Skip to main content
Log in

Intranasally administered Lactobacillus gasseri TMC0356 protects mice from H1N1 influenza virus infection by stimulating respiratory immune responses

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

We conducted a study to evaluate the possibility that intranasal administration of a new probiotic strain Lactobacillus gasseri TMC0356 (TMC0356) may protect host animals from influenza virus (IFV) infection, which was indicated by enhanced respiratory immune responses in a mouse model. After 3 days of exposure to TMC0356, BALB/c mice were intranasally infected with IFVA/PR/8/34 (H1N1). Lung cells were isolated from the tested mice and evaluated for cytotoxicity against YAC-1 cells. After intranasal treatment with TMC0356, mice showed a lower morbidity and higher survival rate compared to control mice (P < 0.05). The cytotoxicity of lung cells isolated from mice after intranasal treatment against YAC-1 cells was statistically higher than that of lung cells isolated from control mice (P < 0.05). Intranasal administration of TMC0356 significantly increased mRNA expression of interleukin (IL)-1β, tumor necrosis factor, IL-10, and monocyte chemotactic protein-1 (P < 0.01). These results suggest that intranasal administration of TMC0356 may protect the host animal from IFV infection. They also indicate that TMC0356 can enhance respiratory cell-mediated immune responses of host animals characteristically with up-regulated activation of lung natural killer cells. Further studies will evaluate the possible role of the immune stimulatory effects of TMC0356 within the protective effects of this bacterium against IFV, as observed in the present study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Allavena P, Bianchi G, Zho D, Damme JV, Jilek P, Sozzani S, Montovan A (2005) Induction of natural killer cell migration by monocyte chemotactic protein-1, 2α. Eur J Immunol 24:3233–3236

    Article  Google Scholar 

  • Couch RB (2008) Seasonal inactivated influenza virus vaccines. Vaccine 12(Suppl 4):D5–D9

    Article  Google Scholar 

  • FAO/WHO (2001) The joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food held in Córdoba, Argentina from 1–4 Oct 2001

  • Goldin BR, Gorbach SL (2008) Clinical indications for probiotics: an overview. Clin Infect Dis 1:S96–S100

    Article  Google Scholar 

  • Gostin LO (2004) Pandemic influenza: public health preparedness for next global health emergency. J Law Med Ethics 32:565–573

    Article  Google Scholar 

  • He F, Elina T, Heikki A, Seppo S (2000) Modulation of humoral immune response through probiotic intake. FEMS Immunol Med Microbiol 29:47–52

    Article  Google Scholar 

  • Hori T, Kiyoshima J, Shida K, Yasui H (2001) Effect of intranasal administration of Lactobacillus casei Shirota on influenza virus infection of upper respiratory tract in mice. Clin Diagn Lab Immunol 8:593–597

    CAS  Google Scholar 

  • Hori T, Kiyoshima J, Shida K, Yasui H (2002) Augmentation of cellular immunity and reduction of influenza virus titer in aged mice fed Lactobacillus casei strain Shirota. Clin Diagn Lab Immunol 9:105–108

    Google Scholar 

  • Hosoda M, Hashimoto H, He F, Yamazaki K, Hosono A (1998) Inhibitory effects of fecal Lactobacilli and Bifidobacteria on the mutagenicities of Trp-P-2 and IQ. Milchwissenschaft 53:309–313

    CAS  Google Scholar 

  • Hunter CA, Chizzonite R, Remington JS (1995) IL-1 beta is required for IL-12 to induce production of IFN-gamma by NK cells. A role for IL-1 beta in the T cell-independent mechanism of resistance against intracellular pathogens. J Immunol 155:4347–4354

    CAS  Google Scholar 

  • Kato I, Tanaka K, Yokokura T (1999) Lactic acid bacterium potently induces the production of interleukin-12 and interferon-gamma by mouse splenocytes. Int J Immunopharmacol 21:121–131

    Article  CAS  Google Scholar 

  • Kawase M, He F, Kubota A, Hata J, Kobayakawa S, Hiramatsu M (2006) Inhibitory effect of Lactobacillus gasseri TMC0356 and Lactobacillus GG on enhanced vascular permeability of nasal mucosa in experimental allergic rhinitis of rats. Biosci Biotechnol Biochem 70:3025–3030

    Article  CAS  Google Scholar 

  • Kawase M, He F, Kubota A, Harata G, Hiramatsu M (2007) Orally administrated Lactobacillus gasseri TMC0356 and Lactobacillus GG alleviated nasal blockage of guinea pig with allergic rhinitis. Microbiol Immunol 51:1109–1114

