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Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid

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Abstract

Objective

To observe the regulation of Youguiyin (YGY, 右归饮) on the gene expression profile of the rat with steroid-induced femoral head necrosis (sFHN), for the sake of investigating its molecular mechanism of sFHN prevention and treatment.

Methods

All the 30 rats were randomly divided into three groups, the normal control group (A), the model control group (B), and the YGY treated group (C), 10 in each group. After rats in Groups B and C were being made into FHN models with steroid injection, they received a daily intragastric administration of saline and YGY respectively in equal volume for a total of 6 weeks, while to the unmodeled normal rats in Group A, saline was administered instead. The rats were sacrificed at the terminal of administration; their mRNA from femoral head tissue was extracted and prepared to cDNA probe through inverse transcription for detecting gene expression profile by microarray, outcomes of which was passing fluorescence quantitative PCR verification, and the differential expressed genes were analyzed adopting gene ontology (GO) method.

Results

Compared with Group A, the numbers of differential genes found in Groups B and C were 190 and 92, respectively, but the changing trend in the two groups was opposite, mainly manifested as down-regulating in Group B/Group A (GB/GA) and up-regulating in Group C/Group B (GC/GB). The analysis showed that these differential genes were mainly assigned to cell apoptosis, signal transduction, metabolism, cell proliferation and differentiation, cell cycle, blood coagulation, antioxidant activity, etc.

Conclusions

sFHN was regulated by various genes; the regulation of YGY on expressions of these genes and the intra/extra-cellular signaling processes was possibly the molecular mechanism of YGY for preventing/treating sFHN. This study gave an explanation to the effectiveness of Chinese medicine in preventing/treating FHN from aspects of gene expression and enriched the Chinese medicine theory of “Kidney (Shen) governing bones”.

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Authors and Affiliations

  1. Zhejiang Traditional Chinese Medicine University, Hangzhou, 310053, China

    Cheng-liang Wu  (吴承亮), Qiang Mao  (毛 强), Hong-ting Jin  (金红婷) & Lu-wei Xiao  (肖鲁伟)

  2. Zhejiang Provincial Institute of Ortopaedics and Traumatology, Hangzhou, 310053, China

    Cheng-liang Wu  (吴承亮), Qiang Mao  (毛 强), Hong-ting Jin  (金红婷), Lu-wei Xiao  (肖鲁伟) & Pei-jian Tong  (童培建)

  3. Ortopaedics and Traumatology Department of Zhejiang Provincial Traditional Chiese Medicine Hospital, the First Affiliated Hospital of Zhejiang Traditional Chinese Medicine University, Hangzhou, 310006, China

    Pei-jian Tong  (童培建)

Authors
  1. Cheng-liang Wu  (吴承亮)
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  2. Qiang Mao  (毛 强)
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  3. Hong-ting Jin  (金红婷)
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  4. Lu-wei Xiao  (肖鲁伟)
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  5. Pei-jian Tong  (童培建)
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Corresponding author

Correspondence to Pei-jian Tong  (童培建).

Additional information

Supported by the National Natural Science Foundation of China (No. 30672702, No. 81072820 and No. 30873276) and Key Program of Traditional Chinese Medicine of Zhejiang Province (No. 2005Z003)

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Wu, Cl., Mao, Q., Jin, Ht. et al. Study on the effect of Youguiyin (右归饮) on regulating gene expression profile of the rats with femoral head necrosis induced by steroid. Chin. J. Integr. Med. 18, 269–275 (2012). https://doi.org/10.1007/s11655-012-1051-9

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  • DOI: https://doi.org/10.1007/s11655-012-1051-9

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