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Erschienen in: Cardiovascular Toxicology 3/2015

01.07.2015

Protective Effect of Apigenin on Ischemia/Reperfusion Injury of the Isolated Rat Heart

verfasst von: Jing Hu, Zilin Li, Li-ting Xu, Ai-jun Sun, Xiao-yan Fu, Li Zhang, Lin-lin Jing, An-dong Lu, Yi-fei Dong, Zheng-ping Jia

Erschienen in: Cardiovascular Toxicology | Ausgabe 3/2015

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Abstract

Apigenin (Api), a mainly bioactive component of Apium graveolens L. var. dulce DC. (a traditional Chinese medicinal herb), possesses a wide range of biological activities, including antioxidant effects. It also has been shown to associate with lower prevalence of cardiovascular diseases, but its mechanisms of action remain unclear. The aim of the present study is to investigate the role of Api in isolated rat heart model of ischemia/reperfusion (I/R). Langendorff-perfused isolated rat hearts were used in our study. Api was added to the perfusate before ischemia and during reperfusion in the isolated pulsed rat heart exposed to 30-min ischemia followed by 50-min reperfusion. The treatment with Api conferred a cardioprotective effect, and the treated hearts demonstrated an improved ischemic cardiac functional recovery, a decreased myocardial infarct size, a reduced activities of creatine kinase isoenzyme and lactate dehydrogenase in the coronary flow, a reduced number of apoptotic cardiomyocytes, a reduced activity of caspase-3, up-regulation of the anti-apoptotic protein Bcl-2 and down-regulation of the pro-apoptotic protein Bax. In addition, Api inhibited the phosphorylation of p38 MAPKS during I/R. In conclusion, these observations provide preliminary evidence that Api can protect cardiomyocytes from I-/R-induced injury, at least partially, through the inhibition of p38 MAPKS signaling pathway.
Literatur
1.
Zurück zum Zitat Sharma, H., Kanwal, R., Bhaskaran, N., & Gupta, S. (2014). Plant flavone apigenin binds to nucleic acid bases and reduces oxidative DNA damage in prostate epithelial cells. PLoS ONE, 9, e91588.PubMedCentralPubMedCrossRef Sharma, H., Kanwal, R., Bhaskaran, N., & Gupta, S. (2014). Plant flavone apigenin binds to nucleic acid bases and reduces oxidative DNA damage in prostate epithelial cells. PLoS ONE, 9, e91588.PubMedCentralPubMedCrossRef
2.
Zurück zum Zitat Lu, X. Y., Sun, D. L., Chen, Z. J., Chen, T., Li, L. P., Xu, Z. H., et al. (2010). Relative contribution of small and large intestine to deglycosylation and absorption of flavonoids from Chrysanthemum morifolium extract. Journal of Agriculture and Food Chemistry, 58, 10661–10667.CrossRef Lu, X. Y., Sun, D. L., Chen, Z. J., Chen, T., Li, L. P., Xu, Z. H., et al. (2010). Relative contribution of small and large intestine to deglycosylation and absorption of flavonoids from Chrysanthemum morifolium extract. Journal of Agriculture and Food Chemistry, 58, 10661–10667.CrossRef
3.
Zurück zum Zitat Beara, I. N., Lesjak, M. M., Jovin, E. D., Balog, K. J., Anackov, G. T., Orcic, D. Z., et al. (2009). Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. Journal of Agriculture and Food Chemistry, 57, 9268–9273.CrossRef Beara, I. N., Lesjak, M. M., Jovin, E. D., Balog, K. J., Anackov, G. T., Orcic, D. Z., et al. (2009). Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. Journal of Agriculture and Food Chemistry, 57, 9268–9273.CrossRef
4.
Zurück zum Zitat Kanazawa, K., Uehara, M., Yanagitani, H., & Hashimoto, T. (2006). Bioavailable flavonoids to suppress the formation of 8-OHdG in HepG2 cells. Archives of Biochemistry and Biophysics, 455, 197–203.PubMedCrossRef Kanazawa, K., Uehara, M., Yanagitani, H., & Hashimoto, T. (2006). Bioavailable flavonoids to suppress the formation of 8-OHdG in HepG2 cells. Archives of Biochemistry and Biophysics, 455, 197–203.PubMedCrossRef
5.
