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A Short Insert in the Leader Sequence of RNA 3L, A Long Variant of Alfalfa mosaic virus RNA3, Introduces Two Unidentified Reading Frames

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Abstract

N20-RNA 3L, a large form of RNA 3 associated with Alfalfa mosaic virus (AMV) strain N20 comprises 2281nt and has approximately 97% overall sequence similarity to the longest previously described RNA 3 of AMV strain YSMV (YSMV-RNA 3; 2188nt). Compared with YSMV-RNA 3, N20-RNA 3L contains an additional 97nt in the 5' leader upstream of the open reading frames for movement protein (MP) and coat protein (CP). Two overlapping unidentified reading frames (URF1 and URF2) result from this modification, each of which code for putative translation products of 21 amino acids. The URF1 putative peptide has a hydrophilic N-terminus and a hydrophobic C-terminus, indicating a possible association with both host cell membrane and cytosol whereas the putative URF2 product is predominantly hydrophobic. A further structural modification found in N20-RNA 3L is a new tandem repeat of 243nts which overlaps with the MP open reading frame.

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References

  1. Bol J. F., Mol Plant Path 4, 1–8, 2003.

    Google Scholar 

  2. Hajimorad M. R. and Francki R. I. B., Ann appl Biol 113, 45–54, 1988.

    Google Scholar 

  3. Hajimorad M. R., Kurath G., Randles J. W., and Francki, R. I. B., J Gen Virol 72, 2885–2893, 1991.

    PubMed  Google Scholar 

  4. Dore J.-M., Pinck, M., and Pinck L., J Gen Virol 70, 777–782, 1989.

    PubMed  Google Scholar 

  5. Peden K. W. C. and Symons R. H., Virology 53, 487–492, 1973.

    PubMed  Google Scholar 

  6. Symons R. H., Aust J Biol Sci 31, 25–37, 1978.

    Google Scholar 

  7. Jayasena K. W., Ingham B. J., Hajimorad M. R., and Randles J. W., Aust J Agric Res 48, 503–510, 1997.

    Google Scholar 

  8. Sambrook J., Fritsch E. F., and Maniatis T. A., Molecular Cloning: A Laboratory Manual 2nd edn. Cold Spring Harbor Laboratory Press, N. Y, 1989.

    Google Scholar 

  9. Neeleman L., van der Kuyl A. C., and Bol J. F., Virology 181, 687–693, 1991.

    PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Agriculture Western Australia, 444 Albany Highway, Western Australia, Australia

    Kithsiri Jayasena

  2. Plant and Pest Science, School of Agriculture and Wine, The University of Adelaide, Waite Campus, Glen Osmond, Australia

    John Randles

Authors
  1. Kithsiri Jayasena
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  2. John Randles
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Jayasena, K., Randles, J. A Short Insert in the Leader Sequence of RNA 3L, A Long Variant of Alfalfa mosaic virus RNA3, Introduces Two Unidentified Reading Frames. Virus Genes 29, 311–316 (2004). https://doi.org/10.1007/s11262-004-7433-0

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  • DOI: https://doi.org/10.1007/s11262-004-7433-0

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