A beneficial effect of trypsin on the purification of turnip mosaic virus (TuMV) and other potyviruses

doi:10.1016/0166-0934(88)90040-7

Journal of Virological Methods

Volume 20, Issue 1, May 1988, Pages 57-64

https://doi.org/10.1016/0166-0934(88)90040-7 Get rights and content

Abstract

The effects of treatment with trypsin during the purification of turnip mosaic virus (TuMV), on virus yield, infectivity and integrity of virus coat protein were examined. Trypsin increased yield markedly, and at a low concentration, increased infectivity. These effects were probably due to reduced aggregation of virus particles. At higher concentrations of trypsin, there was some degradation of virus coat protein, and infectivity was reduced.

Treatment with trypsin at the optimum concentration can significantly improve purification of TuMV; more limited experiments suggest that it can also be applied to other potyviruses.

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Citation Excerpt :
Methods for TuMV purification have been described by Shepherd and Pound (1960) and Tomlinson (1964). Some modifications were later proposed by Thompson et al. (1988). The virus is transmitted in a nonpersistent manner by over 40 aphid species, particularly by M. persicae, A. craccivora, and M. euphorbiae.
Lettuce is frequently attacked by several viruses causing disease epidemics and considerable yield losses along the Mediterranean basin. Aphids are key pests and the major vectors of plant viruses in lettuce fields. Lettuce mosaic virus (LMV) is probably the most important because it is seed-transmitted in addition to be transmissible by many aphid species that alight on the crop. Tomato spotted wilt virus (TSWV) is another virus that causes severe damage since the introduction of its major vector, the thrips Frankliniella occidentalis. In regions with heavy and humid soils, Lettuce Mirafiori big-vein virus (LMBVV) can also produce major yield losses.
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¹: Current addresses: Institute of Horticultural Research, Worthing Road, Littlehampton, West Sussex, BN17 6LP, U.K.

²: Current addresses: Chaundlers Cottage, The Street, North Warnborough, Basingstoke, Hants RG25 1BG, U.K.

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Journal of Virological Methods

Abstract

References (9)

Biochemical properties of turnip mosaic virus

Virology

Towards a system for the identification and classification of potyviruses

Virology

Purification of the tobacco etch and other viruses of the potato Y group

Phytopathology

Resistance to bean common mosaic virus in Phaseolus vulgaris

Cited by (16)

Virus Diseases in Lettuce in the Mediterranean Basin

Advances in the development of antivirals for rotavirus infection

Infection of Colletotrichum acutatum and Phytophthora infestans by taxonomically different plant viruses

High variability in the N terminus of coat protein among potyviruses and its advantage in producing a specific antibody

Interactions of liver and heart

Occurrence of turnip mosaic virus in Phalaenopsis sp. in China