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Erschienen in: Infection 2/2018

31.10.2017 | Original Paper

Assessment of the multiplex PCR-based assay Unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates

verfasst von: Cihan Papan, Melanie Meyer-Buehn, Gudrun Laniado, Thomas Nicolai, Matthias Griese, Johannes Huebner

Erschienen in: Infection | Ausgabe 2/2018

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Abstract

Background

Pneumonia is a major healthcare problem. Rapid pathogen identification is critical, but often delayed due to the duration of culturing. Early, broad antibacterial therapy might lead to false-negative culture findings and eventually to the development of antibiotic resistances. We aimed to assess the accuracy of the new application Unyvero P50 based on multiplex PCR to detect bacterial pathogens in respiratory specimens from children and neonates.

Methods

In this prospective study, bronchoalveolar lavage fluids, tracheal aspirates, or pleural fluids from neonates and children were analyzed by both traditional culture methods and Unyvero multiplex PCR.

Results

We analyzed specimens from 79 patients with a median age of 1.8 (range 0.01–20.1). Overall, Unyvero yielded a sensitivity of 73.1% and a specificity of 97.9% compared to culture methods. Best results were observed for non-fermenting bacteria, for which sensitivity of Unyvero was 90% and specificity 97.3%, while rates were lower for Gram-positive bacteria (46.2 and 93.9%, respectively). For resistance genes, we observed a concordance with antibiogram of 75% for those specimens in which there was a cultural correlate.

Conclusions

Unyvero is a fast and easy-to-use tool that might provide additional information for clinical decision making, especially in neonates and in the setting of nosocomial pneumonia. Sensitivity of the PCR for Gram-positive bacteria and important resistance genes must be improved before this application can be widely recommended.
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Metadaten
Titel
Assessment of the multiplex PCR-based assay Unyvero pneumonia application for detection of bacterial pathogens and antibiotic resistance genes in children and neonates
verfasst von
Cihan Papan
Melanie Meyer-Buehn
Gudrun Laniado
Thomas Nicolai
Matthias Griese
Johannes Huebner
Publikationsdatum
31.10.2017
Verlag
Springer Berlin Heidelberg
Erschienen in
Infection / Ausgabe 2/2018
Print ISSN: 0300-8126
Elektronische ISSN: 1439-0973
DOI
https://doi.org/10.1007/s15010-017-1088-y

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