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Molecular mutations associated with the in vitro passage of virulent porcine reproductive and respiratory syndrome virus

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Abstract

Mutants of a highly pathogenic, porcine reproductive, and respiratory syndrome virus (PRRSV), JXA1 strain, were prepared by continuous in vitro passage. Genomic sequence comparisons were made between mutants obtained at different passages and the parental strain JXA1. The mutant strain obtained at passage 80 contained a 12 nucleotide insertion and 108 nucleotide mutations that resulted in 45 amino acid changes. Most of these changes (89%) occurred between passage 10 and 45 and were genetically stable for the next 35–70 passages. A comparison of the mutants, their parental strain, and several American PRRSV strains, identified 13 characteristic amino acid changes. These sites, as well as the distinct 12 nucleotide insertion, represent possible genetic markers for the evaluation of live vaccine applications, particularly for additional studies of the safety and potency of live PRRSV vaccines.

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Acknowledgments

This work was supported by grants from the National Science and Technology Pillar Program in the 11th 5-year plan period of China (2007BAD86B01, 2007BAD86B03, 2007BAD86B04 and 2007BAD86B05).

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Authors and Affiliations

  1. College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan Western Road, Haidian District, Beijing, People’s Republic of China

    Wei Han, Ke-Gong Tian & Zhong-Qiu Zhang

  2. China Animal Disease Control Center, No. 20 Maizidian Rd., Chaoyang District, Beijing, People’s Republic of China

    Jia-Jun Wu, Xiao-Yu Deng, Zhen Cao, Xiu-ling Yu, Chuan-Bin Wang, Tie-Zhu Zhao, Nan-Hua Chen, Hong-Hui Hu, Wang Bin, Li-Li Hou, Li-Lin Wang, Ke-Gong Tian & Zhong-Qiu Zhang

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Correspondence to Ke-Gong Tian or Zhong-Qiu Zhang.

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Han, W., Wu, JJ., Deng, XY. et al. Molecular mutations associated with the in vitro passage of virulent porcine reproductive and respiratory syndrome virus. Virus Genes 38, 276–284 (2009). https://doi.org/10.1007/s11262-008-0322-1

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