Abstract
In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates contain endornavirus dsRNAs. Three endornavirus-specific RT–PCR amplicons were generated by RT–PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. The amplified segments showed a very high degree of sequence similarity suggesting that the virus has gone through a population bottleneck during its emergence.
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Acknowledgements
Z. Kozlakidis would like to thank The Wellcome Foundation for a VIP fellowship. A. Jamal thanks the Higher Education Commission of Pakistan for a PhD fellowship. All studies carried out on Phytophthora taxon douglasfir was under licence number PHL 189B/5412(06/2006) amended (10/2006) issued by DEFRA. We should like to thank Clive Brasier at the Forest Research Agency, Alice Holt Lodge, Farnham, Surrey, UK, and Beatrice Henricot at RHS Garden Wisley, Woking, Surrey, UK for supplying nitrogen-powdered mycelia of the licensed Phytophthora ramorum isolates examined.
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Zisis Kozlakidis and Neil A. Brown contributed equally to this study.
The nucleotide sequence data reported in this article has been assigned the accession numbers AM 941193, AM941200, AM941404; AM941194, AM941202, AM941402; AM941195, AM941201, AM941403; AM941196, AM941197, AM941410 for isolates P440, P458, P475, and 3543/04, respectively.
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Kozlakidis, Z., Brown, N.A., Jamal, A. et al. Incidence of endornaviruses in Phytophthora taxon douglasfir and Phytophthora ramorum . Virus Genes 40, 130–134 (2010). https://doi.org/10.1007/s11262-009-0421-7
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DOI: https://doi.org/10.1007/s11262-009-0421-7