Summary
In this study, the colonization and distribution of Helicobacter pylori (Hp) in patients with chronic gastric diseases were investigated and the relationship between the periodontal initial treatment and presence of Hp in oral cavity was examined to better understand the connection between Hp infection and chronic diseases. Primers for PCR amplification were designed according to ureC gene and cagA genes of Hp. Specimens were harvested from different sites of 96 patients with chronic gastric diseases and the specimens of dental plaques, gargles and dorsal mucosa were tested for Hp. The 96 patients were treated by bismuth triple therapy and among them, 52 subjects were additionally given periodontal initial therapy. The eradication rate of gastric Hp and oral Hp detection rate were determined 4 weeks and 1 year after the treatment. The results showed that the detection rates of oral specimens were in the order of dental plaques (82.3%), gargles (51.1%) and scrapings of dorsal mucosa of tongue (37.5%). One year after bismuth triple therapy or the triple therapy in combination with periodontal initial treatment, the eradication rate of gastric Hp was significantly higher in the combination treatment group than in group treated by the triple therapy alone (62.8% vs. 32.4%, P<0. 05). Moreover, the Hp detection rate was significantly lower in the combination group than in the group treated only with the triple therapy. We are led to conclude that Hp is present at various parts of oral cavity, oral Hp might be an important source of gastric Hp and the triple therapy plus periodontal initial treatment can enhance the long-term eradication rate of gastric Hp in patient with both chronic gastric diseases and chronic periodontitis.
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References
Krajden S, Fuksa M, Anderson J, et al. Examination of human stomach biopsies saliva, and dental plaque for Campylobacter pylori. J Clin Microbiol, 1989,27(6): 1397–1398
Silva DG, Tinoco EM, Rocha AM, et al. Helicobacter pylori transiently in the mouth may participate in the transmission of infection. Mem Inst Oswaldo Cruz, 2010, 105(5):657–660
Eskandari A, Mahmoudpour A, Abolfazli N, et al. Detection of Helico-bacter pylori using PCR in dental plaque of patients with and without gastritis. Med Oral Patol Oral Cir Bucal, 2010,15(1):e28–e31
Bürgers R, Schneider-Brachert W, Reischl U, et al. Helicobacter pylori in human oral cavity and stomach. Eur J Oral Sci, 2008,116(4):297–304
Bickley J, Owen RJ, Fraser AG, et al. Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacter pylori in gastric biopsy samples and dental plaque. J Med Microbiol, 1993, 39:338–344
Lage AP, Godpoid E, Fauconnier A, et al. Diagnosis of Helicobacter pylori infection by PCR: Comparison with other invasive techniques and detection of cag A gene in gastric biopsy specimens. J Clin Microbiol, 1995,9:2752
Silva DG, Tinoco EM, Rocha GA, et al. Helicobacter pylori transiently in the mouth may participate in the transmission of infection. Mem Inst Oswaldo Cruz, 2010,105(5):657–660
Morales-Espinosa R, Fernandez-Presas A, Gonzalez-Valencia G, et al. Helicobacter pylori in the oral cavity is associated with gastroesophageal disease. Oral Microbiol Immunol, 2009,24(6):464–468
Silva DG, Stevens RH, Macedo JM, et al. Detection of cytotoxin genotypes of Helicobacter pylori in stomach, saliva and dental plaque. Arch Oral Biol, 2009,54(7): 684–688
Fernández-Tilapa G, Axinecuilteco-Hilera J, Giono-Cerezo S, et al. VacA genotypes in oral cavity and Helicobacter pylori seropositivity among adults without dyspepsia. Med Oral Patol Oral Cir Bucal, 2011,16(2):e175–e180
Olsson K, Wadstrom T, Tyszkiewicz T. Helicobacter pylori in dental plaques. Lancet, 1993,34(1):956–957
Gao J. The relationship between Helicobacter pylori in oral cavity and the Hp infection in stomach from chronic gastritis patients. J Taishanmedical College (Chinese), 2010,31(4):252–254
Hu W, Cao C, Meng H, et al. Detection and analysis of Helicobacter pylori in oral cavity and stomach from chronic gastritis patients. Zhonghua Yi Xue Za Zhi (Chinese), 2002,82(15):1037–1041
Song Q, Lange T, Spahr A, et al. Characterisitic distribution pattern of Helicobacter pylori in dental plaque and saliva detected with nested PCR. J Med Microbiol, 2000,49(4): 349–353
Sheu BS, Cheng HC, Yang YJ, et al. The presence of dentaldisease can be a risk factor for recurrent Helicobacter pylori infection after eradication therapy: a 3-year follow-up. Endoscopy, 2007,39(11):942–947
Kignel S, de Almeida Pina F, Andre EA, et al. Occurrence of Helicobacter pylori in dental plaque and saliva of dyspeptic patients. Oral Dis, 2005,11(1):17–21
Jia CL, Jiang GS, Li CH, et al. Effect of dental plaque control on infection of Helicobacter pylori in gastric mucosa. J Periodontol, 2009,80(10):1606–1609
Velin D, Michetti P. Advances in vaccination against Helicobacter pylori. Expert Rev Gastroenterol Hepatol, 2010,4(2):157–166
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This project was supported by a grant from the Bureau of Science and Technology under Tai’an Municipal Government (No. TKJ2002-9).
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Gao, J., Li, Y., Wang, Q. et al. Correlation between distribution of Helicobacter Pylori in oral cavity and chronic stomach conditions. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 409–412 (2011). https://doi.org/10.1007/s11596-011-0391-6
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DOI: https://doi.org/10.1007/s11596-011-0391-6