Abstract
Minerals are inorganic compounds that are essential to the support of a variety of biological functions. Understanding the range and variability of the content of these minerals in biological samples can provide insight into the relationships between mineral content and the health of individuals. In particular, abnormal mineral content may serve as an indicator of illness. The development of robust, reliable analytical methods for the determination of the mineral content of biological samples is essential to developing biological models for understanding the relationship between minerals and illnesses. This paper describes a method for the analysis of the mineral content of small volumes of serum and whole blood samples from healthy individuals. Interday and intraday precision for the mineral content of the blood (250 μL) and serum (250 μL) samples was measured for eight essential minerals—sodium (Na), calcium (Ca), magnesium (Mg), potassium (K), iron (Fe), zinc (Zn), copper (Cu), and selenium (Se)—by plasma spectrometric methods and ranged from 0.635 to 10.1 % relative standard deviation (RSD) for serum and 0.348–5.98 % for whole blood. A comparison of the determined ranges for ten serum samples and six whole blood samples provided good agreement with literature reference ranges. The results demonstrate that the digestion and analysis methods can be used to reliably measure the content of these minerals and potentially of other minerals.
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Abbreviations
- ICP-MS:
-
Inductively coupled plasma mass spectrometry
- ICP-OES:
-
Inductively coupled plasma optical emission spectroscopy
- NIST:
-
National Institute of Standards and Technology
- ELOQ:
-
Estimated limit of quantitation
- LOD:
-
Limit of detection
- KED:
-
Kinetic energy discrimination
- SRM:
-
Standard reference material
- SF-MS:
-
Sector field mass spectrometry
References
Frausto da Silva JJR, Williams RJP (2001) The biological chemistry of the elements, 2nd edn. Oxford University Press, Oxford
Coffey AJ, Durkie M, Hague S, McLay K, Emmerson J, Lo C, Klaffke S, Joyce CJ, Dhawan A, Hadzic N, Mieli-Vergani G, Kirk R, Allen KE, Nicholl D, Wong S, Griffiths W, Smithson S, Giffin N, Taha A, Connolly S, Gillett GT, Tanner S, Bonham J, Sharrack B, Palotie A, Rattray M, Dalton A, Bandmann O (2013) A genetic study of Wilson's disease in the United Kingdom. Brain 136(Part 5):1476–1487. doi:10.1093/brain/awt035
Mainous AG III, Wright RU, Hulihan MM, Twal WO, McLaren CE, Diaz VA, McLaren GD, Argraves WS, Grant AM (2014) Elevated transferrin saturation, health-related quality of life and telomere length. Biometals 27(1):135–141. doi:10.1007/s10534-013-9693-4
Hu LG, He B, Wang YC, Jiang GB, Sun HZ (2013) Metallomics in environmental and health related research: current status and perspectives. Chin Sci Bull 58(2):169–176. doi:10.1007/s11434-012-5496-1
Wang B, Feng WY, Zhao YL, Chai ZF (2013) Metallomics insights for in vivo studies of metal based nanomaterials. Metallomics 5(7):793–803. doi:10.1039/c3mt00093a
Sussulini A, Becker JS (2011) Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages and limitations. Metallomics 3(12):1271–1279. doi:10.1039/c1mt00116g
Mounicou S, Szpunar J, Lobinski R (2009) Metallomics: the concept and methodology. Chem Soc Rev 38(4):1119–1138. doi:10.1039/b713633c
Yasuda H, Yonashiro T, Yoshida K, Ishii T, Tsutsui T (2006) Relationship between body mass index and minerals in male Japanese adults. Biomed Res Trace Elem 17:316–321
Liu F, Gan PP, Wu HN, Woo WS, Ong ES, Li SFY (2012) A combination of metabolomics and metallomics studies of urine and serum from hypercholesterolaemic rats after berberine injection. Anal Bioanal Chem 403(3):847–856. doi:10.1007/s00216-012-5923-9
Easter RN, Chan QL, Lai B, Ritman EL, Caruso JA, Qin ZY (2010) Vascular metallomics: copper in the vasculature. Vasc Med 15(1):61–69. doi:10.1177/1358863x09346656
Popovic D, Bozic T, Stevanovic J, Frontasyeva M, Todorovic D, Ajtic J, Jokic VS (2010) Concentration of trace elements in blood and feed of homebred animals in Southern Serbia. Environ Sci Pollut Res 17:1119–1128
Yoshinaga J, Li JZ, Suzuki T, Karita K, Abe M, Fujii H, Mishina J, Morita M (1991) Trace elements in human transitory milk: variation caused by biological attributes of mother and infant. Biol Trace Elem Res 31:159–170
Karadas S, Sayin R, Aslan M, Gonullu H, Kati C, Dursun R, Duran L, Gonullu E, Demir H (2014) Serum levels of trace elements and heavy metals in patients with acute hemorrhagic stroke. J Membr Biol 247:175–180
Taylor A, Day MP, Hill S, Marshall J, Patriarca M, White M (2014) Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages. J Anal Atom Spectrom 29(3):386–426. doi:10.1039/c4ja90001d
