Abstract
Human metapneumovirus is a recently recognized pathogen of acute respiratory tract infection (ARI) in children as well as elderly and immunocompromised adults. The virus belongs to the family Paramyxoviridae, sub family Pneumovirinae and genus Metapneumovirus. Through genetic analysis it has been characterized into two groups A and B which are further divided into four sub-lineages. The virus is difficult to grow in tissue culture and hence reverse transcriptase-polymerase chain reaction (RT-PCR) for N and L gene is the method of choice for diagnosis. The virus has been seen in all countries with seasonal distribution in winter months for temperate and spring/summer for tropical countries. F gene is the most conserved among different lineages and efforts are underway to design recombination vaccine using F gene.
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Abbreviations
- ALRI:
-
acute lower respiratory tract infection
- APV:
-
avian pneumoviruses
- ARI:
-
acute respiratory tract infection
- CPE:
-
cytopathic effect
- hMPV:
-
human metapneumovirus
- RSV:
-
respiratory syncytial virus
- RT-PCR:
-
reverse tracriptase-polymerase chain reaction
- SARS:
-
severe acute respiratory syndrome
- SH:
-
small hydrophobic
References
Agapov E, Sumino K C, Gaudreault-Keener M, Storch G A and Holtzman M J 2006 Genetic variability of human metapneumovirus infection: evidence of a shift in viral genotype without a change in illness; J. Infect. Dis. 193 396–403
Ahmad O B, Lopez A D and Inoue M 2000 The decline in child mortality: a reappraisal; Bull. World Health Org. 78 1175–1191
Al-Sonboli N, Hart C A, Al-Aeryani A, Banajeh S M, Al-Aghbari N, Dove W and Cuevas L E 2005 Respiratory syncytial virus and human Metapneumovirus in children with acute respiratory infections in Yemen; Pediatr. Infect. Dis. J. 24 734–736
Alvarez R, Harrod K S, Shieh W J, Zaki S and Tripp R A 2004 Human metapneumovirus persists in BALB/c mice despite the presence of neutralizing antibodies; J. Virol. 78 14003–14011
Alvarez R and Tripp R A 2005 The immune response to human metapneumovirus is associated with aberrant immunity and impaired virus clearance in BALB/c mice; J. Virol. 79 5971–5978
Banerjee S, Bharaj P, Sullender W, Kabra S K and Broor S 2007 Human Metapneumovirus infections among children with acute respiratory infections seen in a large referral hospital in India; J. Clin. Virol. 38 70–72
Bastein N, Normand S, Taylor T, Ward D, Peret T C, Boivin G, Anderson L J and Li Y 2003 Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains; Virus Res. 93 51–62
Biacchesi S, Skiadopoulos M H, Boivin G, Hanson C T, Murphy B R and Collins P L, Buchholz U J 2003 Genetic diversity between human metapneumovirus subgroups; Virology 315 1–9
Boivin G, Abed Y, Pelletier G, Ruel L, Moisan D, Cote S, Peret T C, Erdman D D and Anderson L J 2002 Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups; J. Infect. Dis. 186 1330–1334
Boivin G, De Serres G, Cote S, Gilca R, Abed Y, Rochette L, Bergeron L G and Dery P 2003 Human metapneumovirus infections in hospitalized children; Emerg. Infect. Dis. 9 634–640
Boivin G, Mackay I, Sloots T P, Madhi S, Freymuth F, Wolf D, Shemer-Avni Y, Ludewick H, et al 2004 Global genetic diversity of human metapneumovirus fusion gene; Emerg. Infect.Dis. 10 1154–1157
Chan E and Li Y 2003 Human metapneumovirus infection in the Canadian population; J. Clin. Microbiol. 41 4642–4646
