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PACAP Application Improves Functional Outcome of Chronic Retinal Ischemic Injury in Rats-Evidence From Electroretinographic Measurements

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Abstract

Retinoprotective effects of pituitary adenylate cyclase activating polypeptide (PACAP) are well-known and have been demonstrated in various pathological conditions, such as diabetic retinopathy, excitotoxic retinal injury, UV light-induced degeneration, and ischemic retinal lesion. The neuronal degeneration observed in the different retinal layers under the above pathological conditions can be successfully decreased by PACAP; however, whether this morphological improvement is also reflected in functional amelioration remains unknown. Therefore, our purpose was to investigate the protective effect of PACAP on the rat retina after bilateral common carotid artery occlusion (BCCAO) with electroretinography (ERG) to parallel the functional data with the previous morphological and neurochemical observations. Control eyes received saline treatment while PACAP was injected into the vitreous space of the other eye immediately after the induction of ischemia. Retinal damage and protective effects of PACAP were quantified by the changes in the wave forms and amplitudes. On postoperative days 2 and 14, several parameters were assessed with special attention to the changes of b wave. The results confirm that the previously described morphological protection induced by PACAP treatment is reflected in functional improvement in ischemic retinal lesions.

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Acknowledgments

This work was supported by PTE-MTA “Lendulet” Program, Arimura Foundation, OTKA K104984, K100144, PD 109644, TAMOP 4.2.2.A-11/1/KONV-2012-0024, TAMOP 4.2.4.A/2-11-1-2012-0001 “National Excellence Program,” Bolyai Scholarship.

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Authors and Affiliations

  1. Department of Anatomy, MTA-PTE “Lendulet” PACAP Research Team, University of Pecs, Szigeti u 12, Pecs, 7624, Hungary

    Bese Danyadi, Dora Reglodi, Agoston Mihalik, Tibor Danyadi, Andrea Tamas & Peter Kiss

  2. Department of Experimental Zoology and Neurobiology, University of Pecs, Pecs, Hungary

    Krisztina Szabadfi & Robert Gabriel

  3. Department of Anaesthesiology and Intensive Therapy, University of Pecs, Pecs, Hungary

    Istvan Batai

  4. Department of Zoology, The University of West Hungary, Savaria Campus, Szombathely, Hungary

    Zsolt Kovacs

  5. Department of Medical Chemistry, University of Szeged, Szeged, Hungary

    Gabor Toth

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  1. Bese Danyadi
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Correspondence to Dora Reglodi.

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Danyadi, B., Szabadfi, K., Reglodi, D. et al. PACAP Application Improves Functional Outcome of Chronic Retinal Ischemic Injury in Rats-Evidence From Electroretinographic Measurements. J Mol Neurosci 54, 293–299 (2014). https://doi.org/10.1007/s12031-014-0296-5

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  • DOI: https://doi.org/10.1007/s12031-014-0296-5

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