The effect of 2,4-dinitrophenol on the turnover of the acid-soluble phosphorus of rat diaphragm☆

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Abstract

The effects of dinitrophenol (DNP) on phosphorylation have been studied in the isolated rat diaphragm. Three concentrations of DNP were used: one which stimulated respiration, a higher one which gave initial stimulation followed by depression, and a still higher one which gave principally profound depression. With all concentrations of DNP phosphocreatine was completely hydrolyzed and the concentration of adenosine triphosphate (ATP) was reduced markedly. The concentration of hexose monophosphate (HMP) was reduced when respiration was depressed.

The turnover rate of ATP was not affected by a concentration of DNP which stimulated respiration, but was decreased by the higher concentrations which depressed respiration.

The turnover of HMP was increased by the stimulating concentration and not reduced below the control level by the higher concentrations of DNP. This suggests that there are pathways for the formation of HMP in the intact cell which are resistant to DNP and therefore different from those found in particulate fractions.

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Research carried out at Brookhaven National Laboratory under the auspices of the U. S. Atomic Energy Commission.

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With the technical assistance of Marie Pancirov and Jean MacMullen.

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