Zurück zum Zitat Zhang, Y. H., Park, Y. S., Kim, T. J., Fang, L. H., Ahn, H. Y., Hong, J. T., et al. (2000). Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin. General Pharmacology, 35, 341–347.PubMedCrossRef Zhang, Y. H., Park, Y. S., Kim, T. J., Fang, L. H., Ahn, H. Y., Hong, J. T., et al. (2000). Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin. General Pharmacology, 35, 341–347.PubMedCrossRef
6.
Zurück zum Zitat Basile, A., Giordano, S., Lopez-Saez, J. A., & Cobianchi, R. C. (1999). Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry, 52, 1479–1482.PubMedCrossRef Basile, A., Giordano, S., Lopez-Saez, J. A., & Cobianchi, R. C. (1999). Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry, 52, 1479–1482.PubMedCrossRef
7.
Zurück zum Zitat Gupta, S., Afaq, F., & Mukhtar, H. (2002). Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells. Oncogene, 21, 3727–3738.PubMedCrossRef Gupta, S., Afaq, F., & Mukhtar, H. (2002). Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells. Oncogene, 21, 3727–3738.PubMedCrossRef
8.
Zurück zum Zitat Lindenmeyer, F., Li, H., Menashi, S., Soria, C., & Lu, H. (2001). Apigenin acts on the tumor cell invasion process and regulates protease production. Nutrition and Cancer, 39, 139–147.PubMedCrossRef Lindenmeyer, F., Li, H., Menashi, S., Soria, C., & Lu, H. (2001). Apigenin acts on the tumor cell invasion process and regulates protease production. Nutrition and Cancer, 39, 139–147.PubMedCrossRef
9.
Zurück zum Zitat Jin, B. H., Qian, L. B., Chen, S., Li, J., Wang, H. P., Bruce, I. C., et al. (2009). Apigenin protects endothelium-dependent relaxation of rat aorta against oxidative stress. European Journal of Pharmacology, 616, 200–205.PubMedCrossRef Jin, B. H., Qian, L. B., Chen, S., Li, J., Wang, H. P., Bruce, I. C., et al. (2009). Apigenin protects endothelium-dependent relaxation of rat aorta against oxidative stress. European Journal of Pharmacology, 616, 200–205.PubMedCrossRef
10.
Zurück zum Zitat Meyer, H., Bolarinwa, A., Wolfram, G., & Linseisen, J. (2006). Bioavailability of apigenin from apiin-rich parsley in humans. Annals of Nutrition and Metabolism, 50, 167–172.PubMedCrossRef Meyer, H., Bolarinwa, A., Wolfram, G., & Linseisen, J. (2006). Bioavailability of apigenin from apiin-rich parsley in humans. Annals of Nutrition and Metabolism, 50, 167–172.PubMedCrossRef
11.
Zurück zum Zitat Bellosta, S., Bogani, P., Canavesi, M., Galli, C., & Visioli, F. (2008). Mediterranean diet and cardioprotection: Wild artichoke inhibits metalloproteinase 9. Molecular Nutrition and Food Research, 52, 1147–1152.PubMedCrossRef Bellosta, S., Bogani, P., Canavesi, M., Galli, C., & Visioli, F. (2008). Mediterranean diet and cardioprotection: Wild artichoke inhibits metalloproteinase 9. Molecular Nutrition and Food Research, 52, 1147–1152.PubMedCrossRef
12.
Zurück zum Zitat Jeong, C. W., Yoo, K. Y., Lee, S. H., Jeong, H. J., Lee, C. S., & Kim, S. J. (2012). Curcumin protects against regional myocardial ischemia/reperfusion injury through activation of RISK/GSK-3beta and inhibition of p38 MAPK and JNK. Journal of Cardiovascular Pharmacology and Therapeutics, 17, 387–394.PubMedCrossRef Jeong, C. W., Yoo, K. Y., Lee, S. H., Jeong, H. J., Lee, C. S., & Kim, S. J. (2012). Curcumin protects against regional myocardial ischemia/reperfusion injury through activation of RISK/GSK-3beta and inhibition of p38 MAPK and JNK. Journal of Cardiovascular Pharmacology and Therapeutics, 17, 387–394.PubMedCrossRef
13.
