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Erschienen in: Tumor Biology 5/2012

01.10.2012 | Research Article

MicroRNA-181a promotes gastric cancer by negatively regulating tumor suppressor KLF6

verfasst von: Xiangyang Zhang, Yuqiang Nie, Yanlei Du, Jie Cao, Bo Shen, Yuyuang Li

Erschienen in: Tumor Biology | Ausgabe 5/2012

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Abstract

MicroRNAs have emerged as crucial regulators of tumorigenesis. However, it remains unknown whether miR-181a is involved in the pathogenesis of gastric cancer. In this study, we found that miR-181a is overexpressed in human gastric cancer tissues. Ectopic expression of miR-181a mimic promoted the proliferation, colony formation, migration, and invasion and inhibited the apoptosis of SGC-7901 gastric cancer cells, whereas ectopic expression of miR-181a inhibitor inhibited the malignant phenotypes of SGC-7901 cells. Site-directed mutagenesis and luciferase reporter assay demonstrated that miR-181a repressed KLF6 expression by targeting its 3′-UTR. Western blot analysis further showed that KLF6 protein was significantly decreased or increased when miR-181a mimic or inhibitor was transfected into SGC-7901 cells, respectively. In summary, these data suggest that KLF6 gene is a direct target of miR-181a and miR-181a functions as an oncomir in gastric cancer by repressing the expression of tumor suppressor KLF6.
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Metadaten
Titel
MicroRNA-181a promotes gastric cancer by negatively regulating tumor suppressor KLF6
verfasst von
Xiangyang Zhang
Yuqiang Nie
Yanlei Du
Jie Cao
Bo Shen
Yuyuang Li
Publikationsdatum
01.10.2012
Verlag
Springer Netherlands
Erschienen in
Tumor Biology / Ausgabe 5/2012
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-012-0414-3

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