Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans

doi:10.1016/0092-8674(93)90530-4

Cell

Volume 75, Issue 5, 3 December 1993, Pages 855-862

https://doi.org/10.1016/0092-8674(93)90530-4 Get rights and content

Abstract

During C. elegans development, the temporal pattern of many cell lineages is specified by graded activity of the heterochronic gene Lin-14. Here we demonstrate that a temporal gradient in Lin-14 protein is generated posttranscriptionally by multiple elements in the lin-14 3′UTR that are regulated by the heterochronic gene Lin-4. The lin-14 3′UTR is both necessary and sufficient to confer lin-4-mediated posttranscriptional temporal regulation. The function of the lin-14 3′UTR is conserved between C. elegans and C. briggsae. Among the conserved sequences are seven elements that are each complementary to the lin-4 RNAs. A reporter gene bearing three of these elements shows partial temporal gradient activity. These data suggest a molecular mechanism for Lin-14p temporal gradient formation: the lin-4 RNAs base pair to sites in the lin-14 3′UTR to form multiple RNA duplexes that down-regulate lin-14 translation.

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^★: The first two authors contributed equally to this work.

^†: Present address: Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720

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References (27)

A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans

Cell

Mutations that lead to reiterations in the cell lineages of C. elegans

Cell

Sequence analysis of the C. elegans myosin heavy chain family

J. Mol. Biol.

Activation of double-stranded RNA-dependent kinase (dsl) by the TAR region of HIV-1 mRNA: a novel translational control mechanism

Cell

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Gene

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Cell

Regulating the fate of mRNA: the control of cellular iron metabolism

Cell

CeMyoD accumulation defines the body wall muscle cell fate during C. elegans embryogenesis

Cell

The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation

Cell

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J. Mol. Biol.

RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos

Cell

In the beginning is the end: regulation of poly(A) addition and removal during early development

Trends Biochem. Sci.

Heterochronic mutants of the nematode Caenorhabditis elegans

Science

Cited by (3329)

Biomarker potential of competing endogenous RNA networks in Polycystic Ovary Syndrome (PCOS)

Emerging role of tumor suppressing microRNAs as therapeutics in managing non-small cell lung cancer

microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways

Antimonene-based surface plasmon resonance with antibody S9.6 signal amplification for miRNA detection

Strategies for mitigation of pesticides from the environment through alternative approaches: A review of recent developments and future prospects

miRNAs as biomarkers of therapeutic response to HER2-targeted treatment in breast cancer: A systematic review

Cell

ArticlePosttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans

Abstract

Cell

Cell

J. Mol. Biol.

Cell

Gene

Cell

Cell

Cell

Cell

J. Mol. Biol.

Cell

Trends Biochem. Sci.

Heterochronic mutants of the nematode Caenorhabditis elegans

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Article
Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans