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Detection and Clinical Significance of CD44v6 and Integrin-β1 in Pancreatic Cancer Patients using a Triplex Real-Time RT-PCR Assay

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Abstract

The cell adhesion molecules CD44v6 and integrin-β1 are associated with the progression and metastasis of cancer. A novel triplex real-time reverse transcription polymerase chain reaction (qRT-PCR) assay was developed to quantify CD44v6 and integrin-β1 gene expression in peripheral blood mononuclear cells from 30 pancreatic cancer (PC) patients and 12 healthy individuals. The standard curve of the triplex qRT-PCR was constructed by optimizing the reaction condition and the amplification efficiency was 102.5, 101.1, and 100.6 % for CD44v6, integrin-β1 and endogenous gene (β-actin) amplification. Nonspecific bands were not observed from the triplex qRT-PCR amplification and the detection limit of this assay was 100 copies. Expression levels of CD44v6 and integrin-β1 gene were significantly lower in healthy individuals than PC patients (P < 0.05). CD44v6 and integrin-β1 gene expression were not associated with the sex, age, and tumor position in PC (P > 0.05). CD44v6 gene expression was significantly associated with clinical stage, liver metastasis, and tumor size (P < 0.05). Integrin-β1 gene expression was significantly associated with clinical stage and liver metastasis (P < 0.05). This triplex qRT-PCR assay may provide a useful tool for diagnosis, prognosis, and therapeutic evaluation in PC.

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Acknowledgments

This work was supported by the Fuda Cancer Hospital of Guangzhou in China and conducted at the Stem Cell Research Group of Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences. We would like to express appreciation to Xiaopeng Liu, Zhiju Zhao, Chengqian Feng, and Mengtian Liao for processing clinical samples.

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Authors and Affiliations

  1. Department of Oncology, The GIBH Affiliated Fuda Hospital, Chinese Academy of Sciences, 91 Jude Zhong Road, Chigang, Guangzhou, China

    Gang Zhou, David Chiu, Lizhi Niu & Lihua He

  2. Stem Cell Research Group, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kai Yuan Avenue, Science Park, Guangzhou, China

    Dajiang Qin, Jinlei Cai & Wenhao Huang

  3. The GIBH Affiliated Fuda Hospital, Chinese Academy of Sciences, 91 Jude Zhong Road, Chigang, Guangzhou, China

    Kecheng Xu

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  1. Gang Zhou
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  2. David Chiu
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  3. Dajiang Qin
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  4. Lizhi Niu
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Correspondence to Kecheng Xu.

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Zhou, G., Chiu, D., Qin, D. et al. Detection and Clinical Significance of CD44v6 and Integrin-β1 in Pancreatic Cancer Patients using a Triplex Real-Time RT-PCR Assay. Appl Biochem Biotechnol 167, 2257–2268 (2012). https://doi.org/10.1007/s12010-012-9752-2

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  • DOI: https://doi.org/10.1007/s12010-012-9752-2

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