Detection of porcine reproductive respiratory syndrome virus by the polymerase chain reaction
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Testicular immune tolerance and viral infections
2022, Translational Autoimmunity: Challenges for Autoimmune Diseases: Volume 5Rapid and sensitive detection of type II porcine reproductive and respiratory syndrome virus by reverse transcription loop-mediated isothermal amplification combined with a vertical flow visualization strip
2014, Journal of Virological MethodsCitation Excerpt :In our study, the reaction tubes were incubated at 61 °C for 40 min and then directly analyzed by using the VF visualization strip cassette. Importantly, the RT-LAMP-VF assay exhibits high specificity and sensitivity within 50 min, which is much rapid than RT-PCR used for PRRSV detection (Van Woensel et al., 1994; Chittick et al., 2011; Wernike et al., 2012) that takes at least 2 h. To the best of our knowledge, this is the first study to describe the RT-LAMP assay in combination with a sealed lateral flow strip to avoid any contamination during the detection of type II PRRSV. Moreover, the requirement for only limited equipment is another advantage of this method, making it applicable for rapid veterinary diagnosis and for use in rural areas.
Multiplex PCR for the simultaneous detection of porcine reproductive and respiratory syndrome virus, classical swine fever virus, and porcine circovirus inpigs
2013, Molecular and Cellular ProbesCitation Excerpt :The PCR is an alternative rapid virus detection method and several single PCR-based methods have been reported for a number of porcine pathogens. Individual PCR or reverse transcriptase (RT)-PCR assays have been developed for detection and identification of PRRSV, CSFV, and PCV-2 [14–16]. However, using conventional PCR technology to detect several viruses individually is labour intensive and expensive.
Rapid detection of porcine reproductive and respiratory syndrome virus by reverse transcription loop-mediated isothermal amplification assay
2009, Journal of Virological Methods