Connective tissue changes in early ischemia of porcine myocardium: An ultrastructural study

https://doi.org/10.1016/0022-2828(83)90281-XGet rights and content

Abstract

Scanning electron microscopy and transmission electron microscopy were used together with tannic acid and ruthenium-red staining to examine connective tissue damage caused by acute myocardial ischemia for 20, 40 and 120 min in pig hearts. The microsphere blood flow technique revealed that blood flow was approximately 0.02 ml/min/g in inner, middle and outer thirds of the ischemic zone. After 20 min of occlusion of the left anterior descending coronary artery, the collagen network and microfilaments became irregularly arranged. After 40 min of occlusion, ruthenium-red positive glyco-protein material around the collagen fibrils and elastin began to disappear. After 2 h occlusion, the collagen fibrils and microfilaments had separated from the basement membrane. Collagen fibrils, elastic fibers, and microfilaments were broken down and were found in decreased quantities. These results have revealed that the connective tissue remains intact during the first 20 min of coronary occlusion despite zero blood flow and mild cellular changes but does undergo prominent alterations after 40 min of occlusion.

References (38)

  • G.D. Buckberg et al.

    Some sources of error in measuring regional blood flow with radioactive microspheres

    J Appl Physiol

    (1971)
  • M. Ashraf et al.

    Surface ultrastructural features of isolated perfused rat hearts during calcium paradox

    Scan Electron Microsc

    (1981)
  • J.B. Caulfield et al.

    The collagen network of the heart

    Lab Invest

    (1979)
  • H. Fujiwara et al.

    Transmural cellular damage and blood flow distribution in the early ischemia of left ventricular wall in pig

    Circ Res

    (1982)
  • H. Fujiwara et al.

    Prevention of myocardial cellular damage during ischemia by diltiazem (DTZ) pretreatment

  • J.M. Goslin

    The physical properties of elastic tissue

    Int Rev Connect Tissue Res

    (1979)
  • T.K. Greenlee et al.

    The fine structure of elastic fiber

    J Cell Biol

    (1966)
  • D.M. Griggs et al.

    Transmural differences in ventricular tissue substrate levels due to coronary constriction

    Am J Physiol

    (1972)
  • P.B. Herdson et al.

    A comparative study of the fine structure of normal and ischemic dog myocardium with special reference to early changes following temporary occlusion of a coronary artery

    Am J Pathol

    (1965)
  • Cited by (0)

    Present address: Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-Ku, Tokyo, 113 Japan.

    View full text