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Mediterranean diet reduces senescence-associated stress in endothelial cells

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Abstract

This paper aims to study the effects of the oxidative stress induced by quality and quantity of dietary fat on cellular senescence. Twenty elderly subjects consumed three diets, each for 4 weeks: a saturated fatty acid diet (SFA), a low-fat and high-carbohydrate diet (CHO-ALA), and a Mediterranean diet (MedDiet) enriched in monounsaturated fatty acid following a randomized crossover design. For each diet, we investigated intracellular reactive oxidative species (ROS), cellular apoptosis and telomere length in human umbilical endothelial cells incubated with serum from each patient. MedDiet induced lower intracellular ROS production, cellular apoptosis, and percentage of cell with telomere shortening, compared with the baseline and with SFA and CHO-ALA diets. Dietary fat modulates the oxidative stress in human endothelial cells. MedDiet protects these cells from oxidative stress, prevents cellular senescence and reduces cellular apoptosis.

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Abbreviations

MUFA:

Monounsaturated fatty acid

PUFA:

Polyunsaturated fatty acid

ROS:

Reactive oxygen species

SFA diet:

Saturated fatty acid-rich diet

CHO-ALA diet:

Low-fat, high-carbohydrate diet enriched with n-3 PUFA

MedDiet:

Mediterranean diet (MedDiet) enriched with monounsaturated fatty acid

HUVEC:

Human umbilical endothelial cells

TNF-α:

Tumor necrosis factor alpha

FITC:

Fluorescein isothiocyanate

FCS:

Fetal calf serum

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Acknowledgments

This study was funded partly by research grants from the Spanish Ministry of Science and Innovation (AGL 2004–07907, AGL2006-01979, AGL2009-12270 to JL-M, and FIS PI10/01041 to PP-M), Consejería de Economía, Innovación y Ciencia, Proyectos de Investigación de Excelencia, Junta de Andalucía (P06-CTS-01425 to JL-M, CTS5015 to FP-J); Consejería de Salud, Junta de Andalucía (06/128, 07/43, and PI0193/09 to JL-M, 06/129 to FP-J, 06/127 to CM, 0118/08 to FF-J, PI-0252/09 to JD-L, and PI-0058/10 to PP-M, PI-JA0235/2009 to JC); FIS PI08/1038, PS09/00836 to JC, Consejería de Salud, Junta de Andalucía. J. Carracedo was supported by a contract from Fundación de Investigaciones Biomédicas de Córdoba (FIBICO, Programa I3SNS, Línea de estabilización de investigadores del Instituto de Salud Carlos III/Fundación Progreso y Salud). Fondo Europeo de Desarrollo Regional (FEDER). The CIBEROBN is an initiative of the Instituto de Salud Carlos III, Madrid, Spain.

The authors’ responsibilities were as follows—JLM designed and conducted the research; JDL, PPM, and JLM provided materials or participants; CM, RR, JC, JC, PPM, AGR, NDC, FMGM, EMYS, and CCT collected and collated the data; CM, JDL, JLM, RR, and JC analyzed data; CM wrote the paper; JDL, PPM, FT, and MMM provided considerable advice and support in reviewing the drafting of the paper; JLM and FP-J had primary responsibility for the final content. All the authors read and approved the final manuscript. None of the authors has any conflict of interest that could affect the performance of the work or the interpretation of the data.

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Authors and Affiliations

  1. Lipids and Atherosclerosis Unit, Córdoba, Spain

    Carmen Marin, Javier Delgado-Lista, Pablo Perez-Martinez, Francisco Miguel Gutierrez-Mariscal, Antonio Garcia-Rios, Nieves Delgado-Casado, Cristina Cruz-Teno, Elena Maria Yubero-Serrano, Francisco Perez-Jimenez & Jose Lopez-Miranda

  2. Instituto Maimonides de Investigación Biomedica de Cordoba/Hospital Universitario Reina Sofía/Universidad de Córdoba and CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Cordoba, Spain

    Carmen Marin, Javier Delgado-Lista, Pablo Perez-Martinez, Francisco Miguel Gutierrez-Mariscal, Antonio Garcia-Rios, Nieves Delgado-Casado, Cristina Cruz-Teno, Elena Maria Yubero-Serrano, Maria del Mar Malagon, Francisco Perez-Jimenez & Jose Lopez-Miranda

  3. Instituto Maimonides de Investigación Biomedica de Cordoba/Hospital Universitario Reina Sofía/Universidad de Córdoba/Fundación para la Investigación Biomédica de Córdoba, Córdoba, Spain

    Rafael Ramirez & Julia Carracedo

  4. Clinical Analysis Service/Hospital Universitario Reina Sofía, Córdoba, Spain

    Javier Caballero

  5. Servicio de Endocrinología y Nutrición, Hospital Clínico Virgen de la Victoria, CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Málaga, Spain

    Francisco Tinahones

  6. Department of Cell Biology, Physiology and Immunology, CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Córdoba, Spain

    Maria del Mar Malagon

  7. Reina Sofia University Hospital, Lipids and Atherosclerosis Research Unit, University of Cordoba, Avd. Menendez Pidal s/n., 14004, Córdoba, Spain

    Jose Lopez-Miranda

Authors
  1. Carmen Marin
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  2. Javier Delgado-Lista
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  3. Rafael Ramirez
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  8. Antonio Garcia-Rios
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  11. Elena Maria Yubero-Serrano
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  12. Francisco Tinahones
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  13. Maria del Mar Malagon
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  14. Francisco Perez-Jimenez
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  15. Jose Lopez-Miranda
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Corresponding author

Correspondence to Jose Lopez-Miranda.

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Marin, C., Delgado-Lista, J., Ramirez, R. et al. Mediterranean diet reduces senescence-associated stress in endothelial cells. AGE 34, 1309–1316 (2012). https://doi.org/10.1007/s11357-011-9305-6

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  • DOI: https://doi.org/10.1007/s11357-011-9305-6

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