    CAS  Google Scholar 

  • Kawase M, He F, Kubota A, Hiramatsu M, Saito H, Ishii T, Yasueda H, Akiyama K (2009a) Effect of fermented milk prepared with 2 probiotic strains on Japanese cedar pollinosis in a double-blind placebo-controlled clinical study. Int J Food Microbiol 128:429–434

    Article  CAS  Google Scholar 

  • Kawase M, He F, Kubota A, Harata G, Hiramatsu M (2009b) Clinical effects of cell preparation of Lactobacillus GG and L. gasseri TMC0356 on perennial allergic rhinitis: a double-blind placebo-controlled trial. Int J Probiotics Prebiotics 4:241–248

    Google Scholar 

  • Loetscher P, Seitz M, Clark-Lweis I, Baggiolini M, Moser B (1996) Activation of NK cells by CC chemokines. Chemotaxis, Ca2+ mobilization, and enzyme release. J Immunol 156:322–327

    CAS  Google Scholar 

  • Lui KJ, Kendal AP (1987) Impact of influenza epidemics on mortality in the United States from October 1972 to May 1985. AJPH 77:712–716

    Article  CAS  Google Scholar 

  • Matsuzaki T, Chin J (2000) Modulating immune responses with probiotic bacteria. Immunol Cell Biol 78:67–73

    Article  CAS  Google Scholar 

  • Morita H, He F, Fuse T, Ouwehand AC, Hashimoto H, Hosoda M, Mizumachi K, Kurisaki J (2002) Cytokine production by the murine macrophage cell line J774.1 after exposure to lactobacilli. Biosci Biotechnol Biochem 66:1963–1966

    Article  CAS  Google Scholar 

  • Morita H, He F, Kawase M, Kubota A, Hiramatsu M, Kurisaki J, Salminen S (2006) Preliminary human study for possible alteration of serum immunoglobulin E production in perennial allergic rhinitis with fermented milk prepared with Lactobacillus gasseri TMC0356. Microbiol Immunol 50:701–706

    CAS  Google Scholar 

  • Peakman M, Vergani D (1997) Basic and clinical immunology. Churchill Liveingstone, Hong Kong, pp 131–146

    Google Scholar 

  • Sabel MS, Arora A, Su G, Mathiowitz E, Reineke JJ, Chang AE (2007) Synergistic effect of intratumoral IL-12 and TNF-alpha microspheres: systemic anti-tumor immunity is mediated by both CD8 + CTL and NK cells. Surgery 142:749–760

    Article  Google Scholar 

  • Saxelin M, Salminen S, Isolauri E (1998) Clinical efficacy of a human Lactobacillus strain as a probiotic in functional foods. In: Sadler MJ, Saltmarsh M (eds) The consumer, the products and the evidence. The Royal Society of Chemistry, London, Great Britain, pp 521–548

    Google Scholar 

  • Shida K, Suzuki T, Kiyoshima SJ, Shimada S, Nanno M (2006) Essential roles of monocytes in stimulating human peripheral blood mononuclear cells with Lactobacillus casei to produce cytokines and augment natural killer cell activity. Clin Vaccine Immunol 13:997–1003

    Article  CAS  Google Scholar 

  • Takeda K, Suzuki T, Shimada SI, Shida K, Nanno M, Okumura K (2006) Interleukin-12 is involved in the enhancement of human natural killer cell activity by Lactobacillus casei Shirota. Clin Exp Immunol 146:109–115

    Article  CAS  Google Scholar 

  • Wang Q, Pan J, Kawase M, Hiramatsu M, He F (2009) Orally administrated Lactobacillus gasseri TMC0356 inhibits growth of tumors transplanted into Mice. Biosci Microflora 28:39–43

    Google Scholar 

  • Yasui H, Kiyoshima J, Hori T, Shida K (1999) Protection against influenza virus infection of mice fed Bifidobacterium breve YIT4064. Clin Diagn Lab Immunol 6:186–192

    CAS  Google Scholar 

  • Yasui H, Kiyoshima J, Hori T (2004) Reduction of influenza virus titer and protection against influenza virus infection in infant mice fed Lactobacillus casei Shirota. Clin Diagn Lab Immunol 11:675–679

    Google Scholar 

Download references

Acknowledgments

This work was supported by a Grant-in-Aid for Research and Development from the Japanese Ministry of Agriculture and Forestry.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Fang He.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harata, G., He, F., Hiruta, N. et al. Intranasally administered Lactobacillus gasseri TMC0356 protects mice from H1N1 influenza virus infection by stimulating respiratory immune responses. World J Microbiol Biotechnol 27, 411–416 (2011). https://doi.org/10.1007/s11274-010-0472-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-010-0472-x

Keywords

Navigation