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Immunorelevant proteins for the diagnosis of bovine staphylococcal mastitis

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Abstract

Staphylococcus aureus is a leading cause of bovine mastitis, a condition in which the udder of the cow is inflamed, reducing the quality and quantity of milk produced. Staphylococcal mastitis is a common infection that can develop into a chronic form. The segregation of infected animals is an important preventive practice but relies on an effective diagnostic method. For this purpose, we constructed a genomic library of S. aureus, and a screening step was conducted with antiserum produced using the total protein extract of the pathogen. The nucleotide sequences of the immunoselected clones were aligned with the genome of bovine S. aureus RF122, which enabled the identification of 65 different loci, including proteins related to metabolism, adhesion and cell wall production, toxins, regulatory proteins, and hypothetical proteins. The subcellular location of the immunoreactive polypeptides was also determined. Fifty-two percent were cytoplasmic, 34 % were located in areas exposed to the host’s immune system, and for 14 %, the location could not be determined. In silico analysis of the presence of these proteins in mastitis pathogens showed that Fib, ClfA, and the hypothetical protein SAB0166 were the only proteins specific for S. aureus. Therefore, these proteins are promising candidates for the serodiagnosis of staphylococcal mastitis.

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Acknowledgments

We sincerely thank Dr. Maria Aparecida Paiva Brito from Embrapa Dairy Cattle for providing the bacterial strains used in this study. This work financially supported by Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). M.H. Fabres-Klein and R.C. Klein gratefully acknowledge the receipt of scholarships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Laboratory of Molecular Biotechnology, Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Brazil

    M. H. Fabres-Klein, R. C. Klein & A. O. B. Ribon

  2. Laboratory of Molecular Virology, Department of General Biology, Federal University of Viçosa, Viçosa, Brazil

    S. O. De Paula

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  1. M. H. Fabres-Klein
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  2. R. C. Klein
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  3. S. O. De Paula
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  4. A. O. B. Ribon
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Correspondence to A. O. B. Ribon.

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Fabres-Klein, M.H., Klein, R.C., De Paula, S.O. et al. Immunorelevant proteins for the diagnosis of bovine staphylococcal mastitis. World J Microbiol Biotechnol 29, 1155–1160 (2013). https://doi.org/10.1007/s11274-013-1274-8

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  • DOI: https://doi.org/10.1007/s11274-013-1274-8

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