Skip to main content
SpringerLink
Log in

Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China

  • Short Communication
  • Published:
Indian Journal of Virology Aims and scope Submit manuscript

Abstract

The aim of the present study was to determine the causative agent of infected swines in the Jilin province of China and assess its genetic characteristics. Virus was isolated from tissues suspected of being infected by porcine reproductive and respiratory syndrome virus (PRRSV) and inoculated onto MARC-145 cells. Virus detection was carried out by RT-PCR, immunofluorescence, electron microscopy and sequencing. The results showed that the isolate was the North American genotype PRRSV, termed the JL-04/12 strain, with a 15,320 bp genome. The homology of the amino acid sequences in two nonstructural proteins and GP2 to GP5, between strains JL-04/12 and HUN4, ranged from 97.2 to 99.3 %. However, JL-04/12 GP6 and N protein were identical in HP-PRRSV JXA1 and HUN4. JL-04/12 was characterized by two discontinuous deletions in Nsp2. We speculate that the isolate is a variant of highly pathogenic porcine reproductive and respiratory syndrome derived from strains in 2006–2008. Altogether, these results indicate that highly pathogenic porcine reproductive and respiratory syndrome virus still exists in the Jilin province of China.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Albina E. Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview. Vet Microbiol. 1997;55:309–16.

    Article  PubMed  CAS  Google Scholar 

  2. Allende R, Lewis TL, Lu Z, Rock DL, Kutish GF, Ali A, Doster AR, Osorio FA. North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions. J Gen Virol. 1999;80(Pt 2):307–15.

    PubMed  CAS  Google Scholar 

  3. Guo B-Q, Chen Z-S, Liu W-X, Cui Y-Z. Isolation and identification of porcine reproductory and respiratory syndrome (PRRS) virus from aborted fetuses suspected of PRRS. Chin Poult Infect Dis. 1996;1–5.

  4. Keffaber K. Reproductive failure of unknown etiology. Am Assoc Swine Pract Newslett. 1989;1:1–10.

    Google Scholar 

  5. Neumann EJ, Kliebenstein JB, Johnson CD, Mabry JW, Bush EJ, Seitzinger AH, Green AL, Zimmerman JJ. Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J Am Vet Med Assoc. 2005;227:385–92.

    Article  PubMed  Google Scholar 

  6. Ropp SL, Wees CE, Fang Y, Nelson EA, Rossow KD, Bien M, Arndt B, Preszler S, Steen P, Christopher-Hennings J, Collins JE, Benfield DA, Faaberg KS. Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States. J Virol. 2004;78:3684–703.

    Article  PubMed  CAS  Google Scholar 

  7. Snijder EJ, Meulenberg JJ. The molecular biology of arteriviruses. J Gen Virol. 1998;79(Pt 5):961–79.

    PubMed  CAS  Google Scholar 

  8. Tian K, Yu X, Zhao T, Feng Y, Cao Z, Wang C, Hu Y, Chen X, Hu D, Tian X, Liu D, Zhang S, Deng X, Ding Y, Yang L, Zhang Y, Xiao H, Qiao M, Wang B, Hou L, Wang X, Yang X, Kang L, Sun M, Jin P, Wang S, Kitamura Y, Yan J, Gao GF. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS ONE. 2007;2(6):e526.

    Article  PubMed  Google Scholar 

  9. Wensvoort G, Terpstra C, Pol JM, ter Laak EA, Bloemraad M, de Kluyver EP, Kragten C, van Buiten L, den Besten A, Wagenaar F, et al. Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Vet Q. 1991;13:121–30.

    Article  PubMed  CAS  Google Scholar 

  10. Yoshii M, Kaku Y, Murakami Y, Shimizu M, Kato K, Ikeda H. Genetic variation and geographic distribution of porcine reproductive and respiratory syndrome virus in Japan. Arch Virol. 2005;150:2313–24.

    Article  PubMed  CAS  Google Scholar 

  11. Zhou Z, Ni J, Cao Z, Han X, Xia Y, Zi Z, Ning K, Liu Q, Cai L, Qiu P, Deng X, Hu D, Zhang Q, Fan Y, Wu J, Wang L, Zhang M, Yu X, Zhai X, Tian K. The epidemic status and genetic diversity of 14 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) isolates from China in 2009. Vet Microbiol. 2011;150:257–69.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the grant of National “Twelfth Five-Year” High Technology Research and Development Program (2011AA10A213), innovation team for special economic animal diseases prevention and control research in Jilin province (No. 20121823), and standard of epidemic diseases in wild animals and tracking study (OIE 2012).

Author information

Authors and Affiliations

  1. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences CAAS, No. 4899 Juye Street, Jilin, 130112, People’s Republic of China

    Feng-Xue Wang, Li Guo, Yan-Ling Yang, Ni Song, Li-Zhi Chen, Shi-Peng Cheng & Yong-Jun Wen

Authors
  1. Feng-Xue Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yan-Ling Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ni Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li-Zhi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shi-Peng Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yong-Jun Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Feng-Xue Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, FX., Guo, L., Yang, YL. et al. Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China. Indian J. Virol. 24, 90–92 (2013). https://doi.org/10.1007/s13337-012-0124-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13337-012-0124-x

Keywords

Search

Navigation