Abstract
Introduction
The development of common forms of diabetes comes from the interaction between environmental and genetic factors, and the consequences of poor glycemic control in these patients could result in several complications. Metabolomic studies for type 2 diabetes mellitus in serum/plasma have reported changes in numerous metabolites, which might be considered possible targets for future mechanistic research. However, the specific role of a particular metabolite as cause or consequence of diabetes derangements is difficult to establish.
Objectives
As type 2 diabetes is a disease that changes the metabolic profile in several levels, this work aimed to compare the metabolomic profiles of type 2 diabetes mellitus and non-diabetic participants. In addition, we exploited our family-based study design to bring a better understanding of the causal relationship of identified metabolites and diabetes.
Methods
In the current study, population based metabolomics was applied in 939 subjects from the Baependi Heart Study. Participants were separated into two groups: diabetic (77 individuals) and non-diabetic (862 individuals), and the metabolic profile was performed by GC/MS technique.
Results
We have identified differentially concentrated metabolites in serum of diabetic and non-diabetic individuals. We identified 72 metabolites up-regulated in type 2 diabetes mellitus compared to non-diabetics. It was possible to recapitulate the main pathways that the literature shows as changed in diabetes. Also, based on metabolomic profile, we separated pre-diabetic individuals (with glucose concentration between 100–125 mg/dL) from non-diabetics and diabetics. Finally, using heritability analysis, we were able to suggest metabolites in which altered levels may precede diabetic development.
Conclusion
Our data can be used to derive a better understanding of the causal relationship of the observed associations and help to prioritize diabetes-associated metabolites for further work.
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Acknowledgments
We acknowledge the Agilent Technologies Brasil Ltda, Life Sciences & Chemical Analysis for the use of the GC/MS system (7890B gas chromatograph coupled to a mass selective detector model 5977A-Agilent) for support data collection.
Funding information
This work was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP 2012/05447-0, 2012/12042-7, 2013-17368-0, PROADI_ SUS Project nº 25000.180664/2011-35).
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The authors declare that they have no conflict of interest.
Ethical approval
All experiments were carried out in accordance with the ethics committee of the Hospital das Clinicas, University of São Paulo, Brazil (protocol number 3759/12/015), approved the study protocol.
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Figure S1
PCA scores plot based on the GC/MS spectra of serum samples obtained from diabetic patients treated and diabetic patients not treated with hypoglycemic medication. (PDF 20 kb)
Figure S2
Samples first principal component scores distributed by the day of analysis. The red points represent the diabetic samples subject. (TIFF 1346 kb)
Figure S3
Metabolites concentration before (A) and after (B) LOESS normalization according to injection order and day of analysis. (TIFF 2194 kb)
Figure S4
Representativity of significant metabolites found by polygenic analysis. The blue side represent diabetic group, and the red side represent the non-diabetic group. (TIFF 1426 kb)
Table S1
Main biological functions disturbed relate with metabolites found by PLS-DA to diabetic, pre-diabetic and non diabetic participants. In #molecules column is the number of metabolites present in each pathway. (DOCX 15 kb)
Table S2
Heritability (h2) estimates (in percentage) for each metabolite, unadjusted (Model 1), adjusted for age and sex (Model 2), and adjusted for age, sex and T2DM (Model 3). (XLSX 15 kb)
Table S3
Main biological functions disturbed by proximal metabolites. In #molecules column is the number of metabolites present in each pathway. (DOCX 15 kb)
Table S4
Main biological functions related to distal metabolites. In #molecules column is the number of metabolites present in each pathway. (DOCX 15 kb)
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Padilha, K., Venturini, G., de Farias Pires, T. et al. Serum metabolomics profile of type 2 diabetes mellitus in a Brazilian rural population. Metabolomics 12, 156 (2016). https://doi.org/10.1007/s11306-016-1107-5
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DOI: https://doi.org/10.1007/s11306-016-1107-5