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Emodin-6-O-β-d-glucoside down-regulates endothelial protein C receptor shedding

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Abstract

Endothelial protein C receptor (EPCR) plays an important role in the protein C anticoagulation pathway and in the cytoprotective pathway. Previously, EPCR can be shed from the cell surface, which is mediated by tumor necrosis factor-α converting enzyme (TACE). Soluble EPCR levels are increased in patients with systemic inflammatory diseases. Recently, we reported that a new active compound, emodin-6-O-β-d-glucoside (EG) from Reynoutria japonica, has anti-inflammatory activities. However, little is known of the effects of EG on EPCR shedding. Here, we investigated this issue by monitoring the effects of EG on the phorbol-12-myristate 13-acetate (PMA) or the cecal ligation and puncture (CLP)-mediated EPCR shedding and its underlying mechanisms. Data showed that EG potently inhibited the PMA and CLP-induced EPCR shedding by suppressing TACE expression. Given these results, EG could be used as a candidate therapeutic for the treatment of vascular inflammatory diseases.

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Acknowledgments

This study was supported by the National Research Foundation of Korea (NRF) funded by the Korea government [MEST] (Grant No. 2012-0009400) and by the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Grant No. A111305).

Conflict of interest

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea

    Wonhwa Lee & Jong-Sup Bae

  2. Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Wonhwa Lee

  3. Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Sae-Kwang Ku

Authors
  1. Wonhwa Lee
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  2. Sae-Kwang Ku
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  3. Jong-Sup Bae
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Corresponding author

Correspondence to Jong-Sup Bae.

Additional information

Wonhwa Lee and Sae-Kwang Ku contributed equally to this work.

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Lee, W., Ku, SK. & Bae, JS. Emodin-6-O-β-d-glucoside down-regulates endothelial protein C receptor shedding. Arch. Pharm. Res. 36, 1160–1165 (2013). https://doi.org/10.1007/s12272-013-0114-6

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  • DOI: https://doi.org/10.1007/s12272-013-0114-6

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