Zurück zum Zitat Yang, Y., Hu, S. J., Li, L., & Chen, G. P. (2009). Cardioprotection by polysaccharide sulfate against ischemia/reperfusion injury in isolated rat hearts. Acta Pharmacologica Sinica, 30, 54–60.PubMedCentralPubMedCrossRef Yang, Y., Hu, S. J., Li, L., & Chen, G. P. (2009). Cardioprotection by polysaccharide sulfate against ischemia/reperfusion injury in isolated rat hearts. Acta Pharmacologica Sinica, 30, 54–60.PubMedCentralPubMedCrossRef
14.
Zurück zum Zitat Schwertz, H., Carter, J. M., Abdudureheman, M., Russ, M., Buerke, U., Schlitt, A., et al. (2007). Myocardial ischemia/reperfusion causes VDAC phosphorylation which is reduced by cardioprotection with a p38 MAP kinase inhibitor. Proteomics, 7, 4579–4588.PubMedCrossRef Schwertz, H., Carter, J. M., Abdudureheman, M., Russ, M., Buerke, U., Schlitt, A., et al. (2007). Myocardial ischemia/reperfusion causes VDAC phosphorylation which is reduced by cardioprotection with a p38 MAP kinase inhibitor. Proteomics, 7, 4579–4588.PubMedCrossRef
15.
Zurück zum Zitat Lin, M., Lu, S. S., Wang, A. X., Qi, X. Y., Zhao, D., Wang, Z. H., et al. (2011). Apigenin attenuates dopamine-induced apoptosis in melanocytes via oxidative stress-related p38, c-Jun NH2-terminal kinase and Akt signaling. Journal of Dermatological Science, 63, 10–16.PubMedCrossRef Lin, M., Lu, S. S., Wang, A. X., Qi, X. Y., Zhao, D., Wang, Z. H., et al. (2011). Apigenin attenuates dopamine-induced apoptosis in melanocytes via oxidative stress-related p38, c-Jun NH2-terminal kinase and Akt signaling. Journal of Dermatological Science, 63, 10–16.PubMedCrossRef
16.
Zurück zum Zitat Noh, H. J., Sung, E. G., Kim, J. Y., Lee, T. J., & Song, I. H. (2010). Suppression of phorbol-12-myristate-13-acetate-induced tumor cell invasion by apigenin via the inhibition of p38 mitogen-activated protein kinase-dependent matrix metalloproteinase-9 expression. Oncology Reports, 24, 277–283.PubMed Noh, H. J., Sung, E. G., Kim, J. Y., Lee, T. J., & Song, I. H. (2010). Suppression of phorbol-12-myristate-13-acetate-induced tumor cell invasion by apigenin via the inhibition of p38 mitogen-activated protein kinase-dependent matrix metalloproteinase-9 expression. Oncology Reports, 24, 277–283.PubMed
17.
Zurück zum Zitat Huang, C. H., Kuo, P. L., Hsu, Y. L., Chang, T. T., Tseng, H. I., Chu, Y. T., et al. (2010). The natural flavonoid apigenin suppresses Th1- and Th2-related chemokine production by human monocyte THP-1 cells through mitogen-activated protein kinase pathways. Journal of Medicinal Food, 13, 391–398.PubMedCrossRef Huang, C. H., Kuo, P. L., Hsu, Y. L., Chang, T. T., Tseng, H. I., Chu, Y. T., et al. (2010). The natural flavonoid apigenin suppresses Th1- and Th2-related chemokine production by human monocyte THP-1 cells through mitogen-activated protein kinase pathways. Journal of Medicinal Food, 13, 391–398.PubMedCrossRef
18.
Zurück zum Zitat Olfert, E. D., Cross, B. M., & McWilliam, A. A. (1993). Guide to the care and use of experimental animals. Vol. 1. No. 2. Ottawa: Canadian Council on Animal Care. Olfert, E. D., Cross, B. M., & McWilliam, A. A. (1993). Guide to the care and use of experimental animals. Vol. 1. No. 2. Ottawa: Canadian Council on Animal Care.
19.
Zurück zum Zitat Zheng, Z., Yang, M., Zhang, F., Yu, J., Wang, J., Ma, L., et al. (2011). Gender-related difference of sevoflurane postconditioning in isolated rat hearts: Focus on phosphatidylinositol-3-kinase/Akt signaling. Journal of Surgical Research, 170, e3–e9.PubMedCrossRef Zheng, Z., Yang, M., Zhang, F., Yu, J., Wang, J., Ma, L., et al. (2011). Gender-related difference of sevoflurane postconditioning in isolated rat hearts: Focus on phosphatidylinositol-3-kinase/Akt signaling. Journal of Surgical Research, 170, e3–e9.PubMedCrossRef
20.
