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Effect of Sulforaphane on Growth Inhibition in Human Brain Malignant Glioma GBM 8401 Cells by Means of Mitochondrial- and MEK/ERK-Mediated Apoptosis Pathway

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Abstract

In recent studies, sulforaphane (SFN) has been seen to demonstrate antioxidant and anti-tumor activities as well as potent chemopreventive action against cancer. The present study investigates the anti-proliferation (using MTT assay, SFN demonstrated cytotoxic activity against GBM 8401 cell with IC50 values at 35.52 μM) and induced apoptosis of SFN 24-h treatment in the cells of human brain malignant glioma GBM 8401 cells. We studied the MMP, caspase, MEK/ERK activation, and NF-κB transcription factor activity. Our results indicate that SFN inhibits cell proliferation as well as the activation of apoptosis in GBM 8401 cells. Both effects increased in proportion to the dosage of SFN, and apoptosis was induced via mitochondria- and caspase-dependent pathways. Daily s.c. injections of SFN for 3 weeks in severe combined immunodeficient mice (SCID) with GBM8401 s.c. tumors resulted in a decrease in mean tumor weight of 69–75 % compared with vehicle-treated controls. Our findings suggest that, in addition to the known effects on cancer prevention, SFN may provide antitumor activity in established malignant glioma.

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Acknowledgments

The authors gratefully acknowledge the financial support from Tainan Sin-Lau Hospital (99-01 and 100-01).

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Authors and Affiliations

  1. Department of Neurosurgery, Tainan Sin-Lau Hospital, Tainan, Taiwan

    Tzuu-Yuan Huang

  2. Graduate Institute of Medical Science, College of Health Sciences, Chang Jung Christian University, Tainan, Taiwan

    Weng-Cheng Chang & Yi-Chiang Hsu

  3. Innovative Research Center of Medicine, College of Health Sciences, Chang Jung Christian University, Tainan, Taiwan

    Miin-Yau Wang, Yun-Ru Yang & Yi-Chiang Hsu

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  1. Tzuu-Yuan Huang
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Correspondence to Tzuu-Yuan Huang or Yi-Chiang Hsu.

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Huang, TY., Chang, WC., Wang, MY. et al. Effect of Sulforaphane on Growth Inhibition in Human Brain Malignant Glioma GBM 8401 Cells by Means of Mitochondrial- and MEK/ERK-Mediated Apoptosis Pathway. Cell Biochem Biophys 63, 247–259 (2012). https://doi.org/10.1007/s12013-012-9360-3

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