Collins P L and Mottet G 1992 Oligomerization and post-translational processing of glycoprotein G of human respiratory syncytial virus: altered O-glycosylation in the presence of brefeldin -A; J. Gen. Virol 73 849–863
Collins P L and Mottet G 1993 Membrane orientation and oligomerization of the small hydrophobic protein of human respiratory Syncytial virus; J. Gen. Virol. 74 1445–1450
Collins P L, Chanock R M and Murphy B R 2001 Respiratory Syncytial virus; in Fields virology 4th edition (eds) D M Knipe, P M Howley, D E Griffin, R A Lamb, M A Martin, B Roizman and S E Straus (Philadelphia, Lippincott-Raven Publishers) vol. 1, pp 1443–1485
Cote S, Abed Y and Boivin G 2003 Comparative evaluation of real-time PCR assays for detection of the human Metapneumovirus; J. Clin. Microbiol. 41 3631–3635
Crowe E J 2004 Human Metapneumovirus as a Major Cause of Human Respiratory Tract Disease; Pediatr. Infect. Dis. J. 23 S215–S221
Cuevas L E, Nasser A M, Dove W, Gurgel R Q, Greensill J and Hart C A 2003 Human metapneumovirus and respiratory syncytial virus, Brazil; Emerg. Infect. Dis. 9 1626-1628
Dare R, Sanghavi S, Bullotta A, Keightley M C, George K S, Wadowsky R M, Paterson D L, McCurry K R, et al 2007 Diagnosis of human metapneumovirus infection in immunosuppressed lung transplant recipients and children evaluated for pertussis; J. Clin. Microbiol. 45 548–552
Denny F W and Loda F A 1986 Acute respiratory infections are the leading cause of death in children in developing countries; Am. J. Trop. Med. Hyg. 35 1–2
Dollner H, Risnes K, Radtke A and Nordbo S A 2004 Outbreak of Human metapneumovirus infection in Norwegian children; Pediatr. Infect. Dis. J. 23 436–440
Easton A J and Chambers P 1997 Nucleotide sequence of the genes encoding the matrix and small hydrophobic proteins of pneumonia virus of mice; Virus Res. 48 27–33
Easton A J, Domachowske J B and Rosenberg H F 2004 Animal pneumovirus: molecular genetics and pathogenesis; Clin. Microbiol. Rev. 17 390–412
Ebihara T, Endo R, Kikuta H, Ishiguro N, Ishiko H, Hara M, Takahashi Y and Kobayashi K 2004 Human metapneumovirus infection in Japanese children; J. Clin. Microbiol. 42 126–132
Ebihara T, Endo R, Ma X, Ishiguro N and Kikuta H 2005 Detection of human metapneumovirus antigens in nasopharyngeal secretions by an immunofluorescent-antibody test; J. Clin. Microbiol. 43 1138–1141
Esper F, Boucher D, Weibel C, Martinello R A and Kahn J S 2003 Human metapneumovirus infection in the United States: clinical manifestations associated with a newly emerging respiratory infection in children; Pediatrics 111 1407–1410
Esper F, Martinello R A, Boucher D, Weibel C, Ferguson D, Landry M L and Kahn J S 2004 A 1-year experience with human metapneumovirus in children aged −5 years; J. Infect. Dis. 189 1388–1396
Falsey A R, Erdman D D, Anderson L J and Walsh E E 2003 Human metapneumovirus infections in young and elderly adults; J. Infect. Dis. 187 785–790
Feldman S A, Audet A and Beeler J A 2000 The fusion glycoprotein of human respiratory syncytial virus facilitates virus attachment and infectivity via an interaction with cellular heparan sulfate; J. Virol. 74 6442–6447
Fouchier R A, Kuiken T, Schutten M, van Amerongen G, van Doornum G J, van den Hoogen B G, Peiris M, Lim W, Stöhr K and Osterhaus A D 2003 Aetiology: Koch’s postulates fulfilled for SARS virus; Nature (London) 15 240