Zurück zum Zitat Miao, Q., Wang, S., Miao, S., Wang, J., Xie, Y., & Yang, Q. (2011). Cardioprotective effect of polydatin against ischemia/reperfusion injury: Roles of protein kinase C and mito K(ATP) activation. Phytomedicine, 19, 8–12.PubMedCrossRef Miao, Q., Wang, S., Miao, S., Wang, J., Xie, Y., & Yang, Q. (2011). Cardioprotective effect of polydatin against ischemia/reperfusion injury: Roles of protein kinase C and mito K(ATP) activation. Phytomedicine, 19, 8–12.PubMedCrossRef
21.
Zurück zum Zitat Omura, T., Yoshiyama, M., Ishikura, F., Kobayashi, H., Takeuchi, K., Beppu, S., et al. (2001). Myocardial ischemia activates the JAK-STAT pathway through angiotensin II signaling in in vivo myocardium of rats. Journal of Molecular and Cellular Cardiology, 33, 307–316.PubMedCrossRef Omura, T., Yoshiyama, M., Ishikura, F., Kobayashi, H., Takeuchi, K., Beppu, S., et al. (2001). Myocardial ischemia activates the JAK-STAT pathway through angiotensin II signaling in in vivo myocardium of rats. Journal of Molecular and Cellular Cardiology, 33, 307–316.PubMedCrossRef
22.
Zurück zum Zitat Hu, J., Wang, Z., Guo, Y. Y., Zhang, X. N., Xu, Z. H., Liu, S. B., et al. (2009). A role of periaqueductal grey NR2B-containing NMDA receptor in mediating persistent inflammatory pain. Molecular Pain, 5, 71.PubMedCentralPubMedCrossRef Hu, J., Wang, Z., Guo, Y. Y., Zhang, X. N., Xu, Z. H., Liu, S. B., et al. (2009). A role of periaqueductal grey NR2B-containing NMDA receptor in mediating persistent inflammatory pain. Molecular Pain, 5, 71.PubMedCentralPubMedCrossRef
23.
Zurück zum Zitat Pagliaro, P., Mancardi, D., Rastaldo, R., Penna, C., Gattullo, D., Miranda, K. M., et al. (2003). Nitroxyl affords thiol-sensitive myocardial protective effects akin to early preconditioning. Free Radical Biology and Medicine, 34, 33–43.PubMedCrossRef Pagliaro, P., Mancardi, D., Rastaldo, R., Penna, C., Gattullo, D., Miranda, K. M., et al. (2003). Nitroxyl affords thiol-sensitive myocardial protective effects akin to early preconditioning. Free Radical Biology and Medicine, 34, 33–43.PubMedCrossRef
24.
Zurück zum Zitat Cory, S., & Adams, J. M. (2002). The Bcl2 family: Regulators of the cellular life-or-death switch. Nature Reviews Cancer, 2, 647–656.PubMedCrossRef Cory, S., & Adams, J. M. (2002). The Bcl2 family: Regulators of the cellular life-or-death switch. Nature Reviews Cancer, 2, 647–656.PubMedCrossRef
25.
Zurück zum Zitat Antonsson, B. (2004). Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways. Molecular and Cellular Biochemistry, 256–257, 141–155.PubMedCrossRef Antonsson, B. (2004). Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways. Molecular and Cellular Biochemistry, 256–257, 141–155.PubMedCrossRef
26.
Zurück zum Zitat Kuwana, T., Mackey, M. R., Perkins, G., Ellisman, M. H., Latterich, M., Schneiter, R., et al. (2002). Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane. Cell, 111, 331–342.PubMedCrossRef Kuwana, T., Mackey, M. R., Perkins, G., Ellisman, M. H., Latterich, M., Schneiter, R., et al. (2002). Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane. Cell, 111, 331–342.PubMedCrossRef
27.
