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Identification and phylogenetic analysis of orf virus from goats in Taiwan

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Abstract

An outbreak of contagious ecthyma in goats in central Taiwan was investigated. The disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (PCR) and sequence of major envelope protein (B2L) gene. The entire protein-coding region of B2L gene were cloned and sequenced. Phylogenetic analysis of B2L amino acid sequences showed that the orf virus identified in this outbreak was closer to the Indian ORFV-Mukteswar 59/05 isolate. This is the first report on the molecular characterization of orf virus in Taiwan.

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Acknowledgement

We thank Dr. Shih-Chieh Chang (Department of Veterinary Medicine, National Chung-Hsing University) for helpful discussion. This work was supported in part by a grant from The Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ), Council of Agriculture, Taiwan.

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Correspondence to Min-Liang Wong.

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Chan, KW., Lin, JW., Lee, SH. et al. Identification and phylogenetic analysis of orf virus from goats in Taiwan . Virus Genes 35, 705–712 (2007). https://doi.org/10.1007/s11262-007-0144-6

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  • DOI: https://doi.org/10.1007/s11262-007-0144-6

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