Freymuth F A, Vabret L, Legrand N, Eterradossi F, Lafay-Delaire J Brouard and Guillois B 2003 Presence of the new human Metapneumovirus in French children with bronchiolitis; Pediatr. Infect. Dis. J. 22 92–94
Greensill J, McNamara P S, Dove W, Flanagan B, Smyth R L and Hart C A 2003 Human metapneumovirus in severe respiratory Syncytial virus bronchiolitis; Emerg. Infect. Dis. 9 372–375
Grondahl B, Puppe W, Hoppe A, Kuhne I, Weigl J A and Schmitt H J 1999 Rapid identification of nine micro-organisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study; J. Clin. Microbiol. 37 1–7
Hamelin M E, Abed Y and Boivin G 2004 Human metapneumovirus: a new player among respiratory viruses; Clin. Infect. Dis. 38 983–990
Hamelin M E and Boivin G 2005 Development and validation of an Enzyme-linked immunosorbent assay for human Metapneumovirus serology based on a recombinant viral protein; Clin. Diagn. Lab. Immunol. 12 249–253
Hamelin M E, Cote S, Laforge, Lampron N, Bourbeau J, Weiss K, Gilca R, DeSerres G and Boivin G 2005 Human metapneumovirus infection in adults with community-acquired pneumonia and exacerbation of chronic obstructive pulmonary disease; Clin. Infect. Dis. 41 498–502
Herfst S, De Graaf M, Schickli J H, et al 2004 Recovery of human metapneumovirus genetic lineages A and B from cloned cDNA; J. Virol. 78 8264–8270
Hijazi Z, Pacsa A, Eisa S, el Shazli A and abd el-Salam R A 1996 Laboratory diagnosis of acute lower respiratory tract viral infections in children; J. Trop. Pediatr. 42 276–280
Huck B, Scharf G, Neumann-Heifelin D, Puppe W, Weigl J and Falcone V 2006 Novel human metapneumovirus sublineage; Emerg. Infect. Dis. 12 147–150
Human Metapneumovirus 2006 Red Book 460–461
Jartti T, van den Hoogen B G, Garofalo R P, Osterhaus A D and Ruuskanen O 2002 Metapneumovirus and acute wheezing in children; Lancet 360 1393–1394
Kim Y K and Lee H J 2005 Human metapneumovirus-associated lower respiratory tract infections in korean infants and young children; Pediatr. Infect. Dis. J. 24 1111–1112
Kuiken T, van den Hoogen B G, van Riel D A, Laman J D, van Amerongen G, Sprong L, Fouchier R A and Osterhaus A D 2004 Experimental human metapneumovirus infection of cynomolgus macaques (Macaca fascicularis) results in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract; Am. J. Pathol. 164 1893–1900
Laham F R, Israele V, Casellas J M, Garcia A M, Lac Prugent C M, Hoffman S J, Hauer D, Thumar B, et al 2004 Differential production of inflammatory cytokines in primary infection with human metapneumovirus and with other common respiratory viruses of infancy; J. Infect. Dis. 189 2047–2056
Lamb R A, Collins P L, Kolakofsky D, Melero J A, Nagai Y, Oldstone M B A, et al 2000 Family paramyxoviridae; in Virus taxonomy (eds) M H V Van Regenmortel, C M Fauquet, D H L Bishop, E B Carstens, M K Estes, J Maniloff, et al (San Diego; Academic Press) pp 549–561
Landry M L, Ferguson D, Cohen S, Peret T C and Erdman D D 2005 Detection of human metapneumovirus in clinical samples by immunofluorescence staining of shell vial centrifugation cultures prepared from three different cell lines; J. Clin. Microbiol. 43 1950–1952
Leung J, Esper F, Weibel C and Kahn J S 2005 Seroepidemiology of human metapneumovirus (hMPV) on the basis of a novel enzyme-linked immunosorbent assay utilizing hMPV fusion protein expressed in recombinant vesicular stomatitis virus; J. Clin. Microbiol. 43 1213–1219