Zurück zum Zitat Burlacu, A. (2003). Regulation of apoptosis by Bcl-2 family proteins. Journal of Cellular and Molecular Medicine, 7, 249–257.PubMedCrossRef Burlacu, A. (2003). Regulation of apoptosis by Bcl-2 family proteins. Journal of Cellular and Molecular Medicine, 7, 249–257.PubMedCrossRef
28.
Zurück zum Zitat Ling, Q., Xu, X., Wei, X., Wang, W., Zhou, B., Wang, B., et al. (2011). Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c. Journal of Experimental and Clinical Cancer Research, 30, 66.PubMedCentralPubMedCrossRef Ling, Q., Xu, X., Wei, X., Wang, W., Zhou, B., Wang, B., et al. (2011). Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c. Journal of Experimental and Clinical Cancer Research, 30, 66.PubMedCentralPubMedCrossRef
29.
Zurück zum Zitat Yue, T. L., Wang, C., Gu, J. L., Ma, X. L., Kumar, S., Lee, J. C., et al. (2000). Inhibition of extracellular signal-regulated kinase enhances ischemia/reoxygenation-induced apoptosis in cultured cardiac myocytes and exaggerates reperfusion injury in isolated perfused heart. Circulation Research, 86, 692–699.PubMedCrossRef Yue, T. L., Wang, C., Gu, J. L., Ma, X. L., Kumar, S., Lee, J. C., et al. (2000). Inhibition of extracellular signal-regulated kinase enhances ischemia/reoxygenation-induced apoptosis in cultured cardiac myocytes and exaggerates reperfusion injury in isolated perfused heart. Circulation Research, 86, 692–699.PubMedCrossRef
30.
Zurück zum Zitat Antonsson, B. (2001). Bax and other pro-apoptotic Bcl-2 family “killer-proteins” and their victim the mitochondrion. Cell and Tissue Research, 306, 347–361.PubMedCrossRef Antonsson, B. (2001). Bax and other pro-apoptotic Bcl-2 family “killer-proteins” and their victim the mitochondrion. Cell and Tissue Research, 306, 347–361.PubMedCrossRef
31.
Zurück zum Zitat Alnemri, E. S., Livingston, D. J., Nicholson, D. W., Salvesen, G., Thornberry, N. A., Wong, W. W., et al. (1996). Human ICE/CED-3 protease nomenclature. Cell, 87, 171.PubMedCrossRef Alnemri, E. S., Livingston, D. J., Nicholson, D. W., Salvesen, G., Thornberry, N. A., Wong, W. W., et al. (1996). Human ICE/CED-3 protease nomenclature. Cell, 87, 171.PubMedCrossRef
32.
Zurück zum Zitat Thomas, C. J., Ng, D. C., Patsikatheodorou, N., Limengka, Y., Lee, M. W., Darby, I. A., et al. (2011). Cardioprotection from ischaemia-reperfusion injury by a novel flavonol that reduces activation of p38 MAPK. European Journal of Pharmacology, 658, 160–167.PubMedCrossRef Thomas, C. J., Ng, D. C., Patsikatheodorou, N., Limengka, Y., Lee, M. W., Darby, I. A., et al. (2011). Cardioprotection from ischaemia-reperfusion injury by a novel flavonol that reduces activation of p38 MAPK. European Journal of Pharmacology, 658, 160–167.PubMedCrossRef
33.
Zurück zum Zitat Kaiser, R. A., Bueno, O. F., Lips, D. J., Doevendans, P. A., Jones, F., Kimball, T. F., et al. (2004). Targeted inhibition of p38 mitogen-activated protein kinase antagonizes cardiac injury and cell death following ischemia-reperfusion in vivo. Journal of Biological Chemistry, 279, 15524–15530.PubMedCrossRef Kaiser, R. A., Bueno, O. F., Lips, D. J., Doevendans, P. A., Jones, F., Kimball, T. F., et al. (2004). Targeted inhibition of p38 mitogen-activated protein kinase antagonizes cardiac injury and cell death following ischemia-reperfusion in vivo. Journal of Biological Chemistry, 279, 15524–15530.PubMedCrossRef
34.
Zurück zum Zitat Li, G., Barrett, E. J., Barrett, M. O., Cao, W., & Liu, Z. (2007). Tumor necrosis factor-alpha induces insulin resistance in endothelial cells via a p38 mitogen-activated protein kinase-dependent pathway. Endocrinology, 148, 3356–3363.PubMedCrossRef Li, G., Barrett, E. J., Barrett, M. O., Cao, W., & Liu, Z. (2007). Tumor necrosis factor-alpha induces insulin resistance in endothelial cells via a p38 mitogen-activated protein kinase-dependent pathway. Endocrinology, 148, 3356–3363.PubMedCrossRef
35.
