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Expression of microRNAs associated with oxidative stress in the hippocampus of piglets

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Abstract

Oxidative stress is associated with human diseases and the developmental retardation of animals. The hippocampus is particularly vulnerable to oxidative stress. MicroRNAs (miRNAs), expressed largely in the mammalian brain, are emerging as robust players and have been implicated in many cellular processes. The present study investigated the sub-tissue specificity of miRNA expression in the dorsal hippocampus (DH) and ventral hippocampus (VH) and evaluated the effects of oxidative stress induced by iron dextran (FeDex) treatment on miRNA expression in the DH and VH of pigs using RNA-sequencing technology and bioinformatics, respectively. The results demonstrated that the injection of FeDex significantly increased the levels of several markers of oxidative stress in serum of Rongchang piglets, which indicated that oxidative stress was successfully induced. Sub-tissue specificity was displayed with 54 differentially expressed miRNAs between the VH and DH. The induced oxidative stress emphasized 59 and 46 differentially expressed miRNAs in the DH and VH, respectively. GO and KEGG pathway analyses revealed that the predicted targets of these differentially expressed miRNAs were involved in the pathways that regulate the expression of genes associated with nervous system development, immune response and oxidative stress, which not only revealed the ability of miRNAs to influence complex gene networks in the DH and VH but also further corroborated the successful induction of oxidative stress. Collectively, the results of this study provide a valuable basis for future studies aimed at contributions of miRNAs induced by oxidative stress in growth retardation and neurodegenerative diseases of animals and human.

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Funding

This study was financially supported by the National Natural Science Foundation of China (Grant No. 31201807), the Natural Science Foundation of Chongqing (Grant No. cstc2016jcyjA0282), the Science and Technology Innovation Foundation of Social Livelihood of the People of Chongqing (Grant No. cstc2016shmszx80063), the Fundamental Research Funds for the Central Universities (No. XDJK2016C055) and the Youth fund of Rongchang Campus of Southwest University (No. 20700416).

Authors' contributions

LG conceived of the study and developed the study design. BY and HM carried out the analysis and drafted the manuscript. LG and FZ participated in the study design and drafting of the manuscript. LG contributed to the critical revision of the manuscript. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. The Department of Veterinary Medicine, Rongchang Campus, Southwest University, Rongchang, Chongqing, 402460, China

    Binyu Yang, Hongyuan Mei & Ling Gan

  2. The Department of Animal Husbandry, Rongchang Campus, Southwest University, Rongchang, Chongqing, 402460, China

    Fuyuan Zuo

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  1. Binyu Yang
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  2. Hongyuan Mei
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  4. Ling Gan
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Correspondence to Ling Gan.

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Binyu Yang, Hongyuan Mei, Fuyuan Zuo and Ling Gan have no competing interests exist in the study.

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The experimental procedures followed the actual law of animal protection that was approved by the Animal Care Advisory Committee of Southwest University, China.

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Yang, B., Mei, H., Zuo, F. et al. Expression of microRNAs associated with oxidative stress in the hippocampus of piglets. Genes Genom 39, 701–712 (2017). https://doi.org/10.1007/s13258-017-0537-4

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