Skip to main content
SpringerLink
Log in

Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

  • Original article
  • Published:
Allergo Journal International Aims and scope Submit manuscript

Abstract

Background

ImmunoCAP ISAC 112, is a commercially available molecular allergy IgE multiplex test. Data on the comparison of this rather novel test with extract-based as well as molecular ImmunoCAP singleplex IgE tests is missing.

Objective

To perform a comparison between the ISAC multiplex IgE assay and the ImmunoCAP singleplex test results.

Methods

Serum samples of 101 adults with grass pollen allergy were analysed for sIgE to 112 allergenic molecules represented on the ISAC test as well as to common atopy-related extract-based allergy tests with the ImmunoCAP System (house dust mite [d1], cat [e1], dog [e5], cow’s milk [f2], hen’s egg [f1], hazelnut [f17], celery [f85], Alternaria alternate [m6], as well as pollen from birch [t3], hazel [t4], mugwort [w6], and ragweed [w1]). Subsequently statistical analysis was performed with the Spearman rank correlation test and the Clopper-Pearson method in order to compare the ISAC multiplex results with the sIgE singleplex results.

Results

The positive percent agreements (PPA) and negative percent agreement (NPA) of corresponding allergens between the ISAC sIgE test and the extract-based singleplex ImmunoCAP results at cutoff 0.1 kUA/l varied between 60–100 % for PPA and 78–97 % for NPA.

Conclusion

When taking into account corresponding allergens molecular testing with the ISAC multiplex test correlates well with ImmunoCAP singleplex results.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1. a-h

Similar content being viewed by others

Abbreviations

CCD:

Cross-reactive carbohydrate determinant

CI:

Confidence interval

CV:

Coefficient of variation

IgE:

Immunglobulin E

ISAC:

Immuno solid-phase allergen chip

ISU:

ISAC standardized unit

ISU-E:

ImmunoCAP specific IgE

kUA:

Kilounits (corresponding allergens)

LoD:

Limit of detection

MA:

Molecular allergy diagnostics

NPA:

Negative percent agreement

PPA:

Positive percent agreement

SD:

Standard deviation

sIgE:

Specific Immunglobulin E

SIT:

Specific Immunotherapy

References

  1. Canonica GW, Ansotegui IJ, Pawankar R, Schmid-Grendelmeier P, van Hage M, Baena-Cagnani CE, Melioli G, Nunes C, Passalacqua G, Rosenwasser L, Sampson H, Sastre J, Bousquet J, Zuberbier T. A WAO - ARIA - GA(2)LEN consensus document on molecular-based allergy diagnostics. World Allergy Organ J 2013;6:17

    Article  PubMed Central  PubMed  Google Scholar 

  2. Szecsi PB, Stender S. Comparison of immunoglobulin E measurements on IMMULITE and ImmunoCAP in samples consisting of allergen-specific mouse-human chimeric monoclonal antibodies towards allergen extracts and four recombinant allergens. Int Arch Allergy Immunol 2013;162:131–4

    Article  CAS  PubMed  Google Scholar 

  3. Wood RA, Segall N, Ahlstedt S, Williams PB. Accuracy of IgE antibody laboratory results. Ann Allergy Asthma Immunol 2007 Jul;99:34–41

    Article  PubMed  Google Scholar 

  4. Cabrera-Freitag P, Goikoetxea MJ, Beorlegui C, Gamboa P, Gastaminza G, Fernandez-Benitez M, Ferrer M, Blanca M, Sanz ML. Can component-based microarray replace fluorescent enzimoimmunoassay in the diagnosis of grass and cypress pollen allergy? Clin Exp Allergy 2011 Oct;41:1440–6

    Article  CAS  PubMed  Google Scholar 

  5. Gadisseur R, Chapelle JP, Cavalier E. A new tool in the field of in-vitro diagnosis of allergy: preliminary results in the comparison of ImmunoCAP(c) 250 with the ImmunoCAP(c) ISAC. Clin Chem Lab Med 2011 Feb;49:277–80

