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Journal of Cardiovascular Translational Research
Erschienen in: Journal of Cardiovascular Translational Research 4/2017

01.05.2017 | Original Article

Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering

verfasst von: M. Granados, L. Morticelli, S. Andriopoulou, P. Kalozoumis, M. Pflaum, P. Iablonskii, B. Glasmacher, M. Harder, J. Hegermann, C. Wrede, I. Tudorache, S. Cebotari, A. Hilfiker, A. Haverich, Sotirios Korossis

Erschienen in: Journal of Cardiovascular Translational Research | Ausgabe 4/2017

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Abstract

Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.
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Metadaten
Titel
Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering
verfasst von
M. Granados
L. Morticelli
S. Andriopoulou
P. Kalozoumis
M. Pflaum
P. Iablonskii
B. Glasmacher
M. Harder
J. Hegermann
C. Wrede
I. Tudorache
S. Cebotari
A. Hilfiker
A. Haverich
Sotirios Korossis
Publikationsdatum
01.05.2017
Verlag
Springer US
Erschienen in
Journal of Cardiovascular Translational Research / Ausgabe 4/2017
Print ISSN: 1937-5387
Elektronische ISSN: 1937-5395
DOI
https://doi.org/10.1007/s12265-017-9747-z

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