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01.09.2009 | Original Paper

Hematopoietic- and Neurologic-Expressed Sequence 1 Expression in the Murine GL261 and High-Grade Human Gliomas

verfasst von: Katharine M. Laughlin, Defang Luo, Che Liu, Gerry Shaw, Kenneth H. Warrington Jr., Jingxin Qiu, Anthony T. Yachnis, Jeffrey K. Harrison

Erschienen in: Pathology & Oncology Research | Ausgabe 3/2009

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Abstract

The hematopoietic- and neurologic-expressed sequence 1 (Hn1) gene encodes a highly conserved protein that is expressed in developing and regenerating tissues. In this study, Hn1 expression was evaluated in human and murine malignant gliomas. Hn1 mRNA and protein were detected in the murine GL261 glioma cell line and in GL261 brain tumors in vivo. HN1 is also expressed in human U118MG and U87MG cell lines. Evaluation of human brain tumors using an anti-Hn1 polyclonal antibody detected strong immunoreactivity in high-grade (WHO III and IV) malignant gliomas. The rate of GL261 cell proliferation in vitro was unaltered by Hn1 depletion using an anti-Hn1 siRNA. However, tumors established from Hn1-depleted GL261 cells formed significantly smaller volumes than those established from control-treated cells. These data suggest a role for Hn1 in the biology of malignant brain tumors.

Zujovic V, Luo D, Baker HV, Lopez MC, Miller KR, Streit WJ, Harrison JK (2005) The facial motor nucleus transcriptional program in response to peripheral nerve injury identifies Hn1 as a regeneration-associated gene. J Neurosci Res 82:581–591CrossRefPubMed

Goto T, Hisatomi O, Kotoura M, Tokunaga F (2006) Induced expression of hematopoietic- and neurologic-expressed sequence 1 in retinal pigment epithelial cells during newt retina regeneration. Exp Eye Res 83:972–980CrossRefPubMed

Lu KH, Patterson AP, Wang L, Marquez RT, Atkinson EN, Baggerly KA, Ramoth LR, Rosen DG, Liu J, Hellstrom I, Smith D, Hartmann L, Fishman D, Berchuck A, Schmandt R, Whitaker R, Gershenson DM, Mills GB, Bast RC, Jr. (2004) Selection of potential markers for epithelial ovarian cancer with gene expression arrays and recursive descent partition analysis. Clin Cancer Res 10:3291–3300CrossRefPubMed

Tang W, Lai YH, Han XD, Wong PM, Peters LL, Chui DH (1997) Murine Hn1 on chromosome 11 is expressed in hemopoietic and brain tissues. Mamm Genome 8:695–696CrossRefPubMed

Zhou G, Wang J, Zhang Y, Zhong C, Ni J, Wang L, Guo J, Zhang K, Yu L, Zhao S (2004) Cloning, expression and subcellular localization of HN1 and HN1L genes, as well as characterization of their orthologs, defining an evolutionarily conserved gene family. Gene 331:115–123CrossRefPubMed

Zimmerman HM, Arnold H (1941) Experimental brain tumors. I. Tumors produced with methylcholanthrene. Cancer Res 919–924

Ausman JI, Shapiro WR, Rall DP (1970) Studies on the chemotherapy of experimental brain tumors: development of an experimental model. Cancer Res 30:2394–2400PubMed

Szatmari T, Lumniczky K, Desaknai S, Trajcevski S, Hidvegi EJ, Hamada H, Safrany G (2006) Detailed characterization of the mouse glioma 261 tumor model for experimental glioblastoma therapy. Cancer Sci 97:546–553CrossRefPubMed

San-Galli F, Vrignaud P, Robert J, Coindre JM, Cohadon F (1989) Assessment of the experimental model of transplanted C6 glioblastoma in Wistar rats. J Neurooncol 7:299–304CrossRefPubMed

10.

Weiner NE, Pyles RB, Chalk CL, Balko MG, Miller MA, Dyer CA, Warnick RE, Parysek LM (1999) A syngeneic mouse glioma model for study of glioblastoma therapy. J Neuropathol Exp Neurol 58:54–60CrossRefPubMed

11.

Ishii N, Tada M, Hamou MF, Janzer RC, Meagher-Villemure K, Wiestler OD, Tribolet N, Van Meir EG (1999) Cells with TP53 mutations in low grade astrocytic tumors evolve clonally to malignancy and are an unfavorable prognostic factor. Oncogene 18:5870–5878CrossRefPubMed

12.

Bos JL (1989) ras oncogenes in human cancer: a review. Cancer Res 49:4682–4689PubMed

13.

Sidransky D, Mikkelsen T, Schwechheimer K, Rosenblum ML, Cavanee W, Vogelstein B (1992) Clonal expansion of p53 mutant cells is associated with brain tumour progression. Nature 355:846–847CrossRefPubMed

14.

Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995CrossRefPubMed

15.

Harris J, Ayyub C, Shaw G (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47–62CrossRefPubMed

16.

McCarty DM, Monahan PE, Samulski RJ (2001) Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis. Gene Ther 8:1248–1254CrossRefPubMed

17.

Zolotukhin S, Potter M, Zolotukhin I, Sakai Y, Loiler S, Fraites TJ, Jr., Chiodo VA, Phillipsberg T, Muzyczka N, Hauswirth WW, Flotte TR, Byrne BJ, Snyder RO (2002) Production and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors. Methods 28:158–167CrossRefPubMed

18.

Harrison JK, Luo D, Streit WJ (2003) In situ hybridization analysis of chemokines and chemokine receptors in the central nervous system. Methods 29:312–318CrossRefPubMed

19.

Qiu J, Ai L, Ramachandran C, Yao B, Gopalakrishnan S, Fields CR, Delmas AL, Dyer LM, Melnick SJ, Yachnis AT, Schwartz PH, Fine HA, Brown KD, Robertson KD (2008) Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas. Lab Invest 88:910–925CrossRefPubMed

20.

Liu C, Luo D, Streit WJ, Harrison JK (2008) CX3CL1 and CX3CR1 in the GL261 murine model of glioma: CX3CR1 deficiency does not impact tumor growth or infiltration of microglia and lymphocytes. J Neuroimmunol 198:98–105CrossRefPubMed

21.

Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW, Kleihues P (2007) The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol 114:97–109CrossRefPubMed

22.

Pescarmona GP, Scalerandi M, Delsanto PP, Condat CA (1999) Non-linear model of cancer growth and metastasis: a limiting nutrient as a major determinant of tumor shape and diffusion. Med Hypotheses 53:497–503CrossRefPubMed

23.

Seiki M (2003) Membrane-type 1 matrix metalloproteinase: a key enzyme for tumor invasion. Cancer Lett 194:1–11CrossRefPubMed

Titel: Hematopoietic- and Neurologic-Expressed Sequence 1 Expression in the Murine GL261 and High-Grade Human Gliomas
verfasst von: Katharine M. Laughlin
Defang Luo
Che Liu
Gerry Shaw
Kenneth H. Warrington Jr.
Jingxin Qiu
Anthony T. Yachnis
Jeffrey K. Harrison
Publikationsdatum: 01.09.2009
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 3/2009
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-008-9147-4

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