    Article  CAS  PubMed  Google Scholar 

  6. Lizaso MT, Garcia BE, Tabar AI, Lasa E, Echechipia S, Alvarez MJ, Anda M, Gomez B. Comparison of conventional and component-resolved diagnostics by two different methods (Advia-Centaur/Microarray-ISAC) in pollen allergy. Ann Allergy Asthma Immunol 2011 Jul;107:35–41

    Article  PubMed  Google Scholar 

  7. Melioli G, Bonifazi F, Bonini S, Maggi E, Mussap M, Passalacqua G, Rossi ER, Vacca A, Canonica GW. The ImmunoCAP ISAC molecular allergology approach in adult multi-sensitized Italian patients with respiratory symptoms. Clin Biochem 2011 Aug;44:1005–11

    Article  PubMed  Google Scholar 

  8. Ott H, Baron JM, Heise R, Ocklenburg C, Stanzel S, Merk HF, Niggemann B, Beyer K. Clinical usefulness of microarray-based IgE detection in children with suspected food allergy. Allergy 2008 Nov;63:1521–8

    Article  CAS  PubMed  Google Scholar 

  9. Sastre J, Landivar ME, Ruiz-Garcia M, Andregnette-Rosigno MV, Mahillo I. How molecular diagnosis can change allergen-specific immunotherapy prescription in a complex pollen area. Allergy 2012 May;67:709–11

    Article  CAS  PubMed  Google Scholar 

  10. Twaroch TE, Focke M, Fleischmann K, Balic N, Lupinek C, Blatt K, Ferrara R, Mari A, Ebner C, Valent P, Spitzauer S, Swoboda I, Valenta R. Carrier-bound Alt a 1 peptides without allergenic activity for vaccination against Alternaria alternata allergy. Clin Exp Allergy 2012 Jun;42:966–75

    CAS  PubMed  Google Scholar 

  11. Wang J, Godbold JH, Sampson HA. Correlation of serum allergy (IgE) tests performed by different assay systems. J Allergy Clin Immunol 2008 May;121:1219–24

    Article  CAS  PubMed  Google Scholar 

  12. Ebo DG, Hagendorens MM, De Knop KJ, Verweij MM, Bridts CH, De Clerck LS, Stevens WJ. Component-resolved diagnosis from latex allergy by microarray. Clin Exp Allergy 2010 Feb;40:348–58

    Article  CAS  PubMed  Google Scholar 

  13. Martinez-Aranguren R, Lizaso MT, Goikoetxea MJ, Garcia BE, Cabrera-Freitag P, Trellez O, Sanz ML. Is the determination of specific IgE against components using ISAC 112 a reproducible technique? PLoS One 2014;9:e88394

    Article  PubMed Central  PubMed  Google Scholar 

  14. Darsow U, Brockow K, Pfab F, Jakob T, Petersson CJ, Borres MP, Ring J, Behrendt H, Huss-Marp J. Heterogeneity of molecular sensitization profiles in grass pollen allergy implications for immunotherapy? Clin Exp Allergy 2014 Mar 6

    Google Scholar 

  15. Huss-Marp J, Darsow U, Brockow K, Pfab F, Weichenmeier I, Schober W, Petersson CJ, Borres MP, Ring J, Behrendt H. Can immunoglobulin E-measurement replace challenge tests in allergic rhinoconjunctivits to grass pollen? Clin Exp Allergy 2011 Aug;41:1116–24

    Article  CAS  PubMed  Google Scholar 

  16. Ferrer M, Sanz ML, Sastre J, Bartra J, del Cuvillo A, Montoro J, Jauregui I, Davila I, Mullol J, Valero A. Molecular diagnosis in allergology: application of the microarray technique. J Investig Allergol Clin Immunol 2009;19 Suppl 1:19–24

    CAS  PubMed  Google Scholar 

  17. Renault NK, Mirotti L, Alcocer MJ. Biotechnologies in new high-throughput food allergy tests: why we need them. Biotechnol Lett 2007 Mar;29:333–9