Levine S, Klaiber-Franco R and Paradiso P R 1987 Demonstration that glycoprotein G is the attachment protein of respiratory syncytial virus; J. Gen. Virol. 68 2521–2524
Liolios L, Jenney A, Spelman D, Kotsimbos T, Catton M and Wesselingh S 2001 Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens; J. Clin. Microbiol. 39 2779–2783
Liu L, Bastien N and Li Y 2007 Intracellular Processing, Glycosylation, and Cell Surface Expression of Human Metapneumovirus Attachment Glycoprotein; J. Virol. 81 13435–13443
Ludewick H P, Abed Y, van Niekerk N, Boivin G, Klugman K P and Madhi S A 2005 Human metapneumovirus genetic variability, South Africa; Emerg. Infect. Dis. 1 1074–1078
Mackay I M, Jacob K C, Woolhouse D, Waller K, Syrmis M W, Whiley D M, Siebert D J, Nissen M and Sloots T P 2003 Molecular assays for detection of human Metapneumovirus; J. Clin. Microbiol. 41 100–105
Mackay I, Bialasiewicz M S, Waliuzzaman Z, Chidlow G R, Fegredo D C, Laingam S, Adamson P, Harnett G B, Rawlinson W, Nissen M D and Sloots T P 2004 Use of the P gene to genotype human metapneumovirus identifies 4 viral subtypes; J. Infect. Dis. 190 1913–1918
MacPhail M, Schickli J H, Tang R S, Kaur J, Robinson C, Fouchier R A, Osterhaus A D, Spaete R R and Haller A A 2004 Identification of small-animal and primate models for evaluation of vaccine candidates for human metapneumovirus (hMPV) and implications for hMPV vaccine design; J. Gen. Virol. 85 1655–1663
Madhi S A, Ludewick H, Abed Y, Klugman K P and Boivin G 2003 Human metapneumovirus-associated lower respiratory tract infections among hospitalized human immunodeficiency virus type 1 (HIV-1)-infected and HIV-1-uninfected African infants; Clin. Infect. Dis. 37 1705–1710
Maertzdorf J, Wang C K, Brown J B, Quinto J D, Chu M, de Graaf M, van den Hoogen B G, Spaete R, Osterhaus A D and Fouchier R A 2004 Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages; J. Clin. Microbiol. 42 981–986
Maggi F, Pifferi M, Vatteroni M, Fornai C, Tempestini E, Anzilotti S, Lanini L, Andreoli E, Ragazzo V, Pistello M, Specter S and Bendinelli M 2003 Human metapneumovirus associated with respiratory tract Infections in a 3 year study of nasal swabs from infants in Italy; J. Clin. Microbiol. 41 2987–2991
McIntosh K and McAdam A J 2004 Human Metapneumovirus — an important new respiratory virus; N. Engl. J. Med. 350 431–433
Nissen, M D, Siebert D J, Mackay I M, Sloots T P and Withers S J 2002 Evidence of human metapneumovirus in Australian children; Med. J. Aust. 176 188
Nokso-Koivisto J, Pitkaranta A, Blomqvist S, Jokinen J, Kleemola M, Takala A, Kilpi T and Hovi T 2002 Viral etiology of frequently recurring respiratory tract infections in children; Clin. Infect. Dis. 35 540–546
Peiris J S, Tang W H, Chan K H, Khong P L, Guan Y, Lau Y L and Chiu S S 2003 Children with respiratory disease associated with metapneumovirus in Hong Kong; Emerg. Infect. Dis. 9 628–633
Pelletier G, Dery P, Abed Y and Boivin G 2002 Respiratory tract reinfections by the new human metapneumovirus in an immunocompromised child; Emerg. Infect. Dis. 8 976–978
Percivalle E, Sarasini A, Visai L, Revello L G and Gerna G 2005 Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies; J. Clin. Microbiol. 43 3443–3446
Peret T C, Boivin G, Li Y, Couillard M, Humphrey C, Osterhaus A D, Erdman D D and Anderson L J 2002 Characterization of human metapneumoviruses isolated from patients in North America; J. Infect. Dis. 185 1660–1663
