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Erschienen in: Indian Journal of Hematology and Blood Transfusion 2/2015

01.06.2015 | Original Article

Detection of Compound Heterozygous of Hb Constant Spring and Hb Q-Thailand by Capillary Electrophoresis and High Performance Liquid Chromatography

verfasst von: Sakorn Pornprasert, Manoo Punyamung

Erschienen in: Indian Journal of Hematology and Blood Transfusion | Ausgabe 2/2015

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Abstract

A capillary electrophoresis (CE) has proven to be superior to a high performance liquid chromatography (HPLC) in the detection of hemoglobin Constant Spring (Hb CS). Thus the aim of this study was to analyze the efficacy of CE and HPLC for the detection of Hb CS in samples with compound heterozygous of Hb CS and Hb Q-Thailand. Hemoglobin analysis was performed in blood samples of 2 patients with compound heterozygous of Hb CS and Hb Q-Thailand by using HPLC and CE. The HPLC chromatogram and CE electrophoregram of the two techniques were compared. Hb CS was not found on HPLC chromatogram while Hb QA2 2 QT δ2), a derivative of Hb Q-Thailand, was presented at the retention time of 4.70–4.80 min and it was close to the retention time of Hb CS. On CE electrophoregram, Hb CS was presented at zone 2 (Z2) and it was distinctly separated from Hb QA2 which was presented at Z1. Therefore, CE was more efficient to the HPLC for diagnosis of compound heterozygous of Hb CS and Hb Q-Thailand.
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Metadaten
Titel
Detection of Compound Heterozygous of Hb Constant Spring and Hb Q-Thailand by Capillary Electrophoresis and High Performance Liquid Chromatography
verfasst von
Sakorn Pornprasert
Manoo Punyamung
Publikationsdatum
01.06.2015
Verlag
Springer India
Erschienen in
Indian Journal of Hematology and Blood Transfusion / Ausgabe 2/2015
Print ISSN: 0971-4502
Elektronische ISSN: 0974-0449
DOI
https://doi.org/10.1007/s12288-014-0411-3

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