Heterogeneity of grass pollen allergens (Dactylis glomerata) recognized by IgE antibodies in human patients sera by a new nitrocellulose immunoprint technique

doi:10.1016/0165-2478(82)90096-7

Immunology Letters

Volume 5, Issue 3, September 1982, Pages 127-131

https://doi.org/10.1016/0165-2478(82)90096-7 Get rights and content

Abstract

A new nitrocellulose immunoprint technique has been developed to detect specific antigens or/and allergens present among a heterogeneous solution such as a water-soluble crude extract of a grass pollen (Dactylis glomerata). The antigens are separated by isoelectric focusing (IEF) in an agarose gel and characterized by their isoelectric point (pI). These antigens are transferred and immobilized on a nitrocellulose sheet. They are recognized by the binding of specific antibodies contained in an unfractionated serum to be studied.

Finally, the binding of these antibodies is visualized by species- or/and class-specific antibodies themselves labeled by an enzyme or by radioactivity.

So one can detect the allergens recognized by the specific serum IgE antibodies and also the other antigens recognized by specific IgG, IgA or IgM antibodies.

References (8)

L. Wide et al.
Lancet
(1967)
E.M. Southern
J. Mol. Biol.
(1975)
N.E. Ericksson et al.
Int. Arch. Allergy Appl. Immunol.
(1977)
J.L. Guesdon et al.
Clin. Exp. Immunol.
(1978)

There are more references available in the full text version of this article.

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One gram of native and defatted Dactylis glomerata pollen (Allergon, Angelholm, Sweden) was incubated for 1h at 22 °C in 9 ml of distilled water in a drum (Rotator, Stuart Barleworld Scientific, Nemours, France) rotating at 22 rpm (Peltre et al., 1982).
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Heterogeneity of grass pollen allergens (Dactylis glomerata) recognized by IgE antibodies in human patients sera by a new nitrocellulose immunoprint technique

Abstract

Lancet

J. Mol. Biol.

Int. Arch. Allergy Appl. Immunol.

Clin. Exp. Immunol.