Peret T C, Abed Y, Anderson L J, Erdman D D and Boivin G 2004 Sequence polymorphism of the predicted human metapneumovirus G glycoprotein; J. Gen. Virol. 85 679–686
Pham Q N, Biacchesi S, Skiadopoulos M H, Murphy B R, Collins P L and Buchholz U J 2005 Chimeric recombinant human metapneumoviruses with the nucleoprotein or phosphoprotein open reading frame replaced by that of avian metapneumovirus exhibit improved growth in vitro and attenuation in vivo; J. Virol. 79 15114–15122
Poutanen S M, Low D E, Henry B, Finkelstein S, Rose D, Green K, Tellier R, Draker R, et al 2003 Identification of severe acute respiratory syndrome in Canada; N. Engl. J. Med. 348 1995–2005
Prins J M and Wolthers K C 2004 Human metapneumovirus: a new pathogen in children and adults; Netherlands J. Med. 62 177–179
Rao B L, Gandhe S S, Pawar S D, Shah S C, Kinikar A A and Arankalle V A 2004 First Detection of Human Metapneumovirus in Children with Acute Respiratory Infection in India: a Preliminary Report; J. Clin Microbiol. 42 5961–5962
Rawlinson W D, Waliuzzaman Z, Carter I W, Belessis Y C, Gilbert K M and Morton J R 2003 Asthma exacerbations in children associated with rhinovirus but not human metapneumovirus infection; J. Infect. Dis. 187 1314–1318
Reddaiah V P and Kapoor S K 1988 Acute respiratory infections in rural Underfives; Indian J. Pediatr. 55 424–426
Reina J, Ferres F, Alcoceba E, Menaa A, de Gopegui E R and Figuerola J 2007 Comparison of different cell lines and incubation times in the isolation by the shell vial culture of human metapneumovirus from pediatric respiratory samples; J. Clin.Virol. 40 46–49
Samransamruajkit R, Thanasugarn W, Prapphal W, Theamboonlers W and Poovorawan Y 2005 Human metapneumovirus in infants and young children in Thailand with lower respiratory tract infections; molecular characteristics and clinical presentations; J. Infect. 52 254–263
Schildgen O and Simon A 2005 Induction of acute otitis media by human Metapneumovirus; Pediatr. Infect. Dis. J. 24 1126
Semple M G, Cowell A, Dove W, Greensill J, McNamara P S, Halfhide C, Shears P, Smyth R L and Hart C A 2005 Dual infection of infants by human metapneumovirus and human respiratory Syncytial virus is strongly associated with severe bronchiolitis; J. Infect. Dis. 191 382–386
Shapiro E 1998 Epidemiology of acute respiratory infections; Semin. Pediatr. Infect. Dis. 9 31–36
Skiadopoulos M H, Biacchesi s, Buchholz U J, Riggs J M, Surman S R, Amaro-Carambot E, McAuliffe J M, Elkins W R, et al 2004 The two major human Metapneumovirus genetic lineages are highly related antigenically and the fusion (F) protein is a major contributor to this antigenic relatedness; J. Virol. 78 6927–6937
Stockton J, Stephenson I, Fleming D and Zambon M 2002 Human metapneumovirus as a cause of community-acquired respiratory illness; Emerg. Infect. Dis. 8 897–901
Tang R S, Mahmood K, Macphail M, Guzzetta J M, Haller J M, Liu H, Kaur J, Lawlor H A, et al 2005 A host-range restricted Parainfluenza virus type 3 (PIV3) expressing the human metapneumovirus (hMPV) fusion protein elicits protective immunity in African green monkeys; Vaccine 23 1657–1667
van den Hoogen B G, de Jong J C, Groen J, Kuiken T, de Groot, R, Fouchier R A and Osterhaus A D 2001 A newly discovered human pneumovirus isolated from young children with respiratory tract disease; Nat. Med. 7 719–724
