2000) J Virol 74 8065–8076 Occurrence Handle1:CAS:528:DC%2BD3cXlsl2nsrY%3D Occurrence Handle10933716 Occurrence Handle112339 Occurrence Handle10.1128/JVI.74.17.8065-8076.2000

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • J. Bai R.Y. Zhu K. Stedman C. Cousens J. Carlson J.M. Sharp J.C. DeMartini (1996) J Virol 70 3159–3168 Occurrence Handle1:CAS:528:DyaK28Xit1Ogsr4%3D Occurrence Handle8627796 Occurrence Handle190179

    CAS  PubMed  PubMed Central  Google Scholar 

  • M. Palmarini C. Cousens R.G. Dalziel J. Bai K. Stedman J.C. DeMartini J.M. Sharp (1996) J Virol 70 1618–1623 Occurrence Handle1:CAS:528:DyaK28XpvFanug%3D%3D Occurrence Handle8627682 Occurrence Handle189985

    CAS  PubMed  PubMed Central  Google Scholar 

  • A. Alberti C. Murgia S.L. Liu M. Mura C. Cousens M. Sharp A.D. Miller M. Palmarini (2002) J Virol 76 5387–5394 Occurrence Handle1:CAS:528:DC%2BD38XjslGnt7c%3D Occurrence Handle11991967 Occurrence Handle137058 Occurrence Handle10.1128/JVI.76.11.5387-5394.2002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • M. Palmarini N. Maeda C. Murgia C. De Fraja A. Hofacre H. Fan (2001) J Virol 75 11002–11009 Occurrence Handle1:CAS:528:DC%2BD3MXnvVeltL0%3D Occurrence Handle11602740 Occurrence Handle114680 Occurrence Handle10.1128/JVI.75.22.11002-11009.2001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • E.J. Blott G. Bossi R. Clark M. Zvelebil G.M. Griffiths (2001) J Cell Sci 114 2405–2416 Occurrence Handle1:CAS:528:DC%2BD3MXlsFamtL0%3D Occurrence Handle11559749

    CAS  PubMed  Google Scholar 

  • S. Hong G. Choi S. Park A.S. Chung E. Hunter S.S. Rhee (2001) J Virol 75 2526–2534 Occurrence Handle1:CAS:528:DC%2BD3MXhsFWitLs%3D Occurrence Handle11222675 Occurrence Handle115874 Occurrence Handle10.1128/JVI.75.6.2526-2534.2001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • L.E. Stahl R.L. Wright J.D. Castle A.M. Castle (1996) J Cell Sci 109 1637–1645 Occurrence Handle1:CAS:528:DyaK28XktVals7k%3D Occurrence Handle8799850

    CAS  PubMed  Google Scholar 

  • M.P. Williamson (1994) Biochem J 297 249–260 Occurrence Handle1:CAS:528:DyaK2cXpvFGguw%3D%3D Occurrence Handle8297327 Occurrence Handle1137821 Occurrence Handle10.1042/bj2970249

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • B. Yuan X. Li S.P. Goff (1999) EMBO J 18 4700–4710 Occurrence Handle1:CAS:528:DyaK1MXmt1yisbw%3D Occurrence Handle10469649 Occurrence Handle1171543 Occurrence Handle10.1093/emboj/18.17.4700

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • E.O. Freed (2002) J Virol 76 4679–4687 Occurrence Handle1:CAS:528:DC%2BD38Xjtlertbs%3D Occurrence Handle11967285 Occurrence Handle136167 Occurrence Handle10.1128/JVI.76.10.4679-4687.2002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • E. Gottwein J. Bodem B. Muller A. Schmechel H. Zentgraf H.G. Krausslich (2003) J Virol 77 9474–9485 Occurrence Handle1:CAS:528:DC%2BD2cXhsF2qsQ%3D%3D Occurrence Handle12915562 Occurrence Handle187385 Occurrence Handle10.1128/JVI.77.17.9474-9485.2003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • O. Pornillos J.E. Garrus W.I. Sundquist (2002) Trends Cell Biol 12 569–579 Occurrence Handle1:CAS:528:DC%2BD38XpsV2msbg%3D Occurrence Handle12495845 Occurrence Handle10.1016/S0962-8924(02)02402-9

    Article  CAS  PubMed  Google Scholar 

  • J. Sambrook E.F. Fritsch T. Maniatis (Eds) (1989) Molecular Cloning \ndash A Laboratory Manual, 2nd edn Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY

    Google Scholar 

  • M. Palmarini J.M. Sharp C. Lee H. Fan (1999) J Virol 73 10070–10078 Occurrence Handle1:CAS:528:DyaK1MXnsV2mtLc%3D Occurrence Handle10559321 Occurrence Handle113058

    CAS  PubMed  PubMed Central  Google Scholar 

  • M. Palmarini M.J. Holland C. Cousens R.G. Dalziel J.M. Sharp (1996) J Gen Virol 77 2991–2998 Occurrence Handle1:CAS:528:DyaK2sXhvVKgsg%3D%3D Occurrence Handle9000089 Occurrence Handle10.1099/0022-1317-77-12-2991

    Article  CAS  PubMed  Google Scholar 

  • M. Palmarini M. Mura T.E. Spencer (2004) J Gen Virol 85 1–13 Occurrence Handle1:CAS:528:DC%2BD2cXhtFGrsbs%3D Occurrence Handle14718613 Occurrence Handle10.1099/vir.0.19547-0

    Article  CAS  PubMed  Google Scholar 

  • U.K. von Schwedler M. Stuchell B. Muller D.M. Ward H.Y. Chung E. Morita H.E. Wang T. Davis G.P. He D.M. Cimbora A. Scott H.G. Krausslich J. Kaplan S.G. Morham W.I. Sundquist (2003) Cell 114 701–713 Occurrence Handle1:CAS:528:DC%2BD3sXnslKisb8%3D Occurrence Handle14505570 Occurrence Handle10.1016/S0092-8674(03)00714-1

    Article  CAS  PubMed  Google Scholar 

  • F. Bouamr J.A. Melillo M.Q. Wang K. Nagashima S.M. de Los A. Rein S.P. Goff (2003) J Virol 77 11882–11895 Occurrence Handle1:CAS:528:DC%2BD3sXovVansL0%3D Occurrence Handle14581525 Occurrence Handle253756 Occurrence Handle10.1128/JVI.77.22.11882-11895.2003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • R. Swanstrom J.W. Wills J.M. Coffin S.H. Hughes H.E. Varmus (Eds) (1997) Retroviruses Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY 263–334

    Google Scholar 

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Correspondence to Claus Hallwirth.

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Hallwirth, C., Maeda, N., York, D. et al. Variable Regions 1 and 2 (VR1 and VR2) in JSRV gag Are Not Responsible for the Endogenous JSRV Particle Release Defect. Virus Genes 30, 59–68 (2005). https://doi.org/10.1007/s11262-004-4582-0

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