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Journal of Endocrinological Investigation

Erschienen in:

01.02.2017 | Original Article

High doses of hydroxytyrosol induce apoptosis in papillary and follicular thyroid cancer cells

verfasst von: G. Toteda, S. Lupinacci, D. Vizza, R. Bonofiglio, E. Perri, M. Bonofiglio, D. Lofaro, A. La Russa, F. Leone, P. Gigliotti, R. A. Cifarelli, A. Perri

Erschienen in: Journal of Endocrinological Investigation | Ausgabe 2/2017

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Abstract

Purpose

Recent evidences indicates that hydroxytyrosol, one of the main olive oil phenols, possess antitumor effects because of its pro-oxidant properties and the capacity to inhibit proliferation and to promote apoptosis in several tumor cell lines, although most of the results were obtained for breast and digestive systems cancers.

Methods

In this study, we evaluated the activities of hydroxytyrosol against papillary (TPC-1, FB-2) and follicular (WRO) thyroid cancer cell lines.

Results

Cellular viability revealed that high doses of hydroxytyrosol reduced cancer cells viability concomitantly with a reduction of cyclin D1 expression and an up-regulation of cell cycle key modulator p21 levels. In the same experimental conditions, Annexin V–PI staining and DNA laddering revealed that hydroxytyrosol exerts proapoptotic effects on papillary and follicular cancer cells. Furthermore, by Western blot analysis, we observed that hydroxytyrosol treatment reduced thyroid cancer cells viability by promoting apoptotic cell death via intrinsic pathway.

Conclusions

Collectively, our results demonstrated for the first time that in thyroid cancer cells hydroxytyrosol promoted apoptosis at higher doses with respect to other cancer cells lines. Therefore, further studies will reveal the mechanisms by which thyroid cancer cells are more resistant to the proapoptotic effect exerted by hydroxytyrosol as well as the potential application as novel target therapeutic in thyroid cancer.

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Titel: High doses of hydroxytyrosol induce apoptosis in papillary and follicular thyroid cancer cells
verfasst von: G. Toteda
S. Lupinacci
D. Vizza
R. Bonofiglio
E. Perri
M. Bonofiglio
D. Lofaro
A. La Russa
F. Leone
P. Gigliotti
R. A. Cifarelli
A. Perri
Publikationsdatum: 01.02.2017
Verlag: Springer International Publishing
Erschienen in: Journal of Endocrinological Investigation / Ausgabe 2/2017
Elektronische ISSN: 1720-8386
DOI: https://doi.org/10.1007/s40618-016-0537-2

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High doses of hydroxytyrosol induce apoptosis in papillary and follicular thyroid cancer cells

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

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Results

Conclusions

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