Olmedo P, Pla A, Hernandez AF, Lopez-Guarnido O, Rodrigo L, Gil F (2010) Validation of a method to quantify chromium, cadmium, manganese, nickel and lead in human whole blood, urine, saliva and hair samples by electrothermal atomic absorption spectrometry. Anal Chim Acta 659(1–2):60–67. doi:10.1016/j.aca.2009.11.056
Zaksas NP, Gerasimov VA, Nevinsky GA (2010) Simultaneous determination of Fe, P, Ca, Mg, Zn, and Cu in whole blood by two-jet plasma atomic emission spectrometry. Talanta 80(5):2187–2190. doi:10.1016/j.talanta.2009.10.046
Ivanenko NB, Ivanenko AA, Solovyev ND, Zeimal AE, Navolotskii DV, Drobyshev EJ (2013) Biomonitoring of 20 trace elements in blood and urine of occupationally exposed workers by sector field inductively coupled plasma mass spectrometry. Talanta 116:764–769. doi:10.1016/j.talanta.2013.07.079
Kolachi NF, Kazi TG, Afridi HI, Kazi N, Khan S, Kandhro GA, Shah AQ, Baig JA, Wadhwa SK, Shah F, Jamali MK, Arain MB (2011) Status of toxic metals in biological samples of diabetic mothers and their neonates. Biol Trace Elem Res 143(1):196–212. doi:10.1007/s12011-010-8879-7
Afridi HI, Kazi TG, Kazi N, Jamali MK, Arain MB, Jalbani N, Sarfaraz RA, Shah A, Kandhro GA, Shah AQ, Baig JA (2008) Potassium, calcium, magnesium, and sodium levels in biological samples of hypertensive and nonhypertensive diabetes mellitus patients. Biol Trace Elem Res 124(3):206–224. doi:10.1007/s12011-008-8142-7
Bocca B, Alimonti A, Petrucci F, Violante N, Sancesario G, Forte G, Senofonte O (2004) Quantification of trace elements by sector field inductively coupled plasma mass spectrometry in urine, serum, blood and cerebrospinal fluid of patients with Parkinson's disease. Spectrochim Acta B 59(4):559–566. doi:10.1016/j.sab.2004.02.007
Bocca B, Alimonti A, Forte G, Petrucci F, Pirola C, Senofonte O, Violante N (2003) High-throughput microwave-digestion procedures to monitor neurotoxic elements in body fluids by means of inductively coupled plasma mass spectrometry. Anal Bioanal Chem 377(1):65–70. doi:10.1007/s00216-003-2029-4
Iyengar V, Woittiez J (1988) Trace elements in human clinical specimens: evaluation of literature data to identify reference values. Clin Chem 34:474–481
Case CP, Ellis L, Turner JC, Fairman B (2001) Development of a routine method for the determination of trace metals in whole blood by magnetic sector inductively coupled plasma mass spectrometry with particular relevance to patients with total hip and knee arthroplasty. Clin Chem 47(2):275–280
D'Ilio S, Violante N, Majorani C, Petrucci F (2011) Dynamic reaction cell ICP-MS for determination of total As, Cr, Se and V in complex matrices: still a challenge? A review. Anal Chim Acta 698(1–2):6–13. doi:10.1016/j.aca.2011.04.052
Tokuda E, Okawa E, Watanabe S, S-i O (2014) Overexpression of metallothionein-I, a copper-regulating protein, attenuates intracellular copper dyshomeostasis and extends lifespan in a mouse model of amyotrophic lateral sclerosis caused by mutant superoxide dismutase-1. Hum Mol Genet 23(5):1271–1285. doi:10.1093/hmg/ddt517
Krachler M, Rossipal E, Micetic-Turk D (1999) Concentrations of trace elements in sera of newborns, young infants, and adults. Biol Trace Elem Res 68(2):121–136. doi:10.1007/bf02784401
Henry JB (1974) Clinical diagnosis and management by laboratory methods, vol 1. Sanders, Philadelphia
Clark NA, Teschke K, Rideout K, Copes R (2007) Trace element levels in adults from the west coast of Canada and associations with age, gender, diet, activities, and levels of other trace elements. Chemosphere 70(1):155–164. doi:10.1016/j.chemosphere.2007.06.038
Acknowledgments
This project was supported by the NIH Eastern Regional Metabolomics Resource Core (NIH Common Fund Grant 1U24DK097193; PI Susan Sumner) and the NIH Clinical and Translational Sciences Award (NCATS Grant UL1TR00111; PI Marshall Runge). Mr. Glenn Ross is appreciated for providing logistical support for the development of the experimental protocol.
Ethical Statement
This manuscript does not contain samples that were obtained from clinical studies and no personally identifiable patient data are included. Sample collection procedures followed the Helsinki Declaration guidelines regarding informed consent of human volunteers. The authors declare that they have no conflict of interest.
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Harrington, J.M., Young, D.J., Essader, A.S. et al. Analysis of Human Serum and Whole Blood for Mineral Content by ICP-MS and ICP-OES: Development of a Mineralomics Method. Biol Trace Elem Res 160, 132–142 (2014). https://doi.org/10.1007/s12011-014-0033-5
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DOI: https://doi.org/10.1007/s12011-014-0033-5