A new form of amyloid protein associated with chronic hemodialysis was identified as β2-microglobulin

https://doi.org/10.1016/0006-291X(85)91948-5Get rights and content

Abstract

Amyloid fibrils were isolated from amyloid-laden tissue obtained from a chronic hemodialysis patient with carpal tunnel syndrome. After solubilization in guanidine HCl, a significant amount of the protein was located in a homogeneous low molecular weight fraction. The protein was found to be identical to β2-microglobulin, with regard to its molecular weight of 11,000, amino acid composition and 16 amino-terminal amino acids: Ile-Gln-Arg-Thr-Pro-Lys-Ile-Gln-Val-Tyr-Ser-Arg-His-Pro-Ala-Glu-. These results demonstrate that the amyloid associated with chronic hemodialysis contains as major component a new form of amyloid fibril protein that is homologous to β2-microglobulin.

References (24)

  • G.G. Glenner et al.

    Prep. Biochem

    (1972)
  • F. Hashimoto et al.

    Anal. Biochem

    (1983)
  • L.K. Laemmli

    Nature

    (1970)
  • C. Vincent et al.

    Nephron

    (1978)
  • A. Schwarz et al.

    Clin. Nephrol

    (1984)
  • F. Spertini et al.

    Clin. Nephrol

    (1984)
  • J.A. Delmez et al.

    Nephron

    (1982)
  • S.K. Halter et al.

    Arch. Phys. Med. Rehabil

    (1981)
  • V.K. Jain et al.

    J. Am. Med. Assoc

    (1979)
  • H.G. Kachel et al.

    Contr. Nephrol

    (1983)
  • H. Assenat et al.

    Nouv. Presse Med

    (1980)
  • R. Durroux et al.

    Nouv. Presse Med

    (1981)
  • Cited by (0)

    View full text