Mammalian testes in organ culture☆
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Experimental models of testicular development and function using human tissue and cells
2018, Molecular and Cellular EndocrinologyCitation Excerpt :The establishment of in vitro culture systems to model human testicular function by investigating testis development, spermatogenesis and steroidogenesis is not a new idea. The first experiments were reported in 1937 (Martinovitch, 1937) and in the 1960s and 1970s Steinberger and colleagues pioneered work on tissue culture of testicular tissue (Steinberger, 1975; Steinberger and Steinberger, 1965, 1970; Steinberger et al., 1964a; Steinberger et al., 1964b). This approach has since provided important information about the specific roles of different cell types within the human testis, their regulation and cell-cell interactions within the niche.
In vitro spermatogenesis
2018, Encyclopedia of ReproductionResveratrol supports and alpha-naphthoflavone disrupts growth of human ovarian follicles in an in vitro tissue culture model
2018, Toxicology and Applied PharmacologyCitation Excerpt :All reagents were purchased from Life Technologies (Grand Island, NY, USA), with the exception of human serum albumin solution (Vitrolife, Göteborg, Sweden) and FSH (Serono Nordic, Solna, Sweden). Culture conditions mimicked the air-liquid interphase system reported for mouse testes (Steinberger et al., 1964; Sato et al., 2011). A 0.5% gel was prepared by dissolving agarose in ddH2O.
Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis
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Supported by NIH Grant No. 5445.