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Insulin-like growth factor 1 improves the efficacy of mesenchymal stem cells transplantation in a rat model of myocardial infarction

  • Published:
Journal of Biomedical Science

Abstract

Background Previous study demonstrated the improvement of cardiac function was proportional to the number of cells implanted. Therefore, increasing cell survival in the infarcted myocardium might contribute to the improvement of the functional benefit of cell transplantation. Methods and results MSCs were treated with IGF-1 in vitro and infused into the acute myocardial infarction rats via the tail vein. After treatment of MSCs with IGF-1 for 48 h, flow cytometric analysis showed marked enhancement of expression of CXCR4 in the cell surface. After 4 weeks of transplantation, we found 1) a greater number of engrafted MSCs arrived and survived in the peri-infarct region; 2) TnT protein expression and capillary density were enhanced; 3) LV cavitary dilation, transmural infarct thinning, deposition of total collagen in the peri-infarct region and cardiac dysfunction were attenuated. Conclusion 1) IGF-1 treatment has time-dependent and dose-dependent effects on CXCR4 expression in MSCs in vitro. 2) IGF-1 improves the efficacy of MSCs transplantation in a rat model of myocardial infarction mainly via enhancement of the number of cells attracted into the infarcted heart. These findings provide a novel stem cell therapeutic avenue against ischemic heart disease.

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Acknowledgements

We thank Xi Wang, Ping Hu, Yanhong Tang (Department of Cardiology, Renmin Hospital, Wuhan University School of Medicine, Wuhan, China) for great assistance. This project was supported by Department of Health of Hubei province (JX3B48), Department of Education of Hubei province for excellent youth science and tech group (No T200606), Department of Technology of Hubei province (2005AA301C38–2) and Xianning college (KY0565).

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Authors and Affiliations

  1. Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan University School of Medicine, 238 JieFang Road, Wuchang, Wuhan, 430060, China

    Jun Guo, Guosheng Lin, Honggang Chu & Mingyan Hu

  2. Cardiovascular Research Institute, Xianning College, Xianning, Hubei, P.R. China

    Cuiyu Bao

  3. Department of Dermatology, First Hospital of Wuhan, Wuhan, Hubei, P.R. China

    Zhimin Hu

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  1. Jun Guo
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Correspondence to Guosheng Lin.

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Guo, J., Lin, G., Bao, C. et al. Insulin-like growth factor 1 improves the efficacy of mesenchymal stem cells transplantation in a rat model of myocardial infarction. J Biomed Sci 15, 89–97 (2008). https://doi.org/10.1007/s11373-007-9207-x

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  • DOI: https://doi.org/10.1007/s11373-007-9207-x

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