Analysis of sequence diversity in the Plasmodium falciparum merozoite surface protein-1 (MSP-1)

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Abstract

Immunization with the first identified Plasmodium falciparum merozoite surface protein (MSP-1) protected monkeys from an otherwise fatal infection. The question of whether the high degree of diversity in MSP-1 among parasite clones will be an impediment to its development as a vaccine candidate needs to be resolved. We have aligned all published sequences, identifying errors, resequencing a portion of one parasite clone, and identifying probable duplicate sequences of four pairs of parasite clones. The sequences are displayed in a fashion that facilitates the study of variation and its potentially diverse origins. The original dimorphic sequences described by Tanabe et al. [22] have been modified to include only common sequences throughout the entire gene. The extension of the dimorphic region to the 5′ end of block 3 brings into question the involvement of intragenic crossover as the major mechanism generating allelic diversity. Additional diversity developed from point mutations and recombination in certain regions of the gene. The regions of variability and conservation should serve as a data base for planning vaccine trials.

Reference (42)

  • CooperJ.A. et al.

    Mapping of the region predominantly recognized by antibodies to thePlasmodium falciparum merozoite surface antigen MSA 1

    Mol. Biochem. Parasitol

    (1992)
  • ErlichH.A. et al.

    Shared epitopes among HLA class II alleles: Gene conversion, common ancestry and balancing selection

    Immunol. Today

    (1991)
  • HallR. et al.

    Major surface antigen gene of a human malaria parasite cloned and expressed in bacteria

    Nature

    (1984)
  • SiddiquiW.A. et al.

    Merozoite surface coat precursor protein completely protectsAotus monkeys againstPlasmodium falciparum malaria

  • EtlingerH.M. et al.

    Ability of recombinant or native proteins to protect monkeys against heterologous challenge withPlasmodium falciparum

    Infect. Immun.

    (1991)
  • HolderA.A. et al.

    Immunization against blood-stage rodent malaria using purified parasite antigens

    Nature

    (1981)
  • FreemanR.R. et al.

    Characteristics of the protective response of BALB/c mice immunized with a purifiedPlasmodium yoelii schizont antigen

    Clin. Exp. Immunol.

    (1983)
  • MajarianW.R. et al.

    Passive protection against murine malaria with an IgG3 monoclonal antibody

    J. Immunol.

    (1984)
  • BurnsJ.M. et al.

    A protective monoclonal antibody recognizes an epitope in the carboxylterminal cysteine-rich domain in the precursor of the major merozoite surface antigen of the rodent malarial parasite,Plasmodium yoelii

    J. Immunol.

    (1989)
  • BurnsJ.M. et al.

    A protective monoclonal antibody recognizes a variant-specific epitope in the precursor of the major merozoite surface antigen of the rodent malarial parasitePlasmodium yoelii

    J. Immunol.

    (1989)
  • HolderA.A. et al.

    Primary structure of the precursor to the three major surface antigens ofPlasmodium falciparum merozoites

    Nature

    (1985)
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