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Expression of miR-145 and Its Target Proteins Are Regulated by miR-29b in Differentiated Neurons

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A Correction to this article was published on 03 July 2019

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Abstract

MicroRNAs (miRNAs) are emerging as the most potential regulator of neuronal development. Recent studies from our lab and elsewhere have demonstrated a direct role of miRNAs in regulating neuronal differentiation and synaptogenesis. MicroRNA-145, a miRNA identified to regulate pluripotency of stem cells, downregulates the protein levels of reprogramming transcription factors (RTFs) like OCT4, SOX2, and KLF4 (cell, 137,647–658,2009). Studies have shown that miR-145 is multifunctional and crucial for fate determination of neurons. In our recently published study, we have identified a set of miRNAs including miR-145 and miR-29b families differentially expressed in SH-SY5Y cells exposed sequentially with retinoic acid + brain-derived neurotrophic factor (RA+BDNF) for differentiation into mature neurons (Mol Neurobiol (2016) doi:https://doi.org/10.1007/s12035-016-0042-9). In the present study, we have identified the role of miR-29b in upregulation of miR-145, which is upregulated after exposure of RA+BDNF in a P53-dependent manner. In differentiating SH-SY5Y cells, expression of miR-29b downregulates expression of P85α, a P53 inhibitor, which results in upregulation of miR-145 and downregulation of RTF proteins. Ectopic expression of miR-145 and miR-29b in amounts equivalent to their endogenous expression has induced G1 phase cell cycle arrest. In conclusion, our studies have identified miR-29b as an upstream regulator of miR-145 and targets its RTF genes during differentiation of SH-SY5Y cells.

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Change history

  • 03 July 2019

    The original version of this article unfortunately contained an error. In Figure 8, the image under section a) NTC, and b) NTC���+���PFT�� are copied by mistake.

  • 03 July 2019

    The original version of this article unfortunately contained an error. In Figure 8, the image under section a) NTC, and b) NTC���+���PFT�� are copied by mistake.

Abbreviations

BDNF:

brain-derived neurotrophic factor

DTT:

Dithiothreitol

HPRT:

hypoxanthine-guanine phosphoribosyltransferase

IDV:

integrated density value

miRNA:

microRNA

mRNA:

messenger RNA

PCR:

polymerase chain reaction

RA:

retinoic acid

RTF:

reprogramming transcription factor

RQ:

relative quantification

RT:

reverse transcription

IR:

infrared

PBS:

phosphate buffer saline

PI:

propidium iodide

PLL:

poly-l-lysine

PVDF:

polyvinylidene fluoride

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Acknowledgments

Mr. Abhishek Jauhari is grateful to UGC, New Delhi, and Ms. Tanisha Singh is grateful to DST, New Delhi, for providing research fellowships. The technical assistance of Mr. B S Pandey and Mr. Puneet Khare is also gratefully acknowledged. The CSIR-IITR communication reference number is 3493.

Funding

Funding for the work carried out in the present study had been provided by the CSIR network project (miND).

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Authors and Affiliations

  1. Developmental Toxicology Laboratory, Systems Toxicology and Health Risk Assessment Group, CSIR- Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, -226001, India

    Abhishek Jauhari, Tanisha Singh & Sanjay Yadav

  2. Academy of Scientific and Innovative Research (AcSIR), CSIR- IITR Campus, New Delhi, India

    Abhishek Jauhari

  3. Department of Biochemistry, School of Dental Sciences, Babu Banarasi Das University, Lucknow, Uttar Pradesh, India

    Tanisha Singh

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  1. Abhishek Jauhari
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Correspondence to Sanjay Yadav.

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Jauhari, A., Singh, T. & Yadav, S. Expression of miR-145 and Its Target Proteins Are Regulated by miR-29b in Differentiated Neurons. Mol Neurobiol 55, 8978–8990 (2018). https://doi.org/10.1007/s12035-018-1009-9

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