van den Hoogen B G, Bestebroer T M, Osterhaus A D and Fouchier R A 2002 Analysis of the genomic sequence of a human Metapneumovirus; Virology 295 119–132
van den Hoogen B G, van Doornum G J, Fockens J C, Cornelissen J J, Beyer W E, de Groot R, Osterhaus A D and Fouchier R A 2003 Prevalence and clinical symptoms of human metapneumovirus infection in hospitalized patients; J. Infect. Dis. 188 1571–1577
van den Hoogen B G, Herfst S, Sprong L, Cane P A, Forleo-Neto E, de Swart R L, Osterhaus A D and Fouchier R A 2004 Antigenic and genetic variability of human metapneumoviruses; Emerg. Infect. Dis. 10 658–666
Vargas S O, Kozakewich H P, Perez-Atayde A R and McAdam A J 2004 Pathology of human metapneumovirus infection: insights into the pathogenesis of a newly identified respiratory virus; Pediatr. Dev. Pathol. 7 478–486
Viazov S, Ratjen F, Scheidhauer R, Fiedler M and Roggendorf M 2003 High prevalence of human metapneumovirus infection in young children and genetic heterogeneity of the viral isolates; J. Clin. Microbiol. 41 3043–3045
Vicente D, Cilla G, Montes M and Pérez-Trallero E 2003 Human metapneumovirus and community-acquired respiratory illness in children; Emerg. Infect. Dis. 9 602–603
Vicente D, Montes M, Cilla G, Perez-Yarza E G and Perez-Trallero E 2006 Differences in clinical severity between genotype A and genotype B human metapneumovirus infection in children; Clin. Infect. Dis. 15 e111–e113
Victora C G, Kirkwood B R, Ashworth A, Black R E, Rogers S, Sazawal S, Campbell H and Gove S 1999 Potential interventions for the prevention of childhood pneumonia in developing countries: improving nutrition; Am. J. Clin. Nutr. 70 309–320
Weigl J A, Puppe W, Grondahl B and Schmitt H J 2000 Epidemiological investigation of nine respiratory pathogens in hospitalized children in Germany using multiplex reverse-transcriptase polymerase chain reaction; Eur. J. Clin. Microbiol. Infect. Dis. 19 336–343
Williams J V, Harris P A, Tollefson S J, Halburnt-Rush L L, Pingsterhaus J M, Edwards K M, Wright P F and Crowe J E Jr 2004 Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children; N. Engl. J. Med. 350 443–450
Williams B G, Gouws E, Boschi-Pinto C, Bryce J and Dye C 2002 Estimates of world wide distribution of child deaths from acute respiratory infections; Lancet Infect. Dis. 2 25–32
Wyde P R, Chetty S N, Jewell A M, Boivin G and Piedra P A 2003 Comparison of the inhibition of human metapneumovirus and respiratory syncytial virus by ribavirin and immune serum globulin in vitro; Antiviral Res. 60 51–59
Wyde P R, Moylett E H, Chetty E H, Jewell A M, Bowlin T L and Piedra P A 2004 Comparison of the inhibition of human metapneumovirus and respiratory syncytial virus by NMSO3 in tissue culture assays; Antiviral Res. 63 51–59
Xepapadaki P, Psarras S, Bossios A, Tsolia M, Gourgiotis D, Liapi-Adamidou G, Constantopoulos AG, Kafetzis D and Papadopoulos N G 2004 Human Metapneumovirus as a causative agent of acute bronchiolitis in infants; J. Clin. Virol. 30 267–270
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Broor, S., Bharaj, P. & Chahar, H.S. Human metapneumovirus: a new respiratory pathogen. J Biosci 33, 483–493 (2008). https://doi.org/10.1007/s12038-008-0067-y
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DOI: https://doi.org/10.1007/s12038-008-0067-y