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Immunohistochemical Expression of RAGE and Its Ligand (S100A9) in Cervical Lesions

  • Translational Biomedical Research
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Abstract

Altered expressions of receptor for advanced glycation end-products (RAGE) and its ligand (S100A9) are observed in many cancers and play a key role in inflammation-associated cancer. In our previous study, by two-dimensional gel electrophoresis followed by mass spectrometry, the expression of S100A9 protein was found to increase in squamous cervical cancer compared with adjacent normal cervical tissues. Therefore, in the present study we observed the expressions of S100A9 and RAGE in 30 chronic cervicitis, 50 cervical intraepithelial neoplasia (CIN), and 40 squamous cervical cancer (SCC) using immunohistochemical analysis and analyzed the differential expression and possible role of S100A9 and RAGE in cancer development. Immunohistochemical findings were as follows: the expressions of S100A9 and RAGE were demonstrated in chronic cervicitis, CIN, and SCC. Moreover, their expressions were gradually increasing as the tumor progressed. In SCC, the staining scores of S100A9 and RAGE were significantly higher in well-differentiated tumors compared to moderately and poorly differentiated tumors. The expression of S100A9 in epithelial cells exhibited a positive correlation to RAGE expression in chronic cervicitis, CIN, and SCC. There were no significant difference of S100A9 immunoreactivity in stromal cells among chronic cervicitis, CIN, and SCC. Moreover, there was no correlation between S100A9 immunoreactivity in stromal cells of SCC and clinicopathological parameters. Finally, double immunohistochemistry illustrated that RAGE and S100A9 co-express in SCC. In conclusion, RAGE binds its ligand (S100A9), which plays an important role in the development of SCC. In addition, the expressions of S100A9 and RAGE in SCC tumor cells were closely associated with histological differentiation.

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Abbreviations

RAGE:

Receptor for advanced glycation end products

CIN:

Cervical intraepithelial neoplasia

SCC:

Squamous cervical cancer

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Acknowledgments

This study was supported by the Special Foundation for Outstanding Young Scientists of Zhejiang Province, China (Grant No. 2009QN023), and a grant of science and technology project in Wenzhou (No. Y20080153).

Conflict of interest

All the authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, People’s Republic of China

    Xuejie Zhu

  2. Department of Obstetrics and Gynecology, Second Affiliated Hospital of Wenzhou Medical College, No. 109 Xueyuan Xi Road, Wenzhou, 325027, Zhejiang, People’s Republic of China

    Lanying Jin, Shuangwei Zou, Qi Shen, Wenxiao Jiang, Wenjing Lin & Xueqiong Zhu

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  1. Xuejie Zhu
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  2. Lanying Jin
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Correspondence to Xueqiong Zhu.

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Zhu, X., Jin, L., Zou, S. et al. Immunohistochemical Expression of RAGE and Its Ligand (S100A9) in Cervical Lesions. Cell Biochem Biophys 66, 843–850 (2013). https://doi.org/10.1007/s12013-013-9515-x

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