Primary structure and localization of a conserved immunogenicPlasmodium falciparum glutamate rich protein (GLURP) expressed in both the preerythrocytic and erythrocytic stages of the vertebrate life cycle

doi:10.1016/0166-6851(91)90135-S

Molecular and Biochemical Parasitology

Volume 49, Issue 1, November 1991, Pages 119-131

https://doi.org/10.1016/0166-6851(91)90135-S Get rights and content

Abstract

A gene coding for a 220-kDa glutamate rich protein (GLURP), an exoantigen ofPlasmodium falciparum, was isolated and its nucleotide sequence was determined. The deduced amino acid sequence contains 2 repeat regions. The sequence of one of these was shown to be conserved among geographically dispersed isolates, and a fusion protein containing that sequence was able to stimulate B- and T-cells. Antibodies against GLURP stained erythrocytic stages of the parasite as well as the hepatic stage as detected by electron microscopy.

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Primary structure and localization of a conserved immunogenicPlasmodium falciparum glutamate rich protein (GLURP) expressed in both the preerythrocytic and erythrocytic stages of the vertebrate life cycle

Abstract

Int. J. Parasitol.

Trans. R. Soc. Trop. Med. Hyg.

Mol. Biochem. Parasitol.

Exp. Parasitol.

Exp. Parasitol.

Trans. R. Soc. Trop. Med. Hyg.

Gene

Anal. Biochem.

Exp. Parasitol.

Mol. Biochem. Parasitol.

Mol. Biochem. Parasitol.

Immunoadsorbent isolation of antigens from the culture medium of in vitro cultivatedPlasmodium falciparum

Acta Pathol. Microbiol. Scand. Sect. C

Antigens and antibodies inPlasmodium falciparum malaria studied by immunoelectrophoretic methods

Acta Pathol. Microbiol. Scand. Sect. C

Cell-mediated immune responses toPlasmodium falciparum antigens in adult Gambians

Clin. Exp. Immunol.

Induction of protective immunity toPlasmodium falciparum inSaimiri sciureus monkeys with partially purified exoantigens

Infect. Immun.

Characterization of a 96-kilodalton thermostable polypeptide antigen ofPlasmodium falciparum related to protective immunity in the squirrel monkey

Infect. Immun.

Inhibition of in vitro growth ofPlasmodium falciparum by purified antimalarial human IgG antibodies

Scand. J. Immunol.

Plasmodium falciparum antigens synthesized by schizonts and stabilized at the merozoite surface by antibodies when schizonts mature in the presence of growth inhibitory immune serum

J. Immunol.

Monoclonal antibody characterization ofPlasmodium falciparum antigens in immune complexes formed when schizonts rupture in the presence of immune serum

J. Immunol.

Specificities of antibodies that inhibit merozoite dispersal from malaria-infected erythrocytes

Mol. Biochem. Parasitol.

APlasmodium falciparum antigen that binds to host erythrocytes and merozoites

Science

Molecular Cloning

Efficient isolation of genes by using antibody probes

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

Gene

Construction of a new family of high efficiency of a new family of bacterial expression vectors: Identification of cDNA clones coding for human liver proteins

EMBO J.