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Erschienen in: Immunologic Research 3/2016

07.01.2016 | Original Article

Heterogeneous clinical spectrum of anti-SRP myositis and importance of the methods of detection of anti-SRP autoantibodies: a multicentric study

verfasst von: Cécile Picard, Thierry Vincent, Jean-Christophe Lega, Sophie Hue, Françoise Fortenfant, Daniela Lakomy, René-Louis Humbel, Joelle Goetz, Nicolas Molinari, Nathalie Bardin, Daniel Bertin, Catherine Johanet, Pascale Chretien, Sylvain Dubucquoi, Nathalie Streichenberger, Sophie Desplat-Jégo, Xavier Bossuyt, Jean Sibilia, Isabelle Abreu, Alain Chevailler, Nicole Fabien

Erschienen in: Immunologic Research | Ausgabe 3/2016

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Abstract

Anti-signal recognition particle (SRP) antibodies are important serological markers for the diagnosis and the prognosis of idiopathic inflammatory myopathy (IIM), especially to distinguish immune-mediated necrotizing myopathy (IMNM). This study was set up to investigate the phenotype associated with anti-SRP antibodies and to evaluate the methods for detecting these antibodies. Clinical and biological data were retrospectively obtained from 60 adult patients with anti-SRP antibodies detected by a dot immunoassay from 12 centers. Thirty-six (60 %) out of these 60 patients suffered from an IIM, and among them, 21 patients were diagnosed as IMNM. Among patients with a definite IIM, proximal weakness and myalgia were prominent symptoms at the time of diagnosis. Only few patients displayed severe extra-muscular symptoms such as cardiac involvement or severe myositis. Mean creatine kinase levels were high for all patients except for two of them. When testing by indirect immunofluorescence (IIF) on HEp2 cells, the fraction of patients displaying the typical anti-SRP fine speckled staining of the cytoplasm was higher in patients with IIM (30/36) (83 %) than in patients with non-IIM (3/24) (12.5 %) (p < 0.0001). Thirty (91 %) out of 33 patients with a positive immunodot and a characteristic IIF cytoplasmic staining suffered from a clinical definite myositis, whereas only 6 (22 %) out of 27 patients with a positive immunodot but a negative cytoplasmic pattern suffered from a myositis (p < 0.00001). This series highlights the strong heterogeneity of anti-SRP positivity that encompassed IMNM and non-IMNM and supports the necessity of considering both IIF and dot immunoassay to confirm the diagnosis of anti-SRP-associated myositis.
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Metadaten
Titel
Heterogeneous clinical spectrum of anti-SRP myositis and importance of the methods of detection of anti-SRP autoantibodies: a multicentric study
verfasst von
Cécile Picard
Thierry Vincent
Jean-Christophe Lega
Sophie Hue
Françoise Fortenfant
Daniela Lakomy
René-Louis Humbel
Joelle Goetz
Nicolas Molinari
Nathalie Bardin
Daniel Bertin
Catherine Johanet
Pascale Chretien
Sylvain Dubucquoi
Nathalie Streichenberger
Sophie Desplat-Jégo
Xavier Bossuyt
Jean Sibilia
Isabelle Abreu
Alain Chevailler
Nicole Fabien
Publikationsdatum
07.01.2016
Verlag
Springer US
Erschienen in
Immunologic Research / Ausgabe 3/2016
Print ISSN: 0257-277X
Elektronische ISSN: 1559-0755
DOI
https://doi.org/10.1007/s12026-015-8774-6

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