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Crossreactivity of an Antiserum Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae with the SNARE-Complex Protein Snap23 Correlates to Impaired Exocytosis in SH-SY5Y Cells

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Abstract

Early maternal infections with Neisseria gonorrhoeae (NG) correlate to an increased lifetime schizophrenia risk for the offspring, which might be due to an immune-mediated mechanism. Here, we investigated the interactions of polyclonal antisera to NG (α-NG) with a first trimester prenatal brain multiprotein array, revealing among others the SNARE-complex protein Snap23 as a target antigen for α-NG. This interaction was confirmed by Western blot analysis with a recombinant Snap23 protein, whereas the closely related Snap25 failed to interact with α-NG. Furthermore, a polyclonal antiserum to the closely related bacterium Neisseria meningitidis (α-NM) failed to interact with both proteins. Functionally, in SH-SY5Y cells, α-NG pretreatment interfered with both insulin-induced vesicle recycling, as revealed by uptake of the fluorescent endocytosis marker FM1-43, and insulin-dependent membrane translocation of the glucose transporter GluT4. Similar effects could be observed for an antiserum raised directly to Snap23, whereas a serum to Snap25 failed to do so. In conclusion, Snap23 seems to be a possible immune target for anti-gonococcal antibodies, the interactions of which seem at least in vitro to interfere with vesicle-associated exocytosis. Whether these changes contribute to the correlation between maternal gonococcal infections and psychosis in vivo remains still to be clarified.

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Acknowledgments

We would like to thank the University Medicine Göttingen (UMG) for persistent and reliable support of our work.

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Authors and Affiliations

  1. Institute for Neuroanatomy, University Medical Center Göttingen, Kreuzbergring 36, 37075, Göttingen, Federal Republic of Germany

    A. Almamy & B. Reuss

  2. University Children’s Hospital Mannheim, Pediatric Infectious Diseases, Heidelberg University, Heidelberg, Germany

    C. Schwerk & H. Schroten

  3. The Nippon Dental University, Tokyo, Japan

    H. Ishikawa

  4. Institute for Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany

    A. R. Asif

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  1. A. Almamy
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  2. C. Schwerk
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Almamy, A., Schwerk, C., Schroten, H. et al. Crossreactivity of an Antiserum Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae with the SNARE-Complex Protein Snap23 Correlates to Impaired Exocytosis in SH-SY5Y Cells. J Mol Neurosci 62, 163–180 (2017). https://doi.org/10.1007/s12031-017-0920-2

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