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Erschienen in: Medical Oncology 3/2010

01.09.2010 | Original Paper

MicroRNA-9 reduces cell invasion and E-cadherin secretion in SK-Hep-1 cell

verfasst von: Tan Hao-Xiang, Wang Qian, Chen Lian-Zhou, Huang Xiao-Hui, Chen Jin-Song, Fu Xin-Hui, Cao Liang-Qi, Chen Xi-Ling, Li Wen, Zhang Long-juan

Erschienen in: Medical Oncology | Ausgabe 3/2010

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Abstract

MicroRNAs (miRNAs) are an abundant class of short noncoding RNAs that can posttranscriptionally regulate gene expression in animals. They are also involved in cancer initiation and progression, and their expression profiles serve as phenotypic signatures of different cancers. The roles played by microRNAs specifically in “micromanagement of metastasis” has been addressed only recently. The molecular mechanisms of hepatocellular carcinoma (HCC) metastasis are still poorly understood. Recent evidence implies genetic determinants of cancer metastasis. Because gene expression signature significantly differs between primary metastasis-free HCC and primary HCC with intrahepatic metastases, miRNA expression in those primary HCC may change correspondingly. The 28 up-regulated miRNAs, part of the reported miRNA profiles of HCC, were compared in primary HCC with or without metastases. Only eight miRNAs were found to be significantly up-regulated in primary HCC with metastases while miR-9 had the highest hold change. miR-9 was highly expressed in SK-Hep-1 cell when compared with other hepatoma cell lines and downregulation of miR-9 reduced SK-Hep-1 cell invasion. E-cadherin, a tumor invasion suppressor in HCC, was found to be a putative gene target of miR-9. E-cadherin was up-regulated by miR-9 inhibitor. The findings suggest miR-9 could be involved in HCC metastasis.
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Metadaten
Titel
MicroRNA-9 reduces cell invasion and E-cadherin secretion in SK-Hep-1 cell
verfasst von
Tan Hao-Xiang
Wang Qian
Chen Lian-Zhou
Huang Xiao-Hui
Chen Jin-Song
Fu Xin-Hui
Cao Liang-Qi
Chen Xi-Ling
Li Wen
Zhang Long-juan
Publikationsdatum
01.09.2010
Verlag
Springer US
Erschienen in
Medical Oncology / Ausgabe 3/2010
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-009-9264-2

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