Zurück zum Zitat Bassi, R., Heads, R., Marber, M. S., & Clark, J. E. (2008). Targeting p38-MAPK in the ischaemic heart: Kill or cure? Current Opinion in Pharmacology, 8, 141–146.PubMedCrossRef Bassi, R., Heads, R., Marber, M. S., & Clark, J. E. (2008). Targeting p38-MAPK in the ischaemic heart: Kill or cure? Current Opinion in Pharmacology, 8, 141–146.PubMedCrossRef
36.
Zurück zum Zitat Ma, L., Liu, H., Xie, Z., Yang, S., Xu, W., Hou, J., et al. (2014). Ginsenoside Rb3 protects cardiomyocytes against ischemia–reperfusion injury via the inhibition of JNK-mediated NF-κB pathway: A mouse cardiomyocyte model. PLoS ONE, 9, e103628.PubMedCentralPubMedCrossRef Ma, L., Liu, H., Xie, Z., Yang, S., Xu, W., Hou, J., et al. (2014). Ginsenoside Rb3 protects cardiomyocytes against ischemia–reperfusion injury via the inhibition of JNK-mediated NF-κB pathway: A mouse cardiomyocyte model. PLoS ONE, 9, e103628.PubMedCentralPubMedCrossRef
37.
Zurück zum Zitat Nicholas, R. L., Colleen, J. T., Lokugan, S. S., Yvonne, Y. Y., Suwan, Y., James, R. B., et al. (2013). Cardioprotective 3prime, 4prime-dihydroxyflavonol attenuation of JNK and p38MAPK signalling involves CaMKII inhibition. Biochemical Journal, 456, 149–161.CrossRef Nicholas, R. L., Colleen, J. T., Lokugan, S. S., Yvonne, Y. Y., Suwan, Y., James, R. B., et al. (2013). Cardioprotective 3prime, 4prime-dihydroxyflavonol attenuation of JNK and p38MAPK signalling involves CaMKII inhibition. Biochemical Journal, 456, 149–161.CrossRef
38.
Zurück zum Zitat Jones, W. K., Brown, M., Ren, X., He, S., & McGuinness, M. (2003). NF-κB as an integrator of diverse signaling pathways. Cardiovascular Toxicology, 3, 229–253.PubMedCrossRef Jones, W. K., Brown, M., Ren, X., He, S., & McGuinness, M. (2003). NF-κB as an integrator of diverse signaling pathways. Cardiovascular Toxicology, 3, 229–253.PubMedCrossRef
39.
Zurück zum Zitat Chen, H.-M., Hsu, J.-H., Liou, S.-F., Chen, T.-J., Chen, L.-Y., Chiu, C.-C., et al. (2014). Baicalein, an active component of Scutellaria baicalensis Georgi, prevents lysophosphatidylcholine-induced cardiac injury by reducing reactive oxygen species production, calcium overload and apoptosis via MAPK pathways. BMC complementary and alternative medicine, 14, 233.PubMedCentralPubMedCrossRef Chen, H.-M., Hsu, J.-H., Liou, S.-F., Chen, T.-J., Chen, L.-Y., Chiu, C.-C., et al. (2014). Baicalein, an active component of Scutellaria baicalensis Georgi, prevents lysophosphatidylcholine-induced cardiac injury by reducing reactive oxygen species production, calcium overload and apoptosis via MAPK pathways. BMC complementary and alternative medicine, 14, 233.PubMedCentralPubMedCrossRef
Metadaten
Titel
Protective Effect of Apigenin on Ischemia/Reperfusion Injury of the Isolated Rat Heart
verfasst von
Jing Hu
Zilin Li
Li-ting Xu
Ai-jun Sun
Xiao-yan Fu
Li Zhang
Lin-lin Jing
An-dong Lu
Yi-fei Dong
Zheng-ping Jia
Publikationsdatum
01.07.2015
Verlag
Springer US
Erschienen in
Cardiovascular Toxicology / Ausgabe 3/2015
Print ISSN: 1530-7905
Elektronische ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-014-9290-y

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