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Effect of omega-3 fatty acids on haemostatic functions in urocortin-treated obese rats

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Abstract

Urocortin 1 (UCN1) decreases food intake. We investigated the effects of UCN1 and omega-3 fatty acids (FA) on metabolic and coagulation parameters in high fat diet (HFD)-fed rats. Fifty male Sprague Dawley rats were divided into five groups; control, HFD, HFD with omega-3 FA, HFD with UCN1, and HFD with UCN1 and omega-3 FA. Food intake, body weight (BW), body mass index (BMI), Lee index, glucose, insulin, HOMA-IR, triglycerides, cholesterol, low (LDL) and high (HDL) density lipoproteins, fibrinogen, plasminogen activator inhibitor 1 (PAI-1), fibrin degradation product (FDP), clotting time, bleeding time, prothrombin time (PT), activated partial thromboplastin time (aPTT), and platelet aggregation were measured. Food intake, BW, BMI, Lee index, glucose, insulin, HOMA-IR, triglycerides, cholesterol, LDL, fibrinogen, platelet aggregation, PAI-1, and FDP increased while bleeding and clotting times, PT, and aPTT decreased in HFD rats. UCN1 decreased food intake, BW, BMI, Lee index, bleeding and clotting times, PT, and aPTT and increased fibrinogen, PAI-1, FDP, and platelet aggregation in HFD rats. Omega-3 FA decreased food intake, BW, BMI, Lee index, platelet aggregation, glucose, insulin, HOMA-IR, triglycerides, and increased HDL and bleeding time in HFD rats. We concluded that UCN1 worsens the hypercoagulable state in HFD rats while omega-3 FA improve the insulin resistance and decrease the platelet aggregation in those rats.

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Recommendation

Combination therapy of both UCN1 and omega-3 FA may be used as a possible therapy for obesity as both decrease food intake and omega-3 FA improve the hypercoagulable state which is worsened by UCN1. However, further investigations are needed to evaluate other parameters that may be modified by treating with UCN1 and omega-3 FA.

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  1. Department of Medical Physiology, Faculty of Medicine, Mansoura University, PO box 35516, Mansoura, Egypt

    Ahmed A. El-Gendy & Amr M. Abbas

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  1. Ahmed A. El-Gendy
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Correspondence to Amr M. Abbas.

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El-Gendy, A.A., Abbas, A.M. Effect of omega-3 fatty acids on haemostatic functions in urocortin-treated obese rats. J Physiol Biochem 70, 809–820 (2014). https://doi.org/10.1007/s13105-014-0350-3

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