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Effect of HSP70 and 90 in Modulation of JNK, ERK Expression in Preeclamptic Placental Endothelial Cell

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Abstract

Preeclampsia is a disease of worldwide significance with increasing maternal mortality rate of 20–80 %. Though apoptosis is a normal constituent during pregnancy, there seems to be an altered balance between proliferation and apoptosis of endothelial cell in preeclampsia leading to a placental dysregulation resulting in premature delivery. Molecular chaperones like HSP70 and 90 play a significant role in control of preeclamptic progression and protect the developing fetus. This is governed by alterations in expression of HSF1, HIF1α a nuclear transcription factor and signaling molecule like ERK, JNK1/2, and Bcl-2. Endothelial cell from normotensive and preeclamptic placenta were analyzed for variation in viability and expression of signaling molecules. A significant decrease in viability of endothelial cell (p < 0.05) was noted in preeclamptic samples when compared to normotensive samples. The results indicate that there was an increase in, HSP70 and 90 (p < 0.01), HSF1 (p < 0.01), HIF1α (p < 0.05), ERK (p < 0.05), JNK1/2 (p < 0.05), and Bcl-2 (p < 0.05). Though there is a significant change in the viability of endothelial cell, the live fetal delivery is not predominantly affected during preeclampsia. The interplay between these signaling molecules which alter the apoptotic pathway to sustain endothelial cell viability is discussed.

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Abbreviations

HSP:

Heat shock protein

HSF:

Heat shock factor

HIF:

Hypoxia inducible factor

ERK:

Extracellular signal regulating kinase

JNK:

c-jun N-terminal kinase

Bcl-2:

B cell CLL/lymphoma like protein-2

Elk:

ETS (E-twenty-six)-like kinase

TCF:

Ternary complex factor

MAPK:

Mitogen activate protein kinase

XO:

Xanthine oxidase

BMI:

Body mass index

EDTA:

Ethylene diamine tetra acetic acid

CD31:

Cluster of differentiation 31

PBS:

Phosphate-buffered saline

TEM:

Transmission electron microscopy

ELISA:

Enzyme linked immunosorbent assay

PVDF:

Polyvinylidene di-flouride

SDS:

Sodium dodecyl sulfate

BCIP–NBT:

Bromo chloro indolyl phenol–nitro blue tetrazolium

TBS:

Tris-buffered saline

DAB:

Diaminobenzedine

ANOVA:

Analysis of variance

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Acknowledgments

The project funded by National Tea Research Foundation, Tea Board of India is acknowledged. Project referral number—NTRF: 115/07. V. Uthra and S. Lavanya thank Indian council for Medical Research for providing financial assistance in the form of senior research fellowship.

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  1. P.G. Department of Biochemistry, Bharathi Women’s College, Affiliated to University of Madras, Chennai, Tamil Nadu, India

    Ekambaram Padmini, Venkatraman Uthra & Srinivasan Lavanya

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  1. Ekambaram Padmini
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Correspondence to Ekambaram Padmini.

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Padmini, E., Uthra, V. & Lavanya, S. Effect of HSP70 and 90 in Modulation of JNK, ERK Expression in Preeclamptic Placental Endothelial Cell. Cell Biochem Biophys 64, 187–195 (2012). https://doi.org/10.1007/s12013-012-9371-0

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