    Article  CAS  PubMed  Google Scholar 

  18. Sanz ML, Blazquez AB, Garcia BE. Microarray of allergenic component-based diagnosis in food allergy. Curr Opin Allergy Clin Immunol 2011 Jun;11:204–9

    Article  CAS  PubMed  Google Scholar 

  19. Ahlgrim C, Gutermuth J, Ænell A, Borres MP, Schäffner I, Darsow U et. al Comparison of molecular multiplex and singleplex analysis of IgE to grass pollen allergens in untreated German grass pollen allergic patients. J Invest All Clin Immunol 2015; Vol 25 (in press)

Download references

Author information

Authors and Affiliations

  1. Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany

    Johannes Huss-Marp (Medical Unit Leader Dermatology) & Thilo Jakob

  2. Thermo Fisher Scientific, Phadia GmbH, Freiburg, Germany

    Johannes Huss-Marp (Medical Unit Leader Dermatology) & Ina Schäffner

  3. Department of Dermatology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium

    Jan Gutermuth

  4. Department of Dermatology and Allergy, Technische Universität München, Munich, Germany

    Ulf Darsow, Florian Pfab, Knut Brockow & Johannes Ring

  5. ZAUM-Center for Allergy and Environment, Helmholtz Center Munich, Technische Universität München, Munich, Germany

    Ulf Darsow, Florian Pfab, Knut Brockow & Heidrun Behrendt

  6. University Freiburg Medical Center, Department of Exercise Medicine and Sport, University of Freiburg, Freiburg, Germany

    Christoph Ahlgrim

  7. Department of Pneumology, University of Freiburg, Freiburg, Germany

    Christoph Ahlgrim

  8. Department of Pneumology, Freiburg University Medical Center, Albert-Ludwigs-University, Freiburg, Germany

    Christoph Ahlgrim

  9. AbbVie Deutschland GmbH & Co. KG, Mainzer Straße 81, 65189, Wiesbaden, Germany

    Johannes Huss-Marp (Medical Unit Leader Dermatology)

Authors
  1. Johannes Huss-Marp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jan Gutermuth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ina Schäffner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ulf Darsow
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Florian Pfab
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Knut Brockow
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Johannes Ring
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Heidrun Behrendt
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Thilo Jakob
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Christoph Ahlgrim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Johannes Huss-Marp.

Additional information

Author contributions (as proposed by International Committee of Medical Journal Editors [ICMJE])

J. Huss-Marp: design of the study, data collection, statistical analysis, preparation and revision of the manuscript; J. Gutermuth: data collection and revision of the manuscript; I. Schäffner: preparation and revision of the manuscript; U. Darsow: data collection and revision of the manuscript; F. Pfab: data collection and revision of the manuscript; K. Brockow: data collection and revision of the manuscript; J. Ring: design of the study and revision of the manuscript; H. Behrendt: design of the study and revision of the manuscript; T. Jakob: data collection and revision of the manuscript; C. Ahlgrim: statistical analysis, preparation and revision of the manuscript

Cite this as: Huss-Marp J, Gutermuth J, Schäffner I, Darsow U, Pfab F, Brockow K, Ring J, Behrendt H, Jakob T, Ahlgrim C. Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis. Allergo J Int 2015;24:46–53 DOI: 10.1007/s40629-015-0045-5

Funding

This work was supported by a research grant from Thermo Fisher Scientific, Phadia GmbH, Freiburg

Conflict of interest

Schäffner I and Huss-Marp J were employees of Thermo Fisher Scientific. Jakob T has received honoraria for consulting and for serving on the speakers buro of Thermo Fisher Scientific and has received research funding from Thermo Fisher Sceintific. The other authors state that there are no conflicts of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huss-Marp, J., Gutermuth, J., Schäffner, I. et al. Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis. Allergo J Int 24, 46–53 (2015). https://doi.org/10.1007/s40629-015-0046-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40629-015-0046-4

Keywords

Search

Navigation