Abstract
The complete sequence of a novel mycovirus infecting Ustilaginoidea virens, the causal agent of false smut of rice, is reported here and designated as Ustilaginoidea virens unassigned RNA virus HNND-1 (UvURV-HNND-1). This virus has an undivided dsRNA genome of 2903 nt in length and contains two non-overlapping open reading frames (ORF1 and 2), with the small ORF1 encoding a protein of unknown function that showed sequence similarity to the comparable protein in virus Alternaria longipes dsRNA virus 1(AlRV1) and a larger ORF2 encoded the protein showing identities to the RNA-dependent RNA polymerases of AlRV1 and some other unassigned dsRNA viruses. Phylogenetic analysis showed that UvURV-HNND-1 is more closely related to unclassified viruses such as AlRV1 and distinct from distantly related members of the family Partitiviridae. Here, we propose in accordance with previous reports that UvURV-HNND-1 might belong to a new mycovirus genus together with AlRV1 and other similar viruses.
Similar content being viewed by others
References
S.A. Ghabrial, N. Suzuki, Viruses of plant pathogenic fungi. Annu. Rev. Phytopathol. 47, 353–384 (2009)
M.N. Pearson, R.E. Beever, B. Boine, K. Arthur, Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol. Plant Pathol. 10, 115–128 (2009)
J. Xie, D.H. Jiang, New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu. Rev. Phytopathol. 52, 45–68 (2014)
D.L. Nuss, Hypovirulence: mycoviruses at the fungal-plant interface. Nat. Rev. Microbiol. 3, 632–642 (2005)
A.L. Dawe, D.L. Nuss, Hypoviruses and chestnut blight: exploiting viruses to understand and modulate fungal pathogenesis. Annu. Rev. Genet. 35, 1–29 (2001)
S. Chiba, L. Salaipeth, Y.H. Lin, A. Sasaki, S. Kanematsu, N. Suzuki, A novel bipartite double-stranded RNA mycovirus from the white root rot fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J. Virol. 83, 12801–12812 (2009)
M.C. Cooke, Some extra–European fungi. Grevillea 7, 13–15 (1878)
D. Ladhalakshmi, G. Laha, R. Singh, A. Karthikeyan, S. Mangrauthia, R. Sundaram, P. Thukkaiyannan, B. Viraktamath, Isolation and characterization of Ustilaginoidea virens and survey of false smut disease of rice in India. Phytoparasitica 40, 171–176 (2012)
R.F. Luduena, M.C. Roach, V. Prasad, M. Banerjee, Y. Koiso, Y. Li, S. Iwasaki, Interaction of ustiloxin A with bovine brain tubulin. Biochem. Pharmacol. 47, 1593–1599 (1994)
Y. Koiso, Y. Li, S. Iwasaki, K. Hanaoka, T. Kobayashi, R. Sonoda, Y. Fujita, H. Yaegashi, Z. Sato, Ustiloxins, antimitotic cyclic peptides from false smut balls onrice panicles caused by Ustilaginoidea virens. J. Antibiot. 47, 765–773 (1994)
T. Zhang, Y. Jiang, J. Huang, W. Dong, Complete genome sequence of a putative novel victorivirus from Ustilaginoidea virens. Arch. Virol. 6, 1403–1406 (2013)
T. Zhang, Y. Jiang, J. Huang, W. Dong, Genomic organization of a novel partitivirus from the phytopathogenic fungus Ustilaginoidea virens. Arch. Virol. 158, 2415–2419 (2013)
Y. Jiang, C. Luo, D. Jiang, G. Li, J. Huang, The complete genomic sequence of a second novel partitivirus infecting Ustilaginoidea virens. Arch. Virol. 159, 1865–1868 (2014)
J. Zhong, J.Z. Zhu, X.H. Lei, D. Chen, H.J. Zhu, B.D. Gao, Complete genome sequence and organization of a novel virus from the rice false smut fungus Ustilaginoidea virens. Virus Genes 48, 329–333 (2014)
J. Zhong, Q. Zhou, X.H. Lei, D. Chen, H.H. Shang, H.J. Zhu, The nucleotide sequence and genome organization of two victoriviruses from the rice false smut fungus Ustilaginoidea virens. Virus Genes 48, 570–573 (2014)
J. Zhong, X.H. Lei, J.Z. Zhu, G. Song, Y.D. Zhang, Y. Chen, B.D. Gao, Detection and sequence analysis of two novel co-infecting double-strand RNA mycoviruses in Ustilaginoidea virens. Arch. Virol. 159, 3063–3070 (2014)
T. Zhang, Y. Jiang, W. Dong, A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology 462, 227–235 (2014)
Y. Jiang, T. Zhang, C. Luo, D. Jiang, G. Li, Q. Li, J. Huang, Prevalence and diversity of mycoviruses infecting the plant pathogen Ustilaginoidea virens. Virus Res. 195, 47–56 (2015)
T.J. Morris, J.A. Dodds, Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathol 69, 854–858 (1979)
M.A. Larkin, G. Blackshields, N.P. Brown, R. Chenna, P.A. McGettigan, H. McWilliam, D.G. Higgins, Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947–2948 (2007)
K. Tamura, J. Dudley, M. Nei, S. Kumar, MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599 (2007)
Y. Lin, H. Zhang, C. Zhao, S. Liu, L. Guo, The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch. Virol. (2014). doi:10.1007/s00705-014-2218-9
I. Kotta-Loizou, J. Sipkova, R.H. Coutts, Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana. Arch. Virol. (2015). doi:10.1007/s00705-014-2332-8
Author information
Authors and Affiliations
Corresponding authors
Additional information
Edited By Thomas Hohn.
Hong Jian Zhu and Dan Chen contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zhu, H.J., Chen, D., Zhong, J. et al. A novel mycovirus identified from the rice false smut fungus Ustilaginoidea virens . Virus Genes 51, 159–162 (2015). https://doi.org/10.1007/s11262-015-